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Kit and method for rapidly detecting thiocyanate in milk and milk product

A technology of thiocyanate and dairy products, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, which can solve the problems of long detection time, high price, and complicated processing. , to achieve the effect of short detection time, low detection cost and good reproducibility

Inactive Publication Date: 2014-11-26
BEIJING ZHIYUNDA TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ion chromatography is a detection method recommended by the Ministry of Health. This method has complex sample pretreatment and a long detection time; capillary electrophoresis saves solvents and has diversified separation modes. However, ion chromatography and capillary electrophoresis are expensive, and both require professional technicians to operate, which is not conducive to the use and promotion in small enterprises, and is not easy to carry and do rapid detection; The spectrophotometric method mostly uses the ferric ammonium sulfate method or the isonicotinic acid-pyrazolone method. The detection limits of these two methods are relatively high, and the accuracy for low-concentration thiocyanate is low; milk and dairy products contain Various nutrients such as amino acids, proteins, fats, etc., the matrix is ​​relatively complex, which has a great impact on the detection results, so it is not suitable for determination by potentiometric titration
Moreover, the existing detection methods all need pretreatment and use instruments for measurement, which cannot meet the requirements of rapid detection

Method used

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  • Kit and method for rapidly detecting thiocyanate in milk and milk product
  • Kit and method for rapidly detecting thiocyanate in milk and milk product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Qualitative detection of sodium thiocyanate content in a milk sample

[0023] Three kinds of commercially available milk were respectively selected and tested according to the above detection steps, and the detection results are shown in Table 1.

[0024] (1) Sample treatment: Take 1ml of liquid milk in a 10ml colorimetric tube, dilute to the 10ml mark with distilled water, cover and mix well;

[0025] (2) Determination: Take 1ml of the processed sample solution in a 1.5ml centrifuge tube, add 4 drops of sodium thiocyanate reagent A, 2 drops of sodium thiocyanate reagent B, cover and mix well, add 2 drops of sulfur in 1 minute Sodium cyanate reagent C, mix well, observe the color change of the solution within 5 minutes;

[0026] (3) Judgment of results: If the solution turns red, it means that the milk contains sodium thiocyanate, and the sample is a positive sample; if there is no change in the color of the solution, it means that no sodium thiocyanate i...

Embodiment 2

[0030] Embodiment 2: qualitative detection sodium thiocyanate content in milk powder sample

[0031] Three commercially available milk powders were respectively selected and tested according to the above qualitative detection steps, and the detection results are shown in Table 2.

[0032] (1) Sample treatment: Weigh 0.5 grams of milk powder into a 10ml colorimetric tube, dilute to the 10ml mark with distilled water, shake the cap fully and mix well;

[0033] (2) Determination: Take 1ml of the processed sample solution in a 1.5ml centrifuge tube, add 4 drops of sodium thiocyanate reagent A, 2 drops of sodium thiocyanate reagent B, cover and mix well, add 2 drops of sulfur in 1 minute Sodium cyanate reagent C, mix well, observe the color change of the solution within 5 minutes;

[0034] (3) Judgment of results: If the solution turns red, it means that the milk contains sodium thiocyanate, and the sample is a positive sample; if there is no change in the color of the solution, i...

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Abstract

The invention provides a kit and a method for rapidly detecting thiocyanate in milk and a milk product, which can rapidly detect the content of thiocyanate in the milk and the milk product, is high in sensitivity, short in detection time and good in repeatability and can meet the requirement of enterprises and supervision and law enforcing departments such as quality supervision, and industry and commerce on in-situ rapid detection. The kit comprises a sodium thiocyanate reagent A, a sodium thiocyanate reagent B, and a sodium thiocyanate reagent C; the sodium thiocyanate reagent A is anhydrous monopotassium phosphate solution with the mass fraction of 5 to 30 percent; the sodium thiocyanate reagent B is chloramine T solution with the mass fraction of 1 to 10 percent; the sodium thiocyanate reagent C is a mixed solution of isonicotinic acid, barbituric acid and sodium hydroxide, the mass fraction of isonicotinic acid is 1 to 10 percent, the mass fraction of barbituric acid is 1 to 5 percent, and the mass fraction of sodium hydroxide is 1 to 10 percent.

Description

technical field [0001] The invention relates to a kit and a method for rapidly detecting thiocyanate in milk and dairy products. Background technique [0002] Thiocyanate is a chemical raw material often used in various industries such as medicine, printing and dyeing, and is generally white crystal or powder. Adding an appropriate amount of thiocyanate to milk can effectively inhibit bacteria, keep fresh and prolong the shelf life of milk. It is usually added in the form of sodium thiocyanate in food. In 2008, the Ministry of Health issued the "List of Non-edible Substances and Food Additives That May Be Added Illegally in Food (the first batch)" clearly stipulating that sodium thiocyanate in milk and dairy products is an illegally added substance. [0003] At present, there is no national standard or industry standard for the detection of sodium thiocyanate in food, and the main methods for determination include ion chromatography, capillary electrophoresis, spectrophotom...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 叶升锋桑华春刘亚谢艳红
Owner BEIJING ZHIYUNDA TECH CO LTD
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