Coaxial three-layer high-activity electrospun nanofiber membrane embedded with biological enzymes and its preparation method
A nanofiber membrane and electrospinning technology, which is applied in spinning solution preparation, fiber treatment, rayon manufacturing, etc., can solve the problems of low enzyme reusability, large space mass transfer steric hindrance, and enzyme load limitation, etc. Achieve the effect of improving catalytic efficiency and reusability, increasing enzyme immobilization capacity, and promoting full contact
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Embodiment 1
[0023] a. Disperse 1.0g of polyvinyl alcohol particles into 9ml of deionized water, stir magnetically at room temperature for 1h to fully swell; raise the temperature to 70°C, continue stirring until the solution is clear and transparent, slowly add 0.01gKCl to the above solution, and stir magnetically until completely dissolved;
[0024] b. Dissolve 0.2 mg of active peroxidase in 0.5 ml of phosphate buffer solution with a pH value of 7.0, shake and shake until completely dissolved to obtain a peroxidase dispersion; add the above peroxidase dispersion to 12 ml In the polyvinyl alcohol solution with a concentration of 11% (wt), shake and shake until evenly dispersed, and store at 4°C until use;
[0025] c. Dissolve 1.0g of polystyrene particles in 9ml of tetrahydrofuran solvent, and stir magnetically at room temperature for 1 hour until a uniform, stable, and viscous sol is formed;
[0026] d. Pouring the solutions obtained in steps a to c into the three-nozzle coaxial electro...
Embodiment 2
[0029] a. Disperse 1.0g of polyacrylic acid particles into 6ml of deionized water, stir magnetically at room temperature for 1h to fully swell, raise the temperature to 70°C, continue stirring until the solution is clear and transparent, and slowly add 0.01g of CaCl to the above solution 2 , magnetic stirring until completely dissolved;
[0030] b. Dissolve 0.2 mg of active lipase in 0.5 ml of phosphate buffered saline solution with a pH value of 7.0, shake and shake until completely dissolved to obtain a lipase dispersion; add the above lipase dispersion to 10 ml with a concentration of 10% (wt ) in polyvinyl acid solution, shake well until evenly dispersed, and store at 4°C until use;
[0031] c. Dissolve 1.0g of polymethyl methacrylate particles in 3.5ml of tetrahydrofuran solvent, and stir magnetically at room temperature for 1 hour until a uniform, stable, and viscous sol is formed;
[0032] d. The solutions obtained in steps a~c are respectively poured into the three-no...
Embodiment 3
[0035] a. Disperse 1.0g of polyacrylamide particles into 19ml of deionized water, stir magnetically at room temperature for 1 hour until the solution is clear and transparent, slowly add 0.2g of ethylenediaminetetraacetic acid (EDTA) to the above solution, and stir magnetically until completely dissolved;
[0036] b. Dissolve 0.2 mg of active urease in 0.6 ml of phosphate buffer solution with a pH value of 7.0, oscillate and shake until completely dissolved to obtain a urease dispersion, add the above urease dispersion to 10 ml with a concentration of 10% (wt ) in polyacrylamide solution, shake well until evenly dispersed, and store at 4°C until use;
[0037] c. Dissolve 1.0 g of polyacrylonitrile particles in 12 ml of dimethylformamide solvent, and stir magnetically at room temperature for 1 hour until a uniform, stable, and viscous sol is formed;
[0038] d. Pouring the solutions obtained in steps a to c into the three-nozzle coaxial electrospinning device respectively, usin...
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