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Preparation method for cadmium hydroxide nano-array capable of adsorbing and extracting DNA

A cadmium hydroxide and nano-array technology, which is applied in the field of preparation of cadmium hydroxide nano-arrays, can solve problems such as limited recycling and unstable nanostructures, and achieve the effects of low cost, stable chemical properties, and wide application prospects

Inactive Publication Date: 2014-01-01
三亚哈尔滨工程大学南海创新发展基地
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the addition of EDTA can dissolve the cadmium hydroxide nanochains and release the trapped DNA fragments, but the small size of the nanostructure is unstable, which limits its recycling in practice

Method used

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  • Preparation method for cadmium hydroxide nano-array capable of adsorbing and extracting DNA
  • Preparation method for cadmium hydroxide nano-array capable of adsorbing and extracting DNA
  • Preparation method for cadmium hydroxide nano-array capable of adsorbing and extracting DNA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Step 1: Take a piece of pure copper and ultrasonically clean the copper with acetone and ethanol;

[0025] Step 2: Mix 20mL of benzene and 20mL of ammonia solution with pH=11.0 in a beaker, and put the cleaned copper piece into the mixture;

[0026] The third step: slowly drop 3.0mL, 1mol / L cadmium nitrate solution into the beaker;

[0027] Step 4: After the dropwise addition, put the beaker into the fume hood. After reacting at room temperature for 24 hours, take out the copper sheet, wash it with distilled water and absolute ethanol several times, and dry it in the air. There is a layer of copper on the surface. The white substance, that is, the cadmium hydroxide nano-array.

Embodiment 2

[0029] Step 1: Take a piece of pure zinc, and use acetone and ethanol to ultrasonically clean the copper sheet;

[0030] Step 2: Mix 20mL of benzene and 20mL of ammonia solution with pH=11.0 in a beaker, and put the cleaned zinc flakes into the mixture;

[0031] The third step: slowly drop 3.0mL, 1mol / L cadmium nitrate solution into the beaker;

[0032] Step 4: After the dropwise addition, put the beaker into the fume hood. After reacting at room temperature for 24 hours, take out the zinc sheet, wash it with distilled water and absolute ethanol several times, and dry it in the air. There is a layer on the surface. The white substance, that is, the cadmium hydroxide nano-array.

Embodiment 3

[0034] Step 1: Take a piece of pure nickel and ultrasonically clean the copper with acetone and ethanol;

[0035] Step 2: Mix 20mL of benzene and 20mL of ammonia solution with pH=11.0 in a beaker, and put the cleaned nickel sheet into the mixture;

[0036] The third step: slowly drop 3.0mL, 1mol / L cadmium nitrate solution into the beaker;

[0037] Step 4: After the dropwise addition, put the beaker into the fume hood, react at room temperature for 24 hours, take out the nickel sheet, wash it with distilled water and absolute ethanol several times, and after drying in the air, there is a layer of nickel on the surface. The white substance, that is, the cadmium hydroxide nano-array.

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Abstract

The invention provides a preparation method for a cadmium hydroxide nano-array capable of adsorbing and extracting DNA. A base metal sheet is placed into a mixed solution system; the mixed solution system is composed of an alkaline solution with the volume of 15-20 ml and the pH of 11, and an organic solution with the volume of 15-20 ml, wherein the organic solution is not soluble in the alkaline solution; a cadmium salt solution with the volume of 2-3 ml and the concentration of 1 mol / L is dripped into the mixed solution system slowly; the mixed solution system is placed for 20-24 hours under a room temperature; therefore the cadmium hydroxide nano-array can be formed on the metal sheet. The preparation method is simple in technology, low in energy consumption and low in cost; the obtained product is stable in chemical property, can be recycled for use, and has wide application prospects in aspects of medical science, biology, materials science and the like.

Description

technical field [0001] The invention relates to a method for preparing a material capable of absorbing and extracting DNA, in particular a method for preparing a cadmium hydroxide nano-array capable of absorbing and extracting DNA. Background technique [0002] DNA is deoxyribonucleic acid. It is a biological macromolecule that can form genetic instructions to guide biological development and the operation of life functions. Its main function is information storage, which can be compared to "the blueprint of life". It contains the instructions necessary to build other compounds in the cell, such as proteins and ribonucleic acid. DNA is a double-helix macromolecule composed of a series of deoxyribonucleotide chains. Deoxyribonucleotides are composed of deoxyribose, phosphoric acid and nitrogenous bases. The bases are divided into four types, namely adenine (A), Guanine (G), Cytosine (C) and Thymine (T). Since the outer phosphate groups of the double helix structure are nega...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01G11/00B01J20/06B01J20/30C07H21/04C07H1/06C12N15/10B82Y30/00B82Y40/00
Inventor 高鹏鲍迪周晓明孙书超王龙强王莹
Owner 三亚哈尔滨工程大学南海创新发展基地
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