Method for separating and purifying 4,8-DHT from pecan exocarp and application of 4,8-DHT
A 8-DHT, separation and purification technology, applied in the separation/purification, application, botany equipment and methods of carbonyl compounds, etc., to achieve the effect of inhibiting plant seed germination, less impurities, and inhibiting seedling growth
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Embodiment 1
[0013] The method of separating and purifying 4,8-DHT from the pecan exocarp, crushing the air-dried pecan exocarp, passing through a 45-60 mesh sieve to obtain 10g of hickory exocarp powder, and putting the hickory exocarp powder into ultrasonic extraction Add 180-200ml of distilled water to the kettle, and the method of ultrasonic generation is: it excites the ultrasonic sound, radially vibrates the columnar transducer, the ultrasonic power is 100W, the ultrasonic frequency is 28kHz, and the extraction method is ultrasonic once every 2min, each time 4min , under the condition of air-lift stirring at 25°C, ultrasonically extract for 30-45min, filter to obtain the extract of pecan exocarp; under the condition of pressure of 450-500Pa, the extract of pecan exocarp is concentrated under reduced pressure to the original volume 1 / 10, and then passed through X-5 macroporous resin, with volume ratios of 10:0, 9:1, 8:2, 7:3, 6:4, 5:5, 4:6, 3:7 , 2:8, 1:9, 0:10 mixed solution of water...
Embodiment 2
[0015] The 4,8-dihydroxyl-1-tetralone of Example 1 is configured into 0.1mM, 0.5mM treatment solution for the germination inhibition experiment of lettuce, radish, cucumber, onion, wheat seeds, and each 100 seeds of the test species are taken , placed evenly in a germination box with two layers of filter paper, the size is 15×20 cm, and 10 ml of different concentrations of treatment solution (the control group is deionized water), and each treatment set 3 replicates. The culture conditions were light cycle 25°C, 12h; dark cycle 15°C, 12h. Seed germination is based on the radicle breaking through the seed coat. The number of lettuce seeds germinated on the fourth and seventh days, the number of radish seeds germinated on the fourth and tenth days, and the cucumber seeds and wheat seeds on the fourth and eighth days The number of germination, the number of germination of onion seeds was recorded on the sixth day and the twelfth day. The final result: 0.1mM 4,8-DHT treatment can...
Embodiment 3
[0017] The 4,8-dihydroxy-1-tetralone of Example 1 is configured into 0.1mM and 0.5mM treatment solutions for the inhibition experiment on the growth of lettuce, radish, cucumber, onion, and wheat seedlings, and the radicle is taken to break through the seed coat 100 seeds each were placed evenly in a germination box with a size of 15 × 20 cm covered with two layers of filter paper, and 10 ml of different concentrations of treatment solutions (deionized water for the control group) were added, and 3 replicates were set for each treatment. The culture conditions were light cycle 25°C, 12h; dark cycle 15°C, 12h. Every other day, 5 seeds were randomly selected from the germination box to measure the length of radicle, germ and fresh weight, and the measurements were taken 6 times in total. 0.1mM and 0.5 mM 4,8-DHT treatment can significantly reduce the radicle growth of test species (p<0.05). 0.1mM 4,8-DHT treatment can inhibit the growth of lettuce germ by about 45%, the growth ...
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