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Seed culture medium and culture method for streptomyces aureus

A Streptomyces aureus and culture method technology, applied in the field of biological fermentation, can solve the problems of increasing the procurement cost of production enterprises and seed cultivation costs, increasing the workload of strain researchers, easily affecting the shape and concentration of bacteria, and reducing the cost of procurement and production costs, reducing the amount and type, and the effect of reducing the amount of inoculation

Active Publication Date: 2015-07-15
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1) There are many kinds of organic nitrogen sources in the formula of the seed medium, and more than 4 kinds of organic nitrogen sources need to be added, which increases the purchase cost of the production enterprise and the seed cultivation cost
[0005]2) The seed cultivation time is longer, usually 24-28 hours
[0006]3) In the process of seed cultivation, the quality of the organic nitrogen source is likely to affect the shape and concentration of the bacteria, resulting in a decrease in the quality of the seed solution
[0007]4) Before cultivating Streptomyces aureus seeds, the inoculation amount of the seed culture medium is relatively large, generally 600-800ml / m3, increasing reduce the workload of strain researchers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Configure seed medium 5m 3 : Soybean oil 40L, corn starch 105kg, beer yeast dry residue 145kg, yeast paste 40kg, low-temperature soybean cake powder 60kg, dipotassium hydrogen phosphate 2.0kg, light calcium carbonate 4kg, magnesium sulfate 0.25kg, sodium chloride 4.5kg, amylase 1kg, ammonium sulfate 0.3kg, silicone oil defoamer 200ml.

[0033] The quality of beer yeast dry residue: protein content is 54.3%, phosphorus content is 62.3ug / ml, water content is 4.8%, ash content is 4.1%, and carbohydrate content is 13.7%.

[0034] The seed medium is sterilized, cooled, and pressurized with sterile air. The sterilized seed culture medium has no bacteria contamination, and the sterilized pH is 6.2. Then under flame protection, insert the Streptomyces aureus that has cultivated into the seed tank and carry out seed culture, and the inoculum size is 900ml.

[0035] During the seed cultivation process, the tank pressure is 0.02MPa; 10h before fermentation, the tank tempera...

Embodiment 2

[0037] Configure seed medium 5m 3 : Corn oil 45L, wheat starch 110kg, beer yeast dry residue 135kg, yeast paste 42.5kg, low-temperature soybean cake powder 65kg, dipotassium hydrogen phosphate 1.5kg, light calcium carbonate 4.5kg, magnesium sulfate 0.3kg, sodium chloride 4.0kg , Amylase 1.25kg, ammonium sulfate 0.35kg, silicone oil defoamer 150ml.

[0038] The quality of beer yeast dry residue: protein content is 52.1%, phosphorus content is 68.6ug / ml, water content is 4.2%, ash content is 4.5%, and carbohydrate content is 14.2%.

[0039] The seed medium is sterilized, cooled, and pressurized with sterile air. The sterilized seed medium was free of bacteria contamination, and the sterilized pH was 6.3. Then under flame protection, insert the Streptomyces aureus that has cultivated into the seed tank and carry out seed culture, and the inoculum size is 920ml.

[0040] During the seed cultivation process, the tank pressure is 0.03MPa; 10h before fermentation, the tank t...

Embodiment 3

[0042] Configure seed medium 5m 3 , rapeseed oil 50L, sweet potato starch 100kg, beer yeast dry residue 140kg, yeast paste 45kg, low-temperature soybean cake powder 55kg, dipotassium hydrogen phosphate 1.75kg, light calcium carbonate 5kg, magnesium sulfate 0.2kg, sodium chloride 4.25kg, Amylase 1.5kg, ammonium sulfate 0.25kg, silicone oil defoamer 175ml.

[0043] The quality of beer yeast dry residue: protein content is 56.1%, phosphorus content is 71.4ug / ml, water content is 4.0%, ash content is 4.4%, and carbohydrate content is 14.8%.

[0044] The seed medium is sterilized, cooled, and pressurized with sterile air. The sterilized seed culture medium has no bacteria contamination, and the sterilized pH is 6.4. Then under flame protection, insert the Streptomyces aureus that has cultivated into the seed tank and carry out seed culture, and the inoculum size is 950ml.

[0045] During the seed cultivation process, the tank pressure is 0.04MPa; 10h before fermentation, the tan...

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PUM

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Abstract

The invention relates to a seed culture medium and a culture method of Streptomyces aureus. The seed culture medium contains brewer's yeast dry residue, which is composed of 8-10ml / L of oil, 20-22g / L of starch, and 27-29g / L of brewer's yeast dry residue. L. Yeast extract 8-9g / L, low-temperature soybean cake powder 11-13g / L, dipotassium hydrogen phosphate 0.3-0.4g / L, light calcium carbonate 0.8-1g / L, magnesium sulfate 0.04-0.06g / L, Sodium chloride 0.8-0.9g / L, amylase 0.2-0.3g / L, ammonium sulfate 0.05-0.07g / L and polyether defoamer 0.03-0.04g / L. The present invention can effectively improve the concentration of Streptomyces aureus cells and the quality of cell culture, reduce the inoculum amount at the initial stage of seed culture, shorten the seed culture period, and at the same time reduce the amount of raw and auxiliary materials in seed culture to the greatest extent, reduce production costs, and ensure sufficient supply. Achieve stable and high-quality cultivation of Streptomyces aureus.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, in particular to a seed culture medium and a culture method for reducing the inoculum amount of Streptomyces aureus by using beer yeast dry residue. Background technique [0002] Streptomyces aureus is a type of Streptomyces, which is named for its golden color. Streptomyces aureus has a strong ability to synthesize aureomycin when the temperature is below 30°C; when the temperature exceeds 35°C, it only synthesizes tetracycline. [0003] At present, the organic nitrogen sources commonly used in the seed medium for cultivating Streptomyces aureus in China are mainly corn gluten meal, peanut cake powder, yeast powder and peptone. The main problems that exist: [0004] 1) There are many types of organic nitrogen sources in the formula of the seed medium, and more than 4 kinds of organic nitrogen sources need to be added, which increases the purchase cost of the production enterpri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/49
Inventor 王义任勇
Owner 宁夏泰瑞制药股份有限公司
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