Preparation method of microcarrier-cell compound with induction activity and application of compound

Abstract

The invention discloses a preparation method of a microcarrier-cell compound with induction activity and an application of the compound. The preparation method comprises the following steps: respectively using decalcified bone matrix particles and acellular dermal matrix micro particles to culture bone marrow mesenchymal stem cells for 7-21 days, differentiating into osteoblasts and fibroblasts to obtain an osteoblast-particle compound and a fibroblast-particle compound, and then, mixing evenly. The activity of the seed cells of the prepared product are higher, the product can be used for preparing repair material for treating lacunar bone defect and segmental bone defect, the in vivo repair effect is better, and the in vivo repair effect of tissue-engineered bone for more than two times; supporting cell components are added in the construction process of the tissue-engineered bone to promote the bone formation effect of bone parenchymal cells in bone formation in vivo, the new bone strength, the matrix component content and the calcification extent can be improved for 1.5-2 times, the utilization rate of the seed cells is obviously improved, the type of the bone repair seed cells is enriched, and the bone defect repair effect is greatly improved.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and specifically relates to a preparation method and application of a microcarrier-cell complex with inducible activity. Background technique [0002] At present, in the process of tissue engineering bone construction, during the use of seed cells, many steps such as digestion and collection of seed cells are required to destroy the extracellular matrix when the seed cells are combined with the scaffold material, which will undoubtedly lead to the activity of the seed cells used. In addition, a single type of seed cell-osteoblast is mostly used, because it is the main and direct cell component of osteogenesis. [0003] The reason for this problem is that the scaffold materials used for tissue engineering bone construction are mostly bulky due to the need to provide sufficient bone function replacement, so their specific surface area, effective adhesion area and other cell culture-related param...

AI Technical Summary

Problems solved by technology

[0002] At present, in the process of tissue engineering bone construction, during the use of seed cells, many steps such as digestion and collection of seed cells are required to destroy the extracellular matrix when the seed cells are combined with the scaffold material, which will undoubtedly lead to the activity of the seed cells used. In addition, a single type of seed cell-osteoblast is mostly used, because it is the main and direct cell component of osteogenesis

[0003] The reason for this problem is that the scaffold materials used for tissue engineering bone construction are mostly bulky due to the need to provide sufficient bone function replacement, so their specific surface area, effective adhesion area and other cell culture-related parameters are relatively low. , which is not conducive to the in vitro cultivation of seed cells; and the scaffolds currently used for in vitro cultivation of seed cells, such as microcarriers, cannot be used for in vivo transplantation due to various reasons such as poor degradability, poor biocompatibility, and high cost, which in turn leads to the loss of seed cells. During the construction of tissue engineered bone, repeated digestion and separation steps are required

In addition, in the construction of bone tissue engineering, the current selection of seed cells is mainly cultured and osteogenic induced BMSCs (bone marrow mesenchymal stem cells), and the osteoblasts produced by them can directly complete the osteogenic activity in vivo. Therefore, it becomes the first choice for seed cells. However, related studies have shown that in the process of bone tissue formation, in addition to parenchymal cells such as osteoblasts, supporting cells such as fibroblasts and endothelial cells also play an irreplaceable role. Experiments have also confirmed that the addition of fibroblasts in bone tissue engineering construction can significantly promote the quality and speed of osteogenesis, but there is still a lack of feasible methods for the combined use of osteoblasts and fibroblasts. Respectively compound with scaffold material before transplantation for further in vivo transplantation

[0004] In the current tissue engineering bone construction strategy, due to repeated cell digestion and separation, the seed cell activity and cell utilization rate are significantly reduced, resulting in low construction efficiency; in addition, the in vivo tissue engineering bone construction using only osteoblasts The repair effect of bone defect is relatively limited and can be improved in a large range, and the combined use of fibroblasts is one of the optional ways to obtain this effect

Images