Electrochemical sensor for detecting hydrogen peroxide, and preparation method of electrochemical sensor

A sensor and electrochemical technology, applied in the field of electrochemical sensors and their preparation, can solve the problems of harsh measurement environment, great influence of interfering substances, inability to automatically detect, etc., and achieves increased effective electrocatalytic area, low detection limit, and design method. simple effect

Inactive Publication Date: 2014-06-11
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above method is time-consuming as a whole, and the influence of interfering substances is large, so it cannot be detected automatically
On the other hand, although many enzymatic hydrogen peroxide assays have good sensitivity and selectivity, they are demanding on the assay environment and relatively expensive to prepare

Method used

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  • Electrochemical sensor for detecting hydrogen peroxide, and preparation method of electrochemical sensor
  • Electrochemical sensor for detecting hydrogen peroxide, and preparation method of electrochemical sensor
  • Electrochemical sensor for detecting hydrogen peroxide, and preparation method of electrochemical sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1. Preparation and electron microscope characterization of single-armed carbon nanotube-polydopamine-nanosilver composite material and various reference materials

[0050] 1) Dissolve silver nitrate in deionized water to obtain a concentration of 2g. L -1 Silver nitrate aqueous solution;

[0051] Dissolve dopamine in pH 8.5, concentration 10mmol. L -1 Tris-hydrochloric acid buffer solution, prepared 0.1g. L -1 Buffered solution of dopamine;

[0052] Then the silver nitrate solution and the dopamine solution were mixed in equal volumes, and ultrasonicated at a power of 120 W for 10 minutes to obtain a solution containing the polydopamine membrane-silver nanoparticle complex.

[0053] 2) Disperse the single-armed carbon nanotubes into the solution obtained in step 1) until the concentration of the single-armed carbon nanotubes is 2g. L -1 , and ultrasonically disperse for 10 minutes at a power of 120W to obtain a water dispersion system containing carbon n...

Embodiment 2

[0076] Example 2. Preparation of single-armed carbon nanotube-polydopamine-nanosilver modified glassy carbon electrode sensor and cyclic voltammetry characterization

[0077] The first step, glassy carbon electrode pretreatment:

[0078] (a) The glassy carbon electrode is sequentially polished to a mirror surface with 1.0 μm, 0.3 μm and 0.05 μm alumina powder on a polishing cloth;

[0079] (b) ultrasonically clean the glassy carbon electrode twice in secondary water for 2-5 minutes each time, and then dry it;

[0080] (c) Put the glassy carbon electrode at 0.1mol·L -1 In the potassium ferricyanide solution, the cyclic voltammetry scan was performed between -0.4 and +0.7 V until a typical cyclic voltammetry spectrum was obtained, and a clean glassy carbon electrode was obtained.

[0081] The second step is to prepare single-armed carbon nanotube-polydopamine-nanosilver electrochemical sensor:

[0082] 1) Dissolve silver nitrate in deionized water to obtain a concentration of...

Embodiment 3

[0110] Embodiment 3, detect hydrogen peroxide with the electrochemical sensor obtained in embodiment 1 and 2

[0111] The electrochemical sensor obtained in Example 1 is placed in PBS7.0 buffer solution and hydrogen peroxide is continuously added to the solution to obtain the corresponding ampere response curve, wherein the working potential is -0.39V, such as image 3 shown.

[0112] At the same time, the linear relationship between hydrogen peroxide concentration and current is obtained by fitting, such as Figure 4 shown.

[0113] from Figure 4 It can be concluded that the linear range of the electrochemical sensor modified by the optimized single-arm carbon nanotube-polydopamine-nanosilver composite material is 5×10 -5 ~1.75×10 -3 mol. L -1 , the limit of detection for hydrogen peroxide is 0.6×10 -6 mol. L -1 .

[0114] The response current of hydrogen peroxide has a good linear relationship with its concentration, the correlation coefficient R=0.991, and the fi...

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Abstract

The invention discloses an electrochemical sensor for detecting hydrogen peroxide, and a preparation method of the electrochemical sensor. The preparation method comprises the steps of firstly mixing dopamine alkaline solution with silver nitrate solution to form one-armed carbon nanotube-poly dopamine-nano silver functional composite material, and modifying the surface of a glassy carbon electrode by the one-armed carbon nanotube-poly dopamine-nano silver functional composite material to obtain the electrochemical sensor. The electrochemical sensor has good electrochemical catalysis behavior for the reduction of the hydrogen peroxide. The electrochemical sensor is high in sensitivity in hydrogen peroxide detection and good in stability, and has the detection lower limit of 0.6mu mol. L<-1> and the response time shorter than 2s.

Description

technical field [0001] The invention relates to an electrochemical sensor for detecting hydrogen peroxide and a preparation method thereof. Background technique [0002] Hydrogen peroxide (H 2 o 2 ) is an important chemical substance in biological systems, which seriously affects cell function and metabolism, and high concentrations of hydrogen peroxide can even cause cell death. Many enzymes can catalyze substrates to produce hydrogen peroxide, so enzyme activity, enzyme substrate concentration (glucose, lactic acid, glutamic acid and uric acid, etc.) can be detected by measuring the amount of hydrogen peroxide produced (Analytical biochemistry, 2005, 340: 66-73). In addition, hydrogen peroxide is also produced or consumed during many enzymatic reactions, protein accumulation and antigen-antibody recognition. The detection of hydrogen peroxide is also of great significance in the environment, food and other fields. For example, hydrogen peroxide is used as a disinfectan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/30B82Y30/00B82Y15/00B01F3/12
Inventor 林雨青黎琳波胡良璐刘康玉徐亚男李博
Owner CAPITAL NORMAL UNIVERSITY
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