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Double-peak pore-structure polymer support, and preparation method and application thereof

A structural polymer, bimodal pore technology, applied in animal cells, vertebrate cells, tumors/cancer cells, etc., can solve the problems of insufficient pore size accuracy, porosity, poor connectivity between tubes and pores, and uneven pore size distribution. To achieve the effect of improving the connectivity between the tube holes, low cost and simplifying the preparation process

Inactive Publication Date: 2015-05-27
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the biological tissue decellularization method has problems such as limited source, low strength, rapid degradation and immunogenicity; the biggest disadvantage of the one-way temperature gradient freeze-drying method is poor connectivity between tube holes and uneven pore size distribution; and rapid prototyping method Although in The pore structure design method is flexible and controllable, but its pore size accuracy is currently difficult to be less than 100 microns and the porosity is not high enough, so that its advantages in the preparation of honeycomb pore structures are not obvious
Recently, the Chinese patent No. 201010602753.1 discloses a technology of using plant porous tissue as a template to prepare a honeycomb porous scaffold, which can obtain a honeycomb pore structure with a pore size ranging from 10 to 160 microns. Complex, and the honeycomb pores are only connected by a small number of pits in the template, resulting in low connectivity

Method used

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  • Double-peak pore-structure polymer support, and preparation method and application thereof
  • Double-peak pore-structure polymer support, and preparation method and application thereof
  • Double-peak pore-structure polymer support, and preparation method and application thereof

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Effect test

Embodiment 1

[0033] The preparation method of bimodal pore structure polymer scaffold comprises the following steps:

[0034] 1) Preparation of negative porous sodium chloride: the biological template Wisteria is processed into a cylindrical sample, and the sample is impregnated with a saturated NaCl solution and then dried to obtain a NaCl / vine complex, and the operation of impregnating and drying is repeated until NaCl The mass ratio of NaCl to rattan in the rattan compound is 200:100, then the NaCl / rattan compound is dried at 120°C and placed in a muffle furnace, and the temperature is raised from room temperature to 350°C at a rate of 1°C / min And keep it warm for 4 hours, then rise to 790° C. with a heating rate of 10° C. / min, and drop to room temperature with the furnace after 40 minutes of heat preservation, to obtain the negative mold porous sodium chloride of rattan;

[0035]2) Preparation of water-soluble porogen particles: mix powdered sodium chloride and absolute ethanol, ball ...

Embodiment 2

[0040] The preparation method of bimodal pore structure polymer scaffold comprises the following steps:

[0041] 1) Preparation of negative porous sodium chloride: the biological template wisteria is processed into a cylindrical sample, and the sample is impregnated with a saturated NaCl solution and then dried to obtain a NaCl / vine complex, and the operation of impregnating and drying is repeated until the NaCl The mass ratio of NaCl and rattan in the rattan composite is 180:100, and then the NaCl / rattan composite is dried at 120°C and placed in a muffle furnace, and the temperature is raised from room temperature to 350°C at a rate of 2°C / min And keep it warm for 6 hours, then rise to 790° C. with a heating rate of 30° C. / min, drop to room temperature with the furnace after 2 hours of heat preservation, and obtain the negative mold porous sodium chloride of rattan;

[0042] 2) Preparation of water-soluble porogen particles: mix powdered potassium chloride and absolute ethan...

Embodiment 3

[0047] The preparation method of bimodal pore structure polymer scaffold comprises the following steps:

[0048] 1) Preparation of negative porous sodium chloride: the biological template wisteria is processed into a cylindrical sample, and the sample is repeatedly impregnated with saturated NaCl solution and then dried until the NaCl / Then the NaCl / vine composite was dried at 120°C and placed in a muffle furnace. First, the temperature was raised from room temperature to 350°C at a rate of 3°C / min and kept for 2 hours, and then at a rate of 15°C / min. The heating rate is raised to 790°C, and after 20 minutes of heat preservation, it is lowered to room temperature with the furnace, and the negative mold of the rattan is obtained. Porous sodium chloride;

[0049] 2) Preparation of water-soluble porogen particles: mix powdered sucrose and absolute ethanol, ball mill for 12 hours, and sieve to obtain 600-1250 mesh water-soluble porogen particles;

[0050] 3) Preparation of biopol...

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Abstract

A double-peak pore-structure polymer support, and a preparation method and application thereof are disclosed. The preparation method comprises: dipping a biological template wisteria sinensis with a sodium chloride solution and calcining to obtain a negative template porous sodium chloride of wisteria sinensis; preparing waeter-soluble pore forming agent particles, homogenizing the water-soluble pore forming agent particles and a biomacromolecule to form a biomacromolecule / water-soluble pore forming agent particle suspension; immersing the negative template porous sodium chloride of wisteria sinensis into the biomacromolecule / water-soluble pore forming agent particle suspension, successively removing the solvent and the pore forming agent particles / negative template to obtain the double-peak pore-structure polymer support. According to the preparation method, the raw materials are easily available, the technology is simple, and the double-peak pore-structure polymer support is easy to prepare in bulk. The double peak pore structure of the support is composed of micropores with the aperture of 5-20 mu m and tubular pores with the diameter of 50-300 mu m; and the support has the porosity of 85-97%, wide adjustable scopes on aperture distribution and porosity, and high pore connectivity, and is applicable to preparation of tissue regeneration repair supports based on tissue engineering and establishment of tumor cell in-vitro three-dimensional culture models.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to a polymer scaffold with bimodal pore structure and its preparation method and application. Background technique [0002] Porous scaffolds play a crucial role in tissue engineering, which not only determines the shape and size of engineered tissues, but also has an important impact on the survival and growth of cells planted on its surface and inside. For example, a common problem when using tissue engineering techniques to construct engineered tissues is that the cells / tissues at the outer edge of the scaffold are well developed while the central part is imperfect or even necrotic. The recognized reason is that the pore structure is not conducive to the exchange of substances in the scaffold, that is, it is not conducive to the penetration of seed cells into the center of the scaffold, resulting in uneven distribution of cells during inoculation, and it is also not conducive...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J9/26C08L67/04C08L89/00A61L27/56A61L27/18A61L27/22C12N5/09
Inventor 钱军民胥伟军柳雪峰杨腾飞
Owner XI AN JIAOTONG UNIV
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