Cell micro-imaging method, image processing method and imaging analysis system

A technology of microscopic imaging and image processing, applied in image data processing, image enhancement, material analysis using radiation, etc., can solve the problems of neglecting influence, less research on image processing, lack of research on grating and photoresist, etc. To achieve the effect of reducing operation time and cost
CN103760179AInactive Publication Date: 2014-04-30NO 128 HOSPITAL OF HANGZHOU
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NO 128 HOSPITAL OF HANGZHOU
Publication Date
2014-04-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a cell micro-imaging method, an image processing method and an imaging analysis system. The cell micro-imaging method is implemented by using a soft X ray and comprises the following steps of (1) preparing a sample; (2) primarily seeking and selecting a target cell; and (3) imaging the soft X ray. The image processing method is capable of reducing noises, eliminating artifacts and improving the contrast ratio. The imaging analysis system comprises an optical microscope system, a synchrotron radiation device, an image processing system and a display device. The methods and the system provided by the invention have the advantages that the existence and the region environment of a cell are effectively detected, the space scales, the structures and the functions of the cell and a sub-cell are researched, and thus the effective middle information is provided for multiple fields such as pathologies, medicines, agriculture and forestry and foods.
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Description

[0001] technical field

[0002] The invention relates to a method for microscopic imaging of cells by using soft X-rays, an image processing method and an imaging analysis system.

[0003] Background technique

[0004] X-rays discovered by Roentgen in 1895 have the property of strongly penetrating matter and are suitable for imaging human tissues and organs. Its wavelength is shorter, 0.1-1.0nm. However, the interaction between X-rays and matter is very complicated, and it is difficult to manufacture optical components similar to those used in visible light applications, so it is difficult to realize X-ray microscopes. With the birth of the electron collision synchrotron radiation source, special soft X-rays are emitted with a wavelength of 1-10nm. The soft X-rays are focused and projected onto the sample through optical diffraction and optical elements, and then imaged on the image plane.

[0005] The resolution of soft X-ray microscopy has reached 10nm, the principle is...

Claims

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