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Method and device for determination of cigarette smoke benzo[alpha]pyrene by on-line solid phase extraction

A technology of cigarette smoke and solid-phase extraction column, which is applied in the field of analytical chemistry, can solve the problems of large solvent consumption, troublesome off-line solid-phase extraction purification operation, and the introduction of many error factors, and achieve the effect of improving analytical sensitivity

Inactive Publication Date: 2014-11-05
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The off-line solid-phase extraction purification operation adopted is cumbersome, introduces many error factors, and consumes a large amount of solvent; therefore, it is necessary to establish a more convenient and environmentally friendly method for the determination of benzo[α]pyrene in flue gas

Method used

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  • Method and device for determination of cigarette smoke benzo[alpha]pyrene by on-line solid phase extraction
  • Method and device for determination of cigarette smoke benzo[alpha]pyrene by on-line solid phase extraction

Examples

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Effect test

Embodiment 1

[0034] The method for determining benzo[α]pyrene in cigarette smoke by on-line solid-phase extraction, using the above-mentioned device, comprises the following steps:

[0035] (1) Smoke capture: Smoke capture is based on the conditions of GB / T 19609-2004, and the cigarette sticks are balanced for 48 hours at a relative humidity of 55% and a temperature of 20°C; the interval between each puff is 60s, and the smoke The time is 2s, the suction volume is 34.7mL, and 20 domestic flue-cured cigarettes are smoked, and the total particulate matter in the smoke is captured with a 92mm Cambridge filter;

[0036] (2) Sample extraction: Put the Cambridge filter piece that has captured the total particle phase of mainstream smoke into a 150mL conical flask with a stopper, accurately add 1mL of the third-level internal standard solution with a pipette, and accurately add 40mL of ring Hexane, ultrasonic vibration for 40min, ultrasonic frequency 60%, cooled to room temperature, for chromatog...

Embodiment 2

[0042] The method for determining benzo[α]pyrene in cigarette smoke by on-line solid-phase extraction, using the above-mentioned device, comprises the following steps:

[0043] (1) Smoke capture: Smoke capture is based on the conditions of GB / T 19609-2004. The cigarette sticks are balanced for 48 hours at a relative humidity of 60% and a temperature of 22°C; the interval between each puff is 60s. The time is 2s, the suction volume is 35mL, and 20 foreign mixed cigarettes are sucked, and the total particulate matter in the smoke is captured with a 44mm Cambridge filter;

[0044] (2) Sample extraction: Put the Cambridge filter disc with the total particle phase of mainstream smoke into a 150mL conical flask with a stopper, accurately add 1mL of the third-level internal standard solution with a pipette, and accurately add 40mL of the secondary Chloromethane, ultrasonically oscillated for 30 minutes, ultrasonic frequency 60%, cooled to room temperature, for chromatographic analysi...

Embodiment 3

[0050] The method for determining benzo[α]pyrene in cigarette smoke by on-line solid-phase extraction, using the above-mentioned device, comprises the following steps:

[0051] (1) Smoke capture: Smoke capture is based on the conditions of GB / T 19609-2004. The cigarette sticks are balanced for 48 hours at a relative humidity of 65% and a temperature of 24°C; the interval between each puff is 60s. The time is 2s, the suction volume is 35.3mL, and 20 foreign mixed cigarettes are sucked, and the total particulate matter in the smoke is captured with a 92mm Cambridge filter;

[0052] (2) Sample extraction: Put the Cambridge filter with the total particle phase of mainstream smoke into a 150mL Erlenmeyer flask with a stopper, accurately add 1mL of the third-level internal standard solution with a pipette, and accurately add 40mL of acetone , ultrasonically oscillating for 50 minutes at an ultrasonic frequency of 60%, cooling to room temperature for chromatographic analysis; wherein...

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Abstract

Belonging to the technical field of analytical chemistry, the invention discloses a method and device for detection of cigarette smoke benzo[alpha]pyrene. Including sample preparation and analysis, the detection method of benzo[alpha]pyrene specifically comprises: conducting trapping by a filter disc, adding an organic solvent to conduct ultrasonic shaking so as to obtain an analysis sample; separating the sample by a liquid chromatogram, cutting the needed outflow component into a gas chromatogram, and performing adsorption by a solid phase extraction column; and then starting GC-MS, and bringing the to-be-tested component into a capillary separation column to perform analysis. The device is a gas chromatograph-mass spectrometer with an on-line solid phase extraction purification function, wherein the solvent treatment between the liquid chromatogram and the gas chromatogram is solid phase extraction. With the device provided by the invention, efficient purification of the sample and larger volume sample injection of the gas chromatogram can be realized. For ultra low content samples, components subjected to liquid phase separation can be accumulated repeatedly to an ideal analysis amount and then transferred to the gas chromatogram for analysis. While achieving efficient purification of the sample, the analysis sensitivity is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, and in particular relates to a method and a device for detecting benzo[α]pyrene in cigarette smoke. Background technique [0002] At present, it is an indisputable fact that smoking is harmful to health. Research at home and abroad shows that the impact of smoking on health mainly comes from the chemical substances released by tobacco combustion. The harmful components in the particle phase of cigarette smoke mainly include tobacco-specific nitrosamines, heterogeneous cyclic amines, naphthylamines, other nitrosamines, fused-ring aromatic hydrocarbons such as benzo[α]pyrene, monophenols and polyphenols (such as catechol), solid-phase free radicals, polonium 210, uranium, nickel, cadmium, etc. Among the many harmful components of smoke, benzo[α]pyrene has the highest carcinogenic activity. [0003] The content of benzo[α]pyrene in cigarette smoke is very low, and the smoke sample mat...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
Inventor 申钦鹏杨光宇孔维松缪明明刘春波张涛张凤梅朱瑞芝何沛王昆淼刘志华陈永宽
Owner CHINA TOBACCO YUNNAN IND
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