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Method and kit for establishing experimental model of diabetic foot ulcer big mouse infected by Escherichia coli

A technology for diabetic foot ulcers and Escherichia coli, which is applied in the field of establishing an experimental model of diabetic foot ulcers infected with Escherichia coli

Inactive Publication Date: 2014-01-01
SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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  • Application Information

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Problems solved by technology

The results of literature research show that the existing animal models of diabetic foot ulcers mostly simulate a single pathogenic factor (such as peripheral arterial disease, neuropathy or infection), and there is a big gap between the similarity between the animal model and the actual simulation and the research purpose, and the actual The combination of two or more factors is more common in clinical practice

Method used

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  • Method and kit for establishing experimental model of diabetic foot ulcer big mouse infected by Escherichia coli

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Experimental program
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Effect test

Embodiment 1

[0022] Rat type 2 diabetes was induced by high-fat diet combined with streptozotocin, and skin ulcers were induced by deep second-degree scald using a scalding instrument. Quality control bacteria Escherichia coli-ATCC25922 (Escherichia coli, E. ) strain was made into a rat model of diabetic foot ulcer infection.

[0023] 1 Materials and methods

[0024] 1.1 Animals: SD male rats, SPF grade, body weight (180±10) g, provided by the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine.

[0025] 1.2 Main reagents and drugs: Streptozotocin (STZ), purchased from Alexis; high-fat feed (ingredients: basal feed 73%, lard 20%, milk powder 2%, cholesterol 1%, sucrose 4%; Among them, the basic feed formula: barley 15%, wheat 31%, corn 15%, bran 15%, fish meal 6%, soybean meal, oil and salt 18%, the basic feed was purchased from the Chinese People's Liberation Army Naval Medical Research Institute), high The preparation method of the fat feed is as follows: ...

Embodiment 2

[0036] Example 2Western medicine-external application of recombinant bovine basic fibroblast growth factor (trade name: Beifuji) intervention test results

[0037] 1. Methods: Diabetic control group (DM) without treatment; normal ulcer group (N), diabetic ulcer group (DU), coliform control group (ED-C), daily saline dressing; coliform treatment group (ED-C) T), Beifuji 800iu wound dressing was changed daily. The wound area after healing was measured at 0d, 3d, and 10d after dressing change. After healing, 3% sodium pentobarbital was 2ml·Kg. -1 The dose was intraperitoneal injection anesthesia, blood was collected from the abdominal aorta, serum was separated by centrifugation, and skin tissue was taken for examination.

[0038] 2. Data processing: SPSS16.0 was used for statistical analysis, and measurement data were Indicates that the data conforming to normality and homogeneity of variance were analyzed by one-way ANOVA, the components were tested by paired t test, and t...

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Abstract

The invention discloses a method for establishing an experimental model of a diabetic foot ulcer big mouse infected by Escherichia coli. The method includes the following steps that after the big mouse is fed on high-fat diet for eight weeks, streptozotocin is injected, and a diabetic model is manufactured. After the diabetic model is stabilized, II-degree scalding is performed on the skin of the big mouse, and after three days, subcutaneous injection of Escherichia coli suspension liquid is performed in each ulcer position. In addition, the invention further discloses a kit for establishing the experimental model of the diabetic foot ulcer big mouse infected by the Escherichia coli, wherein the kit comprises the Escherichia coli suspension liquid. The diabetic foot ulcer experimental animal model detects variation of biochemical indexes after pharmacological intervention, and a result shows that the model has the infection features of diabetic ulcer and concurrent Gram-negative bacteria. The model has the advantages of being short in ulcer wound surface healing time, sensitive to drug reaction and the like.

Description

technical field [0001] The invention belongs to the field of experimental animal models, in particular to a method and a kit for establishing an experimental model of diabetic foot ulcer rats infected with Escherichia coli. Background technique [0002] With the sharp increase in the incidence of diabetes in my country, the number of patients with diabetic ulcers has also increased. The establishment of a disease model that truly simulates diabetic ulcers at the overall animal level is a medical study to explore the pathogenesis of diabetic ulcers and preclinical pharmacodynamic evaluation. It is of great scientific and clinical significance. [0003] At present, the animal models of diabetic foot ulcers are mostly induced animal models. The results of literature studies show that the existing animal models of diabetic foot ulcers mostly simulate a single pathogenic factor (such as peripheral arterial disease, neuropathy or infection), and there is a large gap between the si...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61K31/7008A01K67/02
CPCY02A50/30
Inventor 柳国斌韩强
Owner SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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