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Preparation method of amylose inclusion complexation carrier

A technology of inclusion and complexation and amylose, which is applied in the field of preparation of amylose inclusion and complexation carrier, can solve the problems of low production cost and high cost of amylose preparation, achieve high production efficiency, improve inclusion and complexation synergy, important social and economic benefits

Active Publication Date: 2014-08-27
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for preparing an amylose inclusion-complexing carrier with low production cost and high efficiency for the disadvantage of high amylose preparation cost in the prior art

Method used

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  • Preparation method of amylose inclusion complexation carrier

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Embodiment 1

[0021] In the first step, the wheat starch is prepared into starch milk with a mass percentage concentration of 5% by disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution, and the pH is adjusted to 4.5, and it is placed in an airtight container at 95° C. for 30 minutes to gelatinize;

[0022] In the second step, the gelatinized liquid obtained in step (1) is cooled, and pullulanase (OPTIMAX L-1000, Genencor Bioengineering Co., Ltd.) is added in an amount of 10u of pullulanase per gram of dry starch, at 60 Stir the reaction at ℃ for 3 hours;

[0023] The third step is to adjust the pH to 6.0, add medium temperature α-amylase (CLARASE L, Genencor Bioengineering Co., Ltd.), the dosage is 1u of medium temperature α-amylase per gram of dry starch, stir and react at 50°C for 10 minutes , adjust the pH to 2.5 and keep it for 10 minutes to inactivate the enzyme; add iodine-potassium iodide mixed solution prepared with water, control the concentration of iodine in th...

Embodiment 2

[0034] In the first step, the cornstarch is mixed with disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution to make starch milk with a concentration of 12% by mass, and adjust the pH to 6.5, and place it in a closed container at 96°C for 60 minutes to gelatinize;

[0035] The second step is to cool the gelatinized liquid obtained in step (1), add isoamylase (Pseudomonas sp, Sigma-Aldrich company), the dosage is 30u of isoamylase per gram of dry starch, and stir and react at 50°C for 1 hour;

[0036]The third step is to adjust the pH to 5.5, add high temperature resistant α-amylase (Liquozyme Supra, Novozymes company), the dosage is to add 10u of high temperature resistant α-amylase per gram of dry starch, stir and react at 95°C for 1 minute, adjust the pH to 2.7 and keep for 12 minutes to inactivate the enzyme; add iodine-potassium iodide mixed solution prepared with water, control the concentration of iodine in the mixed solution to 1mg / mL, and control the c...

Embodiment 3

[0039] In the first step, the waxy cornstarch is mixed with disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution to prepare starch milk with a mass percentage concentration of 8%, and adjust the pH to 5.0, and place it in a closed container at 99°C for 40 minutes to gelatinize;

[0040] In the second step, the gelatinized liquid obtained in step (1) is cooled, and pullulanase (OPTIMAX L-1000, Genencor Bioengineering Co., Ltd.) is added in an amount of 20u per gram of dry starch. Stir the reaction at ℃ for 2 hours;

[0041] The third step is to adjust the pH to 7.0, add high temperature resistant α-amylase (Liquozyme Supra, Novozymes company), the dosage is 5u of high temperature resistant α-amylase per gram of dry starch, stir and react at 110°C for 2 minutes, adjust the pH to 3.0 and keep it for 15 minutes to inactivate the enzyme; add iodine-potassium iodide mixed solution prepared with water, control the concentration of iodine in the mixed solution to 2m...

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Abstract

The invention discloses a preparation method of an amylose inclusion complexation carrier. The method comprises the following steps: preparing a starch raw material into starch milk by a disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution, adjusting the pH to 4.5-6.5, putting the starch milk in an enclosed container for gelatinization; after the gelatinization, cooling the gelatinized liquid, adding a debranching enzyme, stirring and reacting at 45-65 DEG C for 1-3 hours; adjusting the pH to 5.5-7.0, adding alpha-amylase, stirring and reacting at 45-110 DEG C for 1-10 min, performing enzyme deactivation; adding an iodine-potassium iodide mixed solution prepared by water, controlling the concentration of the potassium iodide in the mixed solution to be 5-20 mg / mL; performing thermal insulation and stirring at 60-80 DEG C for 10-20 min; performing neutralization to obtain a pH of 6.0-6.5, drying, and crushing. The amylose carrier prepared by the invention can realize inclusion complexation of substances of essence perfume, aliphatic acid, inorganic micromolecules, and the like, and has wide applications in industries of food, medicine, cosmetics, chemical engineering, and the like.

Description

technical field [0001] The invention relates to a production method of modified starch, in particular to a method for preparing a starch inclusion and complexation carrier capable of loading functional factors by using biotechnology and adding an inducer. Background technique [0002] Starch is a renewable carbohydrate composed of two polysaccharide molecules, amylose and amylopectin. Amylose is a linear molecule connected by α-1,4 glycosidic bonds; amylopectin is a branched molecule formed by the polymerization of D-glucopyranose, and its linear part is α-1,4 glycosidic bond, while the branch point is α-1,6 glycosidic bond. [0003] Amylose in aqueous solution is generally in the form of random coils. When there are appropriate guest molecules in the solution, amylose molecules can form a single helical chain, and the entire helix has a cylindrical cavity structure. Similar to the structure of cyclodextrin, the inner cavity of the single helical chain of amylose is hydrop...

Claims

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Application Information

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IPC IPC(8): C12P19/16C12P19/14C12P19/04
Inventor 罗志刚程玮玮扶雄王新阁
Owner SOUTH CHINA UNIV OF TECH
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