Preparation method for radix puerariae lactobacillus effervescent tablet
A technology of kudzu root lactic acid bacteria and effervescent tablets, which is applied in the direction of lactobacillus, food preparation, streptococcus/lactococcus, etc., can solve the problems of high storage, transportation and sales costs, and achieve good product stability, easy absorption, and high comprehensive utilization rate Effect
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Embodiment 1
[0022] (1) Preparation of kudzu root juice: 1kg of kudzu root was pulverized to 80 mesh by dry method, and 2.4L tap water was added at 1w / cm 3 Under the ultrasonic power of 20℃, circulate extraction for 30min, centrifuge and filter, and sterilize the filtrate at 80℃ for 30min.
[0023] (2) Fermentation: Add mixed strains of Lactobacillus bulgaricus, Streptococcus thermophilus and Lactobacillus plantarum to Pueraria lobata juice, the ratio of each strain is 1:1:1, the amount of mixed strain is 3cfu, and the fermentation is kept at 37℃ for 72h , The total number of lactic acid bacteria in the fermentation broth reached 11cfu, pH 3.6, 0.5% reducing sugar and 2.3g / L flavonoid content.
[0024] (3) Filtration: After the fermentation, the fermentation broth is centrifuged and filtered at 5000 r / min for 15 minutes to obtain 1.6L of supernatant and 24g of lactic acid bacteria.
[0025] (4) Preparation of kudzu root baking powder: Concentrate the supernatant obtained in step (3), vacuum conce...
Embodiment 2
[0028] The process is the same as in Example 1.
[0029] Step (2): Lactobacillus bulgaricus, Streptococcus thermophilus and Lactobacillus plantarum mixed strains, the ratio of each strain is 1:1:2, the inoculation amount of the mixed strain is 3cfu, the fermentation temperature is 20℃, and the fermentation time It is 120h. The total number of lactic acid bacteria in the fermentation broth reached 8cfu, pH3.9, 0.2% reducing sugar and 2.1g / L flavonoid content.
[0030] Step (3): After the fermentation is finished, the fermentation broth is filtered by centrifugation at 8000 r / min for 5 min to obtain 1.4L of supernatant and 21g of lactic acid bacteria.
[0031] Step (4): vacuum concentration is carried out at a vacuum degree of 0.1 atmosphere to a solid content of 30%, and spray-dried to obtain 0.4 kg of kudzu root baking powder.
[0032] Step (5): The raw and auxiliary materials are composed of 21 g of lactic acid bacteria, 0.4 kg of kudzu root baking powder, 0.004 kg of strawberry fla...
Embodiment 3
[0034] The process is the same as in Example 1.
[0035] Step (2): Lactobacillus bulgaricus, Streptococcus thermophilus and Lactobacillus plantarum mixed strains, the ratio of each strain is 1:1:3, the inoculation amount of the mixed strain is 6cfu, the fermentation temperature is 40℃, and the fermentation time For 6h. The total number of lactic acid bacteria in the fermentation broth reached 8cfu, pH3.8, 1.3% reducing sugar and 2.1g / L flavonoid content.
[0036] Step (3): Centrifugation. Centrifuge at 3 000 r / min for 30 min to obtain 1.8 L of supernatant and 23 g of lactic acid bacteria.
[0037] Step (4): the Pueraria lobata fermentation broth is vacuum concentrated to a solid content of 60% under a vacuum degree of 0.4 atmospheres, and spray-dried to obtain 0.7 kg of Pueraria lobata fermentation powder.
[0038] Step (5): The composition of the raw and auxiliary materials is: 23 g of lactic acid bacteria, 0.7 kg of kudzu root baking powder, 0.56 kg of citric acid, 0.56 kg of baki...
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