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A preparation method of anti-protein adsorption capillary electrophoresis covalently bonded coating column

A technology of capillary electrophoresis and anti-protein adsorption, which is applied in separation methods, chemical instruments and methods, and material analysis by electromagnetic means, and can solve the problem of poor quality of anti-protein adsorption capillary electrophoresis covalently bonded coating columns and preparation process Problems such as complex process and high toxicity in the production process, to achieve the effect of green production process, simple process equipment, and overcome coating quality problems

Active Publication Date: 2017-04-19
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a simplified method for preparing an anti-protein adsorption capillary electrophoresis covalently bonded coating column to solve the problem of complex preparation process, harsh operating conditions, high toxicity in the production process, low production efficiency, high production cost, and the Problems with Poor Quality Anti-Protein Adsorption Capillary Electrophoresis Covalently Bonded Coated Columns

Method used

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  • A preparation method of anti-protein adsorption capillary electrophoresis covalently bonded coating column

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Experimental program
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Embodiment 1

[0021] At 1°C, wash a new bare quartz capillary column with an aqueous solution of diazo resin (molecular weight: 400) at a concentration of 0.01 mg / ml for 5 minutes, and then rinse it with distilled water for 1 minute. Then rinse with 0.01 mg / ml sodium polystyrene sulfonate (molecular weight: 5000) aqueous solution for 5 minutes, and then rinse with distilled water for 1 minute, thus completing one cycle of electrostatic self-assembly. After assembling one cycle, the capillary was blown dry with compressed air, and exposed under a UV lamp with a wavelength of 193nm, and the exposure dose was 5mJ / cm 2 , to form an anti-protein adsorption covalently bonded coating column with a diazo resin-sodium polystyrene sulfonate 2-layer composite structure. Since the covalent cross-linking reaction between the silanol on the inner wall of the quartz capillary and the coating is shielded, the covalently bonded coating column has good anti-protein adsorption performance.

Embodiment 2

[0023] At 50°C, a new bare quartz capillary column was first rinsed with an aqueous solution of diazo resin (molecular weight: 100,000) at a concentration of 1000 mg / ml for 5 minutes, and then rinsed with distilled water for 1 minute. Then rinse with an aqueous solution of sodium polystyrene sulfonate (molecular weight: 600,000) at a concentration of 1000 mg / ml for 5 minutes, and then rinse with distilled water for 1 minute, thus completing an electrostatic self-assembly cycle. After repeated assembly for 6 cycles, the capillary was blown dry with compressed air, and exposed to a UV lamp with a wavelength of 400nm, and the exposure dose was 20000mJ / cm 2 , to form an anti-protein adsorption covalently bonded coating column with a diazo resin-sodium polystyrene sulfonate 12-layer composite structure. Since the covalent cross-linking reaction between the silanol on the inner wall of the quartz capillary and the coating is shielded, the covalently bonded coating column has good an...

Embodiment 3

[0025] At 20°C, a new bare quartz capillary column was first rinsed with an aqueous solution of diazo resin (molecular weight 750) at a concentration of 0.1 mg / ml for 5 minutes, and then rinsed with distilled water for 1 minute. Then rinse with 0.1 mg / ml sodium polystyrene sulfonate (molecular weight: 10,000) aqueous solution for 5 minutes, and then rinse with distilled water for 1 minute, thus completing one cycle of electrostatic self-assembly. After repeated assembly for 5 cycles, the capillary was blown dry with compressed air and exposed to a UV lamp with a wavelength of 248nm at a dose of 30mJ / cm 2 , the anti-protein adsorption covalent bonding coating column with diazo resin-sodium polystyrene sulfonate 10-layer composite structure can be formed. Since the covalent cross-linking reaction between the silanol on the inner wall of the quartz capillary and the coating is shielded, the covalently bonded coating column has good anti-protein adsorption performance.

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Abstract

The invention provides a preparation method of a protein-adsorption-resistant covalently-bonded capillary electrophoresis coating coated column. By virtue of electrostatic self-assembly and photo-curing crosslinking reaction of poly(sodium-p-styrenesulfonate) and photopolymer diazoresin, a covalently-bonded protein-adsorption-resistant coating is built on the inner wall of a quartz capillary column. The capillary electrophoresis coating coated column has good protein-adsorption-resistant property, and compared with an uncoated quartz capillary column, the coated column has the advantage that the protein separating property is enhanced by over one time. According to the preparation method, the technological process is simple, the production efficiency is high, the production cost is low, the repeatability is good, nontoxic and environmental-friendly effects are achieved, and large-scale production is facilitated; the covalently-bonded capillary electrophoresis coating coated column has good chemical stability and protein-adsorption-resistant property and is applicable to electrophoretic separation detection and purification of biological samples of protein, glycoprotein, peptide chain, hormone, enzyme and the like.

Description

technical field [0001] The present invention relates to the preparation method of anti-protein adsorption capillary electrophoresis covalently bonded coating column, exactly a kind of electrostatic self-assembly and photocuring cross-linking reaction of sodium polystyrene sulfonate and photosensitive polymer diazo resin in quartz A method for constructing a covalently bonded anti-protein adsorption coating on the inner wall of a capillary column. Background technique [0002] With the development of capillary electrophoresis technology in various fields of life science research, the coating performance requirements for anti-protein adsorption capillary electrophoresis coating columns are also getting higher and higher. The ideal coating should not only maintain the modification effect on the capillary wall, but also inhibit The adsorption of protein on the tube wall will not fall off and cause pollution to the combined equipment. Compared with non-covalently bonded coatings...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D57/02G01N27/447
Inventor 丛海林于冰焦明明
Owner QINGDAO UNIV
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