Bioelectrode formed by three-dimensional ordered porous oxide modified conductive film and preparation method of bioelectrode

A porous oxide, three-dimensional ordered technology, applied in the field of biosensors, to achieve the effect of improving diffusion ability, good immobilization effect and high sensitivity

Inactive Publication Date: 2013-11-27
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the current research is using single-level pore (mesoporous or macroporous) materials as electrode modification materials, and there are relatively few studies on the combination of mesoporous and macroporous materials for electrochemical sensors.

Method used

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  • Bioelectrode formed by three-dimensional ordered porous oxide modified conductive film and preparation method of bioelectrode
  • Bioelectrode formed by three-dimensional ordered porous oxide modified conductive film and preparation method of bioelectrode
  • Bioelectrode formed by three-dimensional ordered porous oxide modified conductive film and preparation method of bioelectrode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] a. Preparation of conductive film

[0044] After the glass substrate was cleaned and dried, the titanium bonding layer, the gold thin film layer, the titanium bonding layer and the silicon dioxide insulating layer were sequentially deposited by the ion plating method. The thickness of the bonding layer was 5nm, the thickness of the conductive metal layer was 50nm, and the thickness of the insulating layer was 300nm. . That is, a conductive film is obtained;

[0045] b. Preparation of three-dimensional ordered porous oxide modified conductive film

[0046] Preparation of silica sol containing mesoporous structure directing agent, the mass ratio of the mixture is: tetramethyl orthosilicate: P123: ethanol: water: hydrochloric acid = 1: 0.3: 0.1: 0.5: 0.001, fully Hydrolysis reaction. On the conductive film, use mechanical vibration assisted solvent casting process to drip the polystyrene colloidal ball suspension containing 1% by mass on the surface of the conductive fi...

Embodiment 2

[0051] a. Preparation of conductive film

[0052] After the quartz substrate was cleaned and dried, a tantalum bonding layer, a gold thin film layer, a tantalum bonding layer and a silicon dioxide insulating layer were sequentially deposited by sputtering coating method. The thickness of the bonding layer was 25nm, the thickness of the conductive metal layer was 100nm, and the thickness of the insulating layer was 500nm. That is, a conductive film is obtained;

[0053] b. Preparation of three-dimensional ordered porous oxide modified conductive film

[0054] Preparation of silica sol without mesoporous structure directing agent, the mass ratio of the mixture is: tetramethyl orthosilicate: isopropanol: water: sulfuric acid = 1: 10: 50: 0.5, fully hydrolyzed at 10 °C reaction. On the conductive film, the mechanical shock-assisted solvent casting process is used to drip the polystyrene colloidal ball suspension containing 5% by mass on the surface of the conductive film, and m...

Embodiment 3

[0059] a. Preparation of conductive film

[0060] After the silicon substrate with the silicon oxide layer is cleaned and dried, the titanium bonding layer, the platinum thin film layer, the titanium bonding layer and the silicon dioxide insulating layer are sequentially deposited by the evaporation coating method, the thickness of the bonding layer is 50nm, and the thickness of the conductive metal layer is 300nm , The thickness of the insulating layer is 1000nm. That is, a conductive film is obtained;

[0061] b. Preparation of three-dimensional ordered porous oxide modified conductive film

[0062] The alumina sol containing the mesoporous structure directing agent was prepared, and the mass ratio of the mixture was: aluminum nitrate: P123: methanol: water: nitric acid = 1: 0.1: 0.5: 1: 0.1, and a sufficient hydrolysis reaction occurred at 20 °C. On the conductive film, use mechanical vibration assisted solvent casting process to drop the polymethyl methacrylate crystal b...

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Abstract

The invention relates to a bioelectrode formed by a three-dimensional ordered porous oxide modified conductive film and a preparation method of the bioelectrode. The bioelectrode comprises a conductive film electrode which is formed on an insulation substrate, wherein the film electrode is formed on the insulation substrate in a deposition manner through a physical vapor deposition process; a three-dimensional ordered porous oxide layer containing redox proteins is prepared in situ on the surface of the film electrode; the redox proteins are fixed in pores of the three-dimensional ordered porous oxide layer by adopting an adsorption way, a crosslink way or a covalent bonding way. The pores in the three-dimensional ordered porous oxide layer of the bioelectrode are communicated with one another, so that the dispersion of analyte in an ordered porous material modified layer on the surface of the conductive film can be favored, the dispersion capacity of the analyte can be improved, a mesoporous structure which can be matched with different enzyme molecules can be obtained by utilizing different mesoporous structure guiding agents, and the fixation of the enzyme molecules can be favored. The bioelectrode has good biological compatibility, the fixing effect of the redox proteins is good, the dispersion resistance of the analyte is small, and the electron transmission efficiency is high, so that a biological sensor with high sensitivity can be provided.

Description

[0001] technical field [0002] The invention relates to a biosensor, in particular to a bioelectrode using a three-dimensional ordered porous base material modified conductive film as a redox protein carrier and a preparation method thereof. [0003] Background technique [0004] Due to its low production cost, high sensitivity, easy miniaturization, and easy integration with other systems, electrochemical biosensors have attracted more and more attention in the field of electrochemical analysis, and are being used in medical diagnosis, food industry, and environmental testing. be more and more widely used. [0005] Conductive thin films are widely used as electrode materials in the field of electrochemical analysis. Modifying the surface of conductive films has become a common method to improve the immobilization effect of bioactive molecules. To this end, design and seek new carrier materials to further improve the immobilization efficiency of bioactive molecules, r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 唐敏林新华李杰倪琳
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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