Preparation method of high-purity lysophosphatidylcholine

Abstract

The invention discloses a preparation method of lysophosphatidylcholine. The method comprises the steps: in a calcium ion solution, firstly adding phosphatidylcholine and cholate, stirring, next adding phospholipase A2, carrying out heat preservation, carrying out a hydrolysis reaction to obtain lysophosphatidylcholine, carrying out acetone precipitation, and separating out the product. With addition of the cholate, phosphatidylcholine emulsion droplets become smaller and thinner, the reaction solution becomes diluted, the reactants are completely hydrolyzed in about 5 hours, and the reaction time can be significantly shortened, wherein the phosphatidylcholine concentration reaches up to 30-40%, and the efficiency of converting phosphatidylcholine into lysophosphatidylcholine can still reach 100%. In addition, method by-products such as glycerophosphorylcholine and non-hydrolytic phosphatidylcholine are significantly reduced compared with the prior art. The produced lysophosphatidylcholine has high purity, multi-time purification steps can be omitted, and thus the production cycle can be shortened, and the production cost can be substantially reduced.

Description

technical field [0001] The invention relates to a method for preparing high-purity lysophosphatidylcholine, in particular to a method for preparing high-purity lysophosphatidylcholine from lecithin. Background technique [0002] Lysophosphatidylcholine is a metabolite of lecithin, also known as lysophosphatidylcholine, which can be used as a food additive and emulsifier in the food industry and cosmetics industry. High-purity lysophosphatidylcholine can be used as a drug intermediate in medicine It is a safe and non-toxic drug carrier. For example, in patent CN100536851, as a carrier of valproic acid, its conjugate can be used as a class of excellent antiepileptic drugs, and its preparation usually uses phospholipase A2 to promote the hydrolysis of phosphatidylcholine to produce lysophosphatidylcholine. [0003] It has been known since the beginning of the twentieth century that phosphatidylcholine can be converted to lysophosphatidylcholine by enzymatic hydrolysis. In 193...

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Problems solved by technology

Chinese patent CN1210560 expects to improve the hydrolysis effect by adding monoglyceride, but because the reaction temperature is too high and the reaction time is too long, there are still many by-products in the reactant, and the addition of monoglyceride is very large, which is phosphatidylcholine 5 times the amount), the cost is very high

[0004] The phosphatidylcholine hydrolysis method disclosed in the prior art still has some disadvantages, such as low product purity, conversion efficiency of phosphatidylcholine raw material not exceeding 60-70%, glycerol phosphorylcholine and unhydrolyzed phosphatidylcholine When the by-products are high, especially when the phosphatidylcholine with high concentration (concentration is 30-40%) is hydrolyzed, due to the high concentration and the viscosity of the reactant, it takes up to 96 hours of reaction time to convert into lysophosphatidylcholine The efficiency is lower, and the proportion of by-products is higher. In order to obtain high-purity final products, multiple purifications are required to remove by-products, which limits its industrial production.

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