Bradyrhizobium japonicum strain SCAUs36 and application thereof

A brachyrhizobia and soybean technology, which is applied to soybean bradyrhizobium SCAUs36 and its application fields, can solve the problems of scattered planting, small planting area, low yield, etc., and achieves good affinity, strong nitrogen-fixing ability, and resistance to stress. strong effect

Inactive Publication Date: 2014-07-30
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on excellent rhizobium bacteria that match the soybean varieties and ecological environment in the southern soybean producing area, Sichuan soybean producing area.
[0005] Soybean in Sichuan has always been a minor cr

Method used

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  • Bradyrhizobium japonicum strain SCAUs36 and application thereof
  • Bradyrhizobium japonicum strain SCAUs36 and application thereof
  • Bradyrhizobium japonicum strain SCAUs36 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The isolation, purification and preservation of embodiment 1 soybean bradyrhizobium SCAUs36

[0016] From the soybeans planted on the purple soil in the hilly area of ​​Luxian County, Luzhou City, Sichuan Province, select the large and full red root nodules on the taproots of robust plants, scrub the root nodules clean, pick off part of the root bark, dry them with paper and put them on the In a small tube filled with anhydrous calcium chloride and covered with absorbent cotton. After soaking the collected root nodules in sterile water for imbibition in the laboratory, soak in 95% ethanol for 30 seconds to eliminate surface tension, then disinfect the surface with 0.1% (m / v) mercury chloride for 5 minutes, and then rinse with sterile water 6 times, in the case of aseptic operation, a single root nodule was clipped and then added with Congo red YAM medium (mannitol 10g, yeast powder 0.8g, KH 2 PO 4 0.25g, MgSO 4 .7H 2 O0.2g, CaCl 2 .6H 2 O0.1g, NaCl0.1g, 1% (m / v) s...

Embodiment 2

[0019] Back grafting and matching test of embodiment 2 rhizobia

[0020] The backgrafting test of rhizobium was carried out by hydroponics. The soybean variety used in the test was "Nandou 12", the variety with the largest planting area in Sichuan. ) and harvested after 46 days of planting. After successful back-grafting with "Nandou 12", the compatibility test with other main soybean varieties was carried out, and the hydroponics method was also carried out. The soybean variety used in the test: another main soybean variety "Gongxuan 1" for intercropping ", the main spring soybean varieties "Nandou 8" and "Chuandou 14". After being cultivated in the above-mentioned light chamber for 1 week, they were cultured under normal light and temperature conditions, and harvested 41 days after planting. Regular supplement of sterile micro-nitrogen nutrient solution, formula of micro-nitrogen nutrient solution: 0.46g calcium sulfate, 0.075g potassium chloride, 0.06g magnesium sulfate, ...

Embodiment 3

[0030] Example 3 Stress resistance of soybean bradyrhizobium SCAUs36

[0031] The stress resistance of soybean bradyrhizobium SCAUs36 was mainly determined by acid and alkali tolerance, salt tolerance and growth greenhouse range. The YMA medium was used as the basal medium, and the YMA plate cultured at pH 7 and 28°C for 7 days was used as the positive control. The above-mentioned YMA slant culture of Bradyrhizobium soybean SCAUs36 was scraped and washed with sterile water for use. The point inoculation method was adopted and repeated 3 times. YMA medium was used as the basal medium for the determination of acid and alkali resistance, and the pH value was adjusted with HCl and NaOH. The pH value was 4.0, 5.0, 6.0, 8.0, 9.0, 10.0, 11.0, and 12.0. Salt tolerance determination also uses YMA medium as the base medium, and the strains are spot-inoculated on a plate containing NaCl, and the mass volume fraction of NaCl is 1%, 2%, 3%, 4% and 5%. Plates for acid and alkali resistan...

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Abstract

The invention discloses a Bradyrhizobium japonicum SCAUs36 and application thereof. The strain is separated and purified from fresh soybean root nodules. The strain is collected to China Center for Type Culture Collection of Wuhan University on March 14th, 2014, the collection number is CCTCC NO.M2014082, and the classification designation is Bradyrhizobium japonicum. The strain is applied to production of Sichuan soybeans. The Bradyrhizobium japonicum SCAUs36 is a favorable broad-spectrum Rhizobium japonicum strain with high symbiotic fixation capacity, wide application range for Sichuan soybean species, IAA (indoleacetic acid) secretion capacity, capacities for dissolving inorganic phosphorus and organic phosphorus and high stress tolerance. The strain has favorable matching affinity with Sichuan dominant bean species, and can enhance the soybean yield by more than 31% in different ecological regions where no nitrogen fertilizer is applied and the SCAUs36 is inoculated, which is much different from the regions where the SCAUs36 is not inoculated.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of Bradyrhizobium soybean SCAUs36 and its application. Background technique [0002] Chemical nitrogen fertilizers have made great contributions to increasing crop yields, but they have high production costs, low utilization rates, and pollute the ecological environment. Biological nitrogen fixation has the advantages of low cost, large amount of nitrogen fixation, continuous nitrogen fixation process, and no pollution, which is conducive to the protection of the ecological environment and the sustainable development of agriculture, and is an effective means of saving costs and increasing efficiency in agricultural production. Great efforts are needed to develop environmentally friendly biological nitrogen fixation technologies in food production. The rhizobia-legume nitrogen fixation system is the most efficient in biological nitrogen fixation. Artificial inoculation of ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P21/00A01G31/00C12R1/41
Inventor 徐开未陈远学陈强张小平陈翠平蒋攀
Owner SICHUAN AGRI UNIV
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