Wide-spectrum plant drought-resistant agent and application thereof to drought resisting of crops

A drought-resisting agent and plant technology, applied in the field of plant resistance to drought and adversity, can solve problems such as limited application, and achieve the effects of reducing chemical residues, using less amount and having a wide range of sources.

Inactive Publication Date: 2014-10-15
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, many of these microorganisms are pathogenic bacteria, such as marine Vibrio, which can infect mammals and cause sepsis, thus limiting the application of this type of microorganisms

Method used

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  • Wide-spectrum plant drought-resistant agent and application thereof to drought resisting of crops
  • Wide-spectrum plant drought-resistant agent and application thereof to drought resisting of crops
  • Wide-spectrum plant drought-resistant agent and application thereof to drought resisting of crops

Examples

Experimental program
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preparation example Construction

[0038] The preparation process of algin oligosaccharides: the algin lyase used has high enzyme activity and stability, and the degradation process of algin oligosaccharides is as follows: prepare a 1%-5% mass concentration of algin polysaccharide solution, mix algin polysaccharide with Equal volumes of fermented crude enzyme liquids were mixed, stirred continuously at 45°C-55°C, and degraded for 8h-10h. Add ethanol to the degraded enzymolysis solution, adjust the final mass concentration of ethanol to 70%-85%, let it stand for 12h-24h, and centrifuge at 8000-10000 rpm to remove unreacted polysaccharides and impurities, and obtain the supernatant as alginate The oligosaccharide mixture solution is concentrated by rotary evaporation to remove ethanol, and the concentrated sugar solution is lyophilized and vacuum-dried to obtain alginate oligosaccharide powder.

[0039] The preparation process of algin oligosaccharide derivatives: the algin lyase used has high enzyme activity and...

Embodiment 1

[0040] Embodiment 1 Commonly used this plant anti-drought agent water preparation

[0041] The plant drought resistance agent is made into water, emulsifiable concentrate, soluble liquid, water emulsion, microemulsion, suspending agent by adding solvent and auxiliary agent; it is made into powder, soluble powder, wettable powder, granule by adding known carrier and auxiliary agent. Water-dispersible granules, microcapsules, tablets.

[0042] This example introduces a commonly used water preparation ratio, but the content of the present invention is not limited thereto: 1 g of alginate oligosaccharides with a degree of polymerization of 2-6 is dissolved in 1 kg of water to make 0.1% of the plant's drought-resistant water agent. Wherein the algin oligosaccharide content can be detected by the following method:

[0043] The quality of algin oligosaccharides was determined by liquid chromatography-mass spectrometry equipped with electrospray ionization source (ESI). The chromat...

Embodiment 2

[0044] tested as figure 1 As shown, the degree of polymerization of algin oligosaccharides is 2-10, and its main degree of polymerization is 2-6. Embodiment 2 uses this plant anti-drought agent to improve the activity of tobacco stress-resistance-related enzymes

[0045] Use the water agent of this drought resistance agent (0.3% algin oligosaccharide, 1.5% MgCl 2 , 2%KH 2 PO 4 , and the balance is water), the tobacco was treated with root irrigation or foliage spraying, and each tobacco plant was applied with 10mL of the drought-resistant agent water agent, and samples were taken after seven days to detect the stress-resistant enzyme activity by spectrophotometry (Chen Yafei et al., Functions of oligochitosan induced protein kinase in tobacco mosaic virus resistance and pathogenesis related proteins in tobacco.Plant Physiology and Biochemistry47(2009)724–731; Yin Heng Chitooligosaccharides Induced Antibacterial Sclerotinia and Molecular Mechanisms in Rape The treatment gro...

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Abstract

The invention discloses a wide-spectrum plant drought-resistant agent, and the active composition of the agent is alginate oligosaccharide or a alginate oligosaccharide derivative. When drought comes, the drought-resistant agent containing alginate oligosaccharide or the alginate oligosaccharide derivative is capable of improving the drought resistance of plants, the drought resistant effect reaches 50%-90%, specifically the survival rate of the above plant is improved by 50%-90%, the water content in the plant is improved by 60%-80%, and synthesis of various enzymes and secondary metabolites correlated to plant drought resistance is increased by 75%-90%. Additionally, the oligosaccharide also helps to induce expression of genes correlated to drought resistance. The agent taking alginate oligosaccharide or the alginate oligosaccharide derivative as the main composition has the advantages of being safe, nontoxic, low in cost, small in usage amount, environment-friendly and the like. By using the plant drought-resistant agent to perform spraying, seed soaking, root wetting, root pouring and other processing on crops, the drought resistant capability of the plant is substantially improved, and the output and the quality of crops are substantially increased.

Description

technical field [0001] The present invention relates to the drought stress resistance of plants, in particular to a drought-resistant agent for plants with algin oligosaccharides or algin oligosaccharide derivatives as the main active substance and its application. Background technique [0002] Drought and other adversity stresses are extremely harmful to the growth of crops. The losses caused by plant adversity in my country are tens of billions of yuan each year. At present, these adversity stresses are usually dealt with by improving field management techniques or using chemical agricultural preparations. However, the excessive use of chemical agricultural preparations has gradually exposed problems such as low utilization rate, environmental pollution, and food safety, which has caused great negative impacts on agricultural production and the ecological environment, and has attracted widespread attention worldwide. Especially in recent years, there have been frequent acc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01P21/00
Inventor 杜昱光刘航赵小明尹恒王文霞刘启顺王梦雨
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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