Preparation of polyphenol oxidase biosensor and detection of polyphenol oxidase biosensor to pesticide residues

A polyphenol oxidase and sensor technology, which is applied to the determination/inspection of microorganisms, biochemical equipment and methods, and instruments, etc., can solve the problems of analysts' operating level requirements, cumbersome processing methods, long analysis cycles, etc. Catalytic efficiency, low price, low cost effect

Inactive Publication Date: 2013-12-04
JIANGSU UNIV
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Problems solved by technology

Although these traditional methods can quantitatively detect pesticide residues, these methods have problems such as cumbersome pretreatment methods, long analysi

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  • Preparation of polyphenol oxidase biosensor and detection of polyphenol oxidase biosensor to pesticide residues
  • Preparation of polyphenol oxidase biosensor and detection of polyphenol oxidase biosensor to pesticide residues
  • Preparation of polyphenol oxidase biosensor and detection of polyphenol oxidase biosensor to pesticide residues

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Embodiment 1

[0040] The present invention is based on the polyphenol oxidase sensor of polyphenol oxidase modified electrode embedded in carbon nanospheres, the sensor comprises a glassy carbon electrode, the detection end of the glassy carbon electrode is surface-modified with carbon nanospheres and polyphenol oxidase, fumigated in a glutaraldehyde atmosphere The enzyme is firmly fixed on the surface of the glassy carbon electrode.

[0041] The preparation method of the polyphenol oxidase biosensor based on the polyphenol oxidase modified electrode embedded in carbon nanospheres in this embodiment comprises the following steps:

[0042] 1. Preparation of carbon nanospheres: Weigh 6g of glucose with an electronic balance and put it into the lining of a 50ml reaction kettle, accurately pipette 35ml of deionized water into the above reaction kettle, and stir the solution with a glass rod to make the glucose completely Dissolve it, then put it into the reactor, tighten the reactor with a wren...

Embodiment 2

[0055] Detection of Pesticide Residues by Polyphenol Oxidase Biosensor

[0056] The polyphenol oxidase sensor of Example 1 of the present invention is used as the working electrode, the platinum wire electrode is used as the counter electrode, and the saturated dry mercury electrode is used as the reference electrode, and a three-electrode system is established, and the three-electrode system is connected to an electrochemical workstation to detect glyphosate content.

[0057] Working conditions: the buffer used is 0.1M phosphate buffer solution (PBS), and the current response value of the sensor to the same concentration of catechol solution in the range of pH 5.0-8.0. Selected pH = 6.0 as the best value. Under this condition, the current value is large and stable.

[0058] Inhibition time: the polyphenol oxidase sensor is immersed in glyphosate solution and incubated for a period of time, and the enzyme activity is inhibited. The size of the inhibition rate is related to ...

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Abstract

The invention relates to the preparation of a polyphenol oxidase biosensor, which is prepared by adopting carbon nanosphere embedded polyphenol oxidase modified glassy carbon electrode and used for detecting pesticide residues. Polyphenol oxidase is a class of metalloproteinases with wide sources, compared with acetylcholinesterase, butyrylcholinesterase and organophosphorus hydrolase and the like, the polyphenol oxidase has the advantages of wide sources, low price and the like. According to the invention, carbon nanosphere is adopted to embed polyphenol oxidase, and has good biocompatibility, higher mechanical strength and larger specific surface area, so that the fixed quantity of an electrode surface enzyme is improved, and the biological activity of the polyphenol oxidase is maintained to the maximum degree. Therefore, the biological catalytic efficiency of the enzyme, and analysis and detection performance of the biosensor are improved. The polyphenol oxidase biosensor prepared through the method has lower detection limit and an excellent linear range to glyphosate, and has excellent recovery rate to sample detection, meanwhile, has excellent repeatability, reproducibility and stability, and can be used for detecting pesticide residues.

Description

technical field [0001] The invention relates to the preparation of a polyphenol oxidase biosensor and its application in the detection of pesticide residues. Background technique [0002] In recent years, pesticides have been widely used in crop insecticide, weed control and production increase. After the use of pesticides, some pesticide precursors and degradation products remain in plants and soil, seriously endangering environmental safety and human health. Therefore, the detection of pesticide residues has attracted much attention. At present, the detection methods for pesticide residues mainly include liquid chromatography, capillary electrophoresis chromatography and gas chromatography-mass spectrometry. Although these traditional methods can quantitatively detect pesticide residues, these methods have problems such as cumbersome pretreatment methods, long analysis period, expensive equipment, and high requirements for the professional operation level of analysts, so...

Claims

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Application Information

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IPC IPC(8): C12Q1/26G01N27/327G01N27/36
Inventor 蔡健荣周立娜韩恩孙力
Owner JIANGSU UNIV
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