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Vaccine composition and preparation method and application thereof

A technology of vaccine composition and bursa, which is applied in the field of vaccine composition, can solve the problems of increasing the burden of manpower and material resources on chicken farms, increasing susceptibility of chickens to diseases, and limited protection of new epidemic strains, etc. The effect of production time, shortening reproduction time, and increasing virus titer

Active Publication Date: 2015-01-14
LUOYANG HUIZHONG BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to causing the death of some chicks, the disease often causes immunosuppression in recovered chickens, resulting in failure of immunization and increasing the susceptibility of chicks to many diseases such as Newcastle disease
[0006] There are many varieties of commercialized products on the market at home and abroad, but they are all single traditional vaccines. Repeated use of vaccines, especially inactivated vaccines, not only increases the manpower and material resources of the chicken farm, but also causes the stress response of chickens to be caught many times. Can affect performance and increase susceptibility of flocks to disease
Coupled with the continuous mutation of H9 subtype avian influenza strains in recent years, although a variety of vaccines have been introduced, the development of the epidemic is still uncontrollable
[0007] At the same time, H9 subtype avian influenza strains have mutated in recent years, and previously isolated strains have limited protection against new epidemic strains

Method used

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  • Vaccine composition and preparation method and application thereof
  • Vaccine composition and preparation method and application thereof
  • Vaccine composition and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 H9 subtype avian influenza virus SZ strain cross-protection test to existing epidemic strains

[0032] 1. Method

[0033] Four viruses were selected from the isolates from 2012 to 2013, and the immune efficacy of inactivated vaccine of SZ strain was compared. See Table 1 below for specific information on the strains.

[0034] Table 1 Detailed information of 4 isolates of avian influenza

[0035] Strain name

separation time

Separation location

A019

2012

Jiangsu

A055

2012

Shandong

A089

2013

Shandong

A119

2013

Anhui

[0036] (1) Preparation of bird flu virus SZ strain seedling virus: the virus seed is properly diluted with sterilized physiological saline (10 -3 or 10 -4 ), the allantoic cavity was inoculated with 10-day-old SPF chicken embryos, 0.1ml per embryo. Select chicken embryos that died 24 to 48 hours after inoculation and had obvious disease scars, and harvested chic...

Embodiment 2

[0055] Example 2 Construction of E.coli BL21(DE3) / pColdⅢ-VP2 Escherichia coli Genetic Engineering Bacteria

[0056] 1. Experimental materials

[0057] Plasmid extraction kit was purchased from Tiangen Biotech; T4DNA Ligase was purchased from BioLab; EcoR1, Sal1 restriction endonuclease, and pColdⅢ_DH5α strain were purchased from TaKaRa; agarose gel recovery kit was purchased from Tianze Biology, and other reagents were of analytical grade .

[0058] 2. Experimental steps

[0059] 2.1 Preparation of VP2 cDNA

[0060] 2.1.1 Extraction of total RNA

[0061] The brief process of total RNA extraction is as follows: grind the bursa of SPF chickens infected with the supervirulent Chengdu strain of chicken infectious bursal disease virus with a grinder. Take 200 μL of disease material and add TE (10mM Tris, 1mM EDTA, pH8.0) to 500μL, add 5μL of proteinase K and 50μL of 10% (W / V) sodium dodecylsulfonate (SDS), and bathe in water at 56°C for 3 hours . Add an equal volume of phenol...

Embodiment 3

[0086] Embodiment 3 The preparation of the production seed poison of the present invention

[0087] (1) Virus seed preparation for the production of chicken Newcastle disease virus La Sota strain: the virus seed is properly diluted with sterilized physiological saline (10 -4 or 10 -5), the allantoic cavity was inoculated with 10-day-old SPF chicken embryos, 0.1ml per embryo. Select chicken embryos that died 72 to 120 hours after inoculation and had obvious lesion scars, and harvest chicken embryo fluid (allantoic fluid and amniotic fluid) respectively, and put them in sterilized containers. The chicken embryo fluid tested for sterility and whose agglutination value for 1% chicken erythrocytes is not lower than 1:256 (micro method) is mixed, quantitatively dispensed into sterile ampoules, and frozen for storage. Indicate the date of harvest, generation of poisonous seeds, etc.

[0088] (2) virus seed preparation for chicken infectious bronchitis virus M41 strain production: ...

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Abstract

The invention provides a vaccine composition, comprising an immune amount of newcastle disease virus antigen, infectious bronchitis virus antigen, H9 subtype avian influenza virus antigen and infectious bursal virus antigen. Meanwhile, the invention also provides a preparation method and application of the vaccine composition. The vaccine composition provided by the invention is capable of effectively preventing the newcastle disease, infectious bronchitis, H9 subtype avian influenza and infectious bursal disease. Meanwhile, the labor intensity of people can be greatly reduced, the risk of aviculture farmers is reduced, and the public food safety is ensured.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a vaccine composition, its preparation method and application. Background technique [0002] Newcastle disease (ND) has a high morbidity and fatality rate, and it occurs frequently all over the world. Once it breaks out, it will bring a devastating blow to the poultry industry. It is a major infectious disease that endangers the poultry industry. OIE lists it as a Category A disease. [0003] Chicken infectious bronchitis (Infectious Bronchitis, IB) infection can lead to slow growth of chicks and broilers, decreased egg production and decreased egg quality of laying hens. The OIE lists it as a Category B disease. [0004] Avian influenza (H9 subtype) (Avian Infectious H9subtype) is caused by avian influenza virus (Avian Infectious Virus), mainly prevalent in animals and humans, an acute and highly contagious disease with high mortality and wid...

Claims

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Application Information

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IPC IPC(8): A61K39/215A61P31/14A61P31/16A61K39/145A61K39/12A61K39/17
Inventor 张许科孙进忠郭丽霞
Owner LUOYANG HUIZHONG BIOTECH
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