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Application of enteromorpha polysaccharide in preparation of cosmetic additive

A cosmetic additive, Enteromorpha polysaccharide technology, applied in the application field of Enteromorpha polysaccharide in the preparation of cosmetic additives, to achieve high safety in use, prevent damage, and improve the effect of cell survival

Active Publication Date: 2014-05-07
青岛海大生物集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report that Enteromorpha polysaccharide is used as an additive in the cosmetics industry

Method used

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  • Application of enteromorpha polysaccharide in preparation of cosmetic additive
  • Application of enteromorpha polysaccharide in preparation of cosmetic additive
  • Application of enteromorpha polysaccharide in preparation of cosmetic additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1. Hygroscopic effect test of Enteromorpha polysaccharide

[0024] The preparation method of Enteromorpha polysaccharide of the present invention includes the following steps:

[0025] 1. Enteromorph pretreatment

[0026] Since the outbreak of Enteromorpha has a strong seasonality, it is particularly important to effectively preserve Enteromorpha in the season of a large number of outbreaks for subsequent experiments. The specific processing method is: using the method described in the patent "ZL200910313001.0 Harmless Treatment Method and Equipment of Enteromorpha", the details are as follows: The fresh enteromorpha that has just been salvaged ashore is squeezed with a squeezing dehydration packaging equipment Dehydrate, remove 20-30% of free water, reduce the water content to 60-70%, and reduce the volume to 1 / 4 to 1 / 5 of the original; use a shearing machine to break Entero moss to release the water in the tubular structure of Entero moss The free water is then sub...

Embodiment 2

[0039] Example 2. The moisturizing effect test of Enteromorpha polysaccharides

[0040] Accurately weigh the appropriate amount of Enteromorpha polysaccharide sample prepared by the method described in Example 1, add appropriate amount of distilled water and mix thoroughly, and place it in a desiccator with dry silica gel. The control uses glycerin and hyaluronic acid. After different times Weigh accurately and calculate the moisture retention rate.

[0041] Moisturizing rate (%)=100×W 1 / W 0

[0042] Where W 1 Is the moisture weight after placing, W 0 It is the weight of water before storage, the unit is g.

[0043] It can be seen from the results in Table 2 that as time increases, the moisture retention rate of each substance continues to decrease, and the rate of decline of glycerin is much greater than that of the other two substances. The moisturizing performance is ranked from high to low: hyaluronic acid> Enteromorpha polysaccharides> glycerin. Enteromorpha polysaccharides ca...

Embodiment 3

[0046] Example 3. Cytoprotective effect experiment of Enteromorpha polysaccharides

[0047] In this example, the cytoprotective effect of Enteromorpha polysaccharides on human skin fibroblasts was observed. The conventional MTT method is used for determination. The cell seeding density should be screened before the experiment. Secondly, observe the protective effect of Enteromorpha polysaccharides on human skin fibroblasts: take the appropriate density of cells and spread them in a 96-well plate, and set up a parallel control group and a drug group (low, medium, and high dose). After 48h of culture, change the medium to continue the culture. After the cells entered the logarithmic growth phase, different concentrations of Enteromorpha polysaccharides were added, and the culture was continued for 12 hours. Then remove all the culture medium and continue to culture for 4 hours in a dry environment, then use the MTT method to measure the activity of mitochondria in the cells and ...

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Abstract

The invention provides application of enteromorpha polysaccharide in preparing a cosmetic additive. The enteromorpha polysaccharide can be used as an absorbent, a humectant and a human skin fibroblast protective agent in cosmetics. Experiments and researches discover that enteromorpha polysaccharide has excellent absorption and moistening activities, has a good protective effect on skin cells, and in particular can remarkably improve the cell survival rate under a drying condition, so that the enteromorpha polysaccharide can be applied to cosmetics as humectant and absorbent.

Description

Technical field [0001] The invention belongs to the technical field of Enteromorpha polysaccharides, and particularly relates to the application of Enteromorpha polysaccharides in the preparation of cosmetic additives. Background technique [0002] The skin is a natural barrier of the human body, and moisture absorption and moisturizing are the key factors to maintain the normal function of this barrier. Healthy skin is smooth, soft and elastic. The moisture content in the stratum corneum of the skin is the most important factor in maintaining the softness and elasticity of the skin. Only when moisture is sufficient, cells in skin tissue can carry out normal material metabolism, maintain normal tissue structure and physiological functions, and can resist all adverse factors externally, such as sunlight and chemical stimulation, and can maintain internally The nutrients and moisture in the cells are sufficient to ensure the stability of the environment in the body and the moistur...

Claims

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Application Information

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IPC IPC(8): A61K8/73A61Q19/00
Inventor 单俊伟孙小婷刁红霞宋海妹李秀珍
Owner 青岛海大生物集团股份有限公司
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