Functional black fungus and kudzuvine root food and preparation method thereof
A technology of functional food and black fungus, applied in the field of bioengineering, can solve the problems of not being able to make full use of kudzu root resources, reduce the use effect, and environmental pollution, and achieve the effects of easy operation, simple equipment structure, and low investment
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Embodiment 1
[0022] Weigh 20g of sucrose, 50g of soybean cake powder, 50g of corn steep liquor, 60g of bran, 0.6g of potassium dihydrogen phosphate, 0.5g of magnesium sulfate, and 1000ml of water, and mix them to obtain a culture medium. Sterilize at 100°C for 10 minutes, and after cooling, insert 3 pieces of slanted strains of black fungus test tubes of 5×5mm, and cultivate them at a rotation speed of 60r / min and a temperature of 22°C for 5 days to prepare black fungus liquid shake flask strains.
[0023] The liquid submerged medium is prepared by mixing 100g of sucrose, 10g of soybean cake powder, 10g of corn steep liquor, 10g of bran, 0.6g of potassium dihydrogen phosphate, 0.5g of magnesium sulfate, 7g of soybean oil, and 1000ml of water, according to 50% of the volume of the seed tank Insert the liquid submerged culture medium, sterilize at 100°C, cool to room temperature, add black fungus liquid shake flask strains according to 3% of the volume of the seed tank, at a temperature of 22...
Embodiment 2
[0026] Weigh 60g of sucrose, 30g of soybean cake powder, 30g of corn steep liquor, 30g of bran, 0.3g of sodium dihydrogen phosphate, 0.3g of magnesium sulfate, and 1000ml of water, and mix them to prepare a culture medium. Sterilize at 120°C for 30 minutes, and after cooling, insert 4 pieces of slant strains of black fungus test tubes of 5×5mm, and cultivate them at a rotation speed of 120r / min and a temperature of 25°C for 8 days to prepare black fungus liquid shake flask strains.
[0027] The liquid submerged medium is prepared by mixing 60g of sucrose, 25g of soybean cake powder, 31g of corn steep liquor, 30g of bran, 0.25g of sodium dihydrogen phosphate, 0.25g of magnesium sulfate, 4.1g of soybean oil, and 1000ml of water. %Introduce liquid submerged medium, sterilize at 110°C, cool to room temperature, add black fungus liquid shake flask strains according to 8% of the volume of the seed tank, at a temperature of 25°C, a tank pressure of 0.05MPa, and a stirring speed of 180...
Embodiment 3
[0030] Weigh 100g of sucrose, 10g of soybean cake powder, 10g of corn steep liquor, 10g of bran, 0.06g of diammonium hydrogen phosphate, 0.05g of magnesium sulfate, and 1000ml of water, and mix them to prepare a culture medium. Sterilize at 130°C for 60 minutes, and after cooling, insert 3 pieces of slanted strains of black fungus test tubes of 5×5mm, and cultivate them at a rotation speed of 180r / min and a temperature of 33°C for 10 days to prepare black fungus liquid shake flask strains.
[0031]The liquid submerged medium is prepared by mixing 20g of sucrose, 45g of soybean cake powder, 45g of corn steep liquor, 60g of bran, 0.06g of diammonium hydrogen phosphate, 0.05g of magnesium sulfate, 2g of soybean oil, and 1000ml of water, according to 80% of the volume of the seed tank Insert the liquid submerged culture medium, sterilize at 120°C, cool to room temperature, add black fungus liquid shake flask strains according to 10% of the volume of the seed tank, at a temperature ...
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