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A kind of sperm selection slide and selection method based on microtube fluid circuit board

A technology of sperm and microtubules, applied in the field of sperm selection, can solve the problems of difficult control of sperm quality, peroxidative damage of sperm DNA, and decline of sperm quality, so as to avoid the decline of sperm quality, easy to control sperm quality, and good screening effect Effect

Active Publication Date: 2016-08-24
ZHEJIANG CELLPRO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The recovery rate of high-quality sperm is increased after density gradient centrifugation, but it is easy to cause peroxidative damage to sperm DNA and reduce the quality of sperm
The invention patent with the Chinese patent announcement number CN102352305 discloses the use of microfluidic core technology to detect and screen sperm. Buffer is added to the buffer sample pool, and the high-motility sperm in the sample pool is brought to the sample collection pool through the buffer flow to achieve the screening and collection of high-motility sperm. However, the patented technology of this invention is relatively complicated. Mucus is adsorbed, and the buffer is required to passively flush the sperm flow to the sample collection pool. The sperm is not actively flowing, and the quality of the sperm is not easy to control

Method used

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  • A kind of sperm selection slide and selection method based on microtube fluid circuit board
  • A kind of sperm selection slide and selection method based on microtube fluid circuit board

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Such as figure 1 The shown preferred glass slide for sperm based on a microtube fluid path plate includes a sample pool 1 and a sperm collection pool 2. A layer of straight tube type microtube channels 3 is provided between the sample pool 1 and the sperm collection pool 2. Two waste pools 4 are also provided on the sperm preferred glass slide, and a layer of straight tube type microtubule channels 3 is provided between the waste pool 4 and the sperm collection pool 2. The inner diameter of the microtube channels 3 is 10 to 200 microns (such as human The sperm can be set to 35 microns), the microtube channel 3 is 50-100 microns from the bottom of the sample pool 1, the sperm collection pool 2 and the waste pool 4.

Embodiment 2

[0016] It is basically the same as Example 1, except that there are 1 or 3 waste pools, and the microtubule channels are curved tubes.

Embodiment 3

[0018] A method of using the sperm-optimizing glass slides of Example 1 or Example 2 to optimize sperm. First, add hyaluronic acid in the sperm collection tank, the surface of the hyaluronic acid is 50-100 microns from the bottom of the sperm collection tank, and then transfer the semen into In the sample pool, fill the sample pool with sperm culture solution at the same time. The sperm culture solution contains sodium chloride with a concentration of 0.1 mol / L, potassium dihydrogen phosphate with a concentration of 0.4 mmol / L, and a concentration of 2.7 mmol / L. Glucose, potassium chloride with a concentration of 4.7mmol / L, magnesium sulfate heptahydrate with a concentration of 0.2mmol / L, calcium chloride dihydrate with a concentration of 2mmol / L, Hepes-Na with a concentration of 0.02mol / L, 4mmol / L sodium bicarbonate, 0.3mmol / L sodium pyruvate, 0.34% by volume of C3H5O3Na, and 0.002% by mass of Phenol Red; put the sperm-preferred glass slide at 37℃, Keep at a constant temperatu...

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Abstract

The invention discloses a sperm optimal selection slide based on a microtubule liquid path board. The sperm optimal selection slide comprises a sample tank and a sperm collection tank, a layer of microtubule passage is arranged between the sample tank and the sperm collection tank, one to three waste tanks are further arranged on the sperm optimal selection slide, a layer of microtubule passage is arranged between the waste tank and the sperm collection tank, the inner diameter of the microtubule passage is 10-200 micron, and the distances from the microtubule passages to the tank bottoms of the sample tank, the sperm collection tank and the waste tank are 50-100 micrometer. Sperms actively flow into the sperm collecting tank along with a sperm nutrient solution by using the initiative attracting of the sperm nutrient solution to sperms and the running of the sperm nutrient solution on the microtubule liquid path board, high-activity sperms are adhered by using the adhering effect of the hyaloplasm liquid of the sperm collecting tank, sperms with poor quality continue to flow into the waste tank along with the running of the sperm nutrient solution, and sperms inside the sperm collecting tank are highly energetic.

Description

Technical field [0001] The invention relates to sperm optimization, in particular to a sperm optimization glass slide based on a microtubule liquid path plate and a method for sperm optimization. Background technique [0002] Sperm motility is one of the key factors for conception. Semen with high sperm motility has a relatively high number of sperm with strong motility, so the probability of conception is also high. The currently widely used sperm optimization techniques include upstream techniques, density gradient centrifugation techniques and other optimization techniques. The upstream technology is designed using the principle of sperm's own vitality and upward swimming. In the upstream process, the sperm can be separated from certain endogenous sperm inhibitory substances in the seminal plasma, such as anti-sperm antibodies, various types of cells, etc., and make the sperm in the The extracorporeal activity time can be extended, but the upstream technology has relatively l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12N5/076
CPCC12M47/04C12N5/0612C12N2509/00C12N2533/80
Inventor 曾桥薛志刚
Owner ZHEJIANG CELLPRO BIOTECH