Coaxial three-layer high-activity electrospun nanofiber membrane embedded with biological enzymes and its preparation method

A nanofiber membrane and electrospinning technology, which is applied in spinning solution preparation, fiber treatment, rayon manufacturing, etc., can solve the problems of low enzyme reusability, large space mass transfer steric hindrance, and enzyme load limitation, etc. Achieve the effect of improving catalytic efficiency and reusability, increasing enzyme immobilization capacity, and promoting full contact

Inactive Publication Date: 2016-03-16
DALIAN OCEAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of this method can only fix the enzyme monolayer on the surface of the fiber membrane, the enzyme load is limited, and the enzyme falls off during the reaction process, resulting in low enzyme reusability, although the enzyme load and The stability of the immobilized enzyme, but the enzyme activity will be greatly affected
The second method is to blend biological enzymes in nanofibers or use biological enzymes as the core-shell structure of the fiber core. Although the problems of the first method can be overcome, the steric mass transfer of enzymes is relatively large, resulting in enzymes The contact with the substrate is poor, so it is difficult to maintain the activity of the enzyme persistently

Method used

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  • Coaxial three-layer high-activity electrospun nanofiber membrane embedded with biological enzymes and its preparation method
  • Coaxial three-layer high-activity electrospun nanofiber membrane embedded with biological enzymes and its preparation method
  • Coaxial three-layer high-activity electrospun nanofiber membrane embedded with biological enzymes and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] a. Disperse 1.0g of polyvinyl alcohol particles into 9ml of deionized water, stir magnetically at room temperature for 1h to fully swell; raise the temperature to 70°C, continue stirring until the solution is clear and transparent, slowly add 0.01gKCl to the above solution, and stir magnetically until completely dissolved;

[0024] b. Dissolve 0.2 mg of active peroxidase in 0.5 ml of phosphate buffer solution with a pH value of 7.0, shake and shake until completely dissolved to obtain a peroxidase dispersion; add the above peroxidase dispersion to 12 ml In the polyvinyl alcohol solution with a concentration of 11% (wt), shake and shake until evenly dispersed, and store at 4°C until use;

[0025] c. Dissolve 1.0g of polystyrene particles in 9ml of tetrahydrofuran solvent, and stir magnetically at room temperature for 1 hour until a uniform, stable, and viscous sol is formed;

[0026] d. Pouring the solutions obtained in steps a to c into the three-nozzle coaxial electro...

Embodiment 2

[0029] a. Disperse 1.0g of polyacrylic acid particles into 6ml of deionized water, stir magnetically at room temperature for 1h to fully swell, raise the temperature to 70°C, continue stirring until the solution is clear and transparent, and slowly add 0.01g of CaCl to the above solution 2 , magnetic stirring until completely dissolved;

[0030] b. Dissolve 0.2 mg of active lipase in 0.5 ml of phosphate buffered saline solution with a pH value of 7.0, shake and shake until completely dissolved to obtain a lipase dispersion; add the above lipase dispersion to 10 ml with a concentration of 10% (wt ) in polyvinyl acid solution, shake well until evenly dispersed, and store at 4°C until use;

[0031] c. Dissolve 1.0g of polymethyl methacrylate particles in 3.5ml of tetrahydrofuran solvent, and stir magnetically at room temperature for 1 hour until a uniform, stable, and viscous sol is formed;

[0032] d. The solutions obtained in steps a~c are respectively poured into the three-no...

Embodiment 3

[0035] a. Disperse 1.0g of polyacrylamide particles into 19ml of deionized water, stir magnetically at room temperature for 1 hour until the solution is clear and transparent, slowly add 0.2g of ethylenediaminetetraacetic acid (EDTA) to the above solution, and stir magnetically until completely dissolved;

[0036] b. Dissolve 0.2 mg of active urease in 0.6 ml of phosphate buffer solution with a pH value of 7.0, oscillate and shake until completely dissolved to obtain a urease dispersion, add the above urease dispersion to 10 ml with a concentration of 10% (wt ) in polyacrylamide solution, shake well until evenly dispersed, and store at 4°C until use;

[0037] c. Dissolve 1.0 g of polyacrylonitrile particles in 12 ml of dimethylformamide solvent, and stir magnetically at room temperature for 1 hour until a uniform, stable, and viscous sol is formed;

[0038] d. Pouring the solutions obtained in steps a to c into the three-nozzle coaxial electrospinning device respectively, usin...

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Abstract

The invention discloses a coaxial three-layer high-activity bio-enzyme-embedded electrostatic spinning nano-fiber membrane and a preparation method thereof. The coaxial three-way electrostatic spinning technology is used to allow bio-enzyme to be embedded into the sandwich layer of slow release activating agent and porous polymer so as to form the porous nano-fiber membrane. The nano-fiber membrane of a sandwich structure sequentially comprises a slow release activating agent layer (1), an immobilized bio-enzyme layer (2) and a porous polymer layer (3) from inside to outside. By building the structure with communicating ducts, immobilization amount of enzyme is increased, sufficient contact of the enzyme and a substrate is promoted, mass transfer resistance between the enzyme and the substrate is lowered, catalytic efficiency and reusability of the enzyme are increased effectively, environmental sensitivity of the bio-enzyme is lowered, and lasting stability of the bio-enzyme when being applied in complex environments is increased.

Description

technical field [0001] The invention relates to an electrospinning nanofiber membrane and a preparation method thereof, in particular to a coaxial three-layer high-activity embedded biological enzyme with the characteristics of high activity, long-lasting stability and strong environmental broad-spectrum of the immobilized biological enzyme Electrospun nanofibrous membrane and preparation method thereof. Background technique [0002] Bio-enzyme immobilization technology is a technology that uses chemical or physical methods to locate free cells in a limited area to keep them active and reusable. This technology has high bio-enzyme density, long action time, and the ability to resist adverse environments. Strong, less loss of biological enzymes and other advantages. The existing biological enzyme immobilization methods include adsorption method, covalent method, cross-linking method and embedding method. The recovery rate is high, the carrier is easy to get and can be regen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D01D5/00D04H1/728D04H1/4382D01D1/02D01F1/10
Inventor 潘超
Owner DALIAN OCEAN UNIV
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