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Functional black fungus and kudzuvine root food and preparation method thereof

A technology of functional food and black fungus, applied in the field of bioengineering, can solve the problems of not being able to make full use of kudzu root resources, reduce the use effect, and environmental pollution, and achieve the effects of easy operation, simple equipment structure, and low investment

Inactive Publication Date: 2013-10-02
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are two main methods of using kudzu root: one is to extract the active substances of kudzu root to make health products or medicines, but the extraction method not only cannot make full use of kudzu root resources, but also easily leads to environmental pollution; the other is to grind kudzu root directly Adding food or selling it as a health product, this method is worth food or medicine, there are difficulties in human body absorption, etc., which reduces the use effect

Method used

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  • Functional black fungus and kudzuvine root food and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Weigh 20g of sucrose, 50g of soybean cake powder, 50g of corn steep liquor, 60g of bran, 0.6g of potassium dihydrogen phosphate, 0.5g of magnesium sulfate, and 1000ml of water, and mix them to obtain a culture medium. Sterilize at 100°C for 10 minutes, and after cooling, insert 3 pieces of slanted strains of black fungus test tubes of 5×5mm, and cultivate them at a rotation speed of 60r / min and a temperature of 22°C for 5 days to prepare black fungus liquid shake flask strains.

[0023] The liquid submerged medium is prepared by mixing 100g of sucrose, 10g of soybean cake powder, 10g of corn steep liquor, 10g of bran, 0.6g of potassium dihydrogen phosphate, 0.5g of magnesium sulfate, 7g of soybean oil, and 1000ml of water, according to 50% of the volume of the seed tank Insert the liquid submerged culture medium, sterilize at 100°C, cool to room temperature, add black fungus liquid shake flask strains according to 3% of the volume of the seed tank, at a temperature of 22...

Embodiment 2

[0026] Weigh 60g of sucrose, 30g of soybean cake powder, 30g of corn steep liquor, 30g of bran, 0.3g of sodium dihydrogen phosphate, 0.3g of magnesium sulfate, and 1000ml of water, and mix them to prepare a culture medium. Sterilize at 120°C for 30 minutes, and after cooling, insert 4 pieces of slant strains of black fungus test tubes of 5×5mm, and cultivate them at a rotation speed of 120r / min and a temperature of 25°C for 8 days to prepare black fungus liquid shake flask strains.

[0027] The liquid submerged medium is prepared by mixing 60g of sucrose, 25g of soybean cake powder, 31g of corn steep liquor, 30g of bran, 0.25g of sodium dihydrogen phosphate, 0.25g of magnesium sulfate, 4.1g of soybean oil, and 1000ml of water. %Introduce liquid submerged medium, sterilize at 110°C, cool to room temperature, add black fungus liquid shake flask strains according to 8% of the volume of the seed tank, at a temperature of 25°C, a tank pressure of 0.05MPa, and a stirring speed of 180...

Embodiment 3

[0030] Weigh 100g of sucrose, 10g of soybean cake powder, 10g of corn steep liquor, 10g of bran, 0.06g of diammonium hydrogen phosphate, 0.05g of magnesium sulfate, and 1000ml of water, and mix them to prepare a culture medium. Sterilize at 130°C for 60 minutes, and after cooling, insert 3 pieces of slanted strains of black fungus test tubes of 5×5mm, and cultivate them at a rotation speed of 180r / min and a temperature of 33°C for 10 days to prepare black fungus liquid shake flask strains.

[0031]The liquid submerged medium is prepared by mixing 20g of sucrose, 45g of soybean cake powder, 45g of corn steep liquor, 60g of bran, 0.06g of diammonium hydrogen phosphate, 0.05g of magnesium sulfate, 2g of soybean oil, and 1000ml of water, according to 80% of the volume of the seed tank Insert the liquid submerged culture medium, sterilize at 120°C, cool to room temperature, add black fungus liquid shake flask strains according to 10% of the volume of the seed tank, at a temperature ...

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Abstract

The invention discloses a preparation method of a functional black fungus and kudzuvine root food. The method comprises the following steps of: (1) preparing strains in the manner of shaking liquids in a bottle; (2) preparing the strains from a black fungus liquid in a submerged cultivation manner; (3) preparing the functional black fungus and kudzuvine root food. For the prepared functional black fungus and kudzuvine root food, the content of total flavonoids is within 3 to 20mg / g; the content of puerarin is within 0.2 to 1.5mg / g; the content of protein is within 0.9 to 8mg / g; the content of revertose is within 0 to 0.5%; the content of polysaccharide is within 1 to 15mg / g. In addition, the content of the revertose cannot be equal to zero. The food has the functions of resisting oxidation and lowering blood pressure, and has the certain treatment effects for resisting oxidation and lowering blood pressure. The prepared food has a slight fruity flavor and a special aroma of black fungus fermentation. According to the method, the metabolite concentration is high; the fermentation process is not limited strictly, namely, a strict aseptic condition is not required. The equipment is simple in structure, small in investment, low in energy consumption and easy to operate. The method is simple and convenient in aftertreatment and generates little pollution; the wastewater is nearly not discharged.

Description

technical field [0001] The invention relates to a food and a preparation method thereof, in particular to a black fungus kudzu root functional food and a preparation method thereof, belonging to the technical field of bioengineering. Background technique [0002] Pueraria lobata is the dried root of the leguminous plant Pueraria mirifica or kudzu vine. Pueraria lobata is mainly produced in Hunan, Henan, Guangdong, Zhejiang, Sichuan and other provinces; kudzu vines are mostly cultivated, mainly produced in Guangxi, Guangdong and other provinces, and also produced in Sichuan and Yunnan. High-performance liquid chromatography analysis shows that the main active ingredients of puerarin are flavonoids represented by puerarin, followed by a small amount of puerarin (SA1~SA4), puerarin SB1, puerarin (A1~A5) and puerarin C1, etc. Triterpenoids, and trace amounts of choline chloride, acetylcholine dichloride, longsein, D mannitol, L (+) magnesium lactate, β sitosterol, carotene, 6,7...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/29A23L1/214A23L1/28A23L31/00C12N1/14
Inventor 贾君曹煜谢春芹
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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