Carrier for microarray biochip, preparation method of carrier and microarray biochip

A biochip and microarray technology, applied in the field of microarray biochips and carriers of microarray biochips, can solve the problem of lack of universal functional groups for immobilized biomolecules, difficult liquid injection and flow control in microfluidic pipelines, and high obstacles. The wide application of molecular materials to achieve the effect of avoiding individual differences, good reactivity, and convenient functional modification

Pending Publication Date: 2015-02-18
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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Problems solved by technology

[0004] However, the surface of high-molecular polymer materials has two obvious disadvantages: one is chemically inert and lacks general functional groups for immobilizing biomolecules; the other is highly hydrophobic, which makes it difficult to perform liquid injection and flow control
The existing technology adopts different methods for activation modification for different polymer materials, and the lack of general functionalization methods has become a major bottleneck hindering the wide application of polymer materials in the field of biochips

Method used

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  • Carrier for microarray biochip, preparation method of carrier and microarray biochip
  • Carrier for microarray biochip, preparation method of carrier and microarray biochip
  • Carrier for microarray biochip, preparation method of carrier and microarray biochip

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preparation example Construction

[0041] The present invention provides a method for preparing a carrier for a microarray biochip described in the above technical scheme, comprising the following steps:

[0042] Applying a photolithography method to coat a plurality of masks on the surface of the polymer substrate, and the plurality of masks are distributed in an array on the surface of the polymer substrate;

[0043] physical vapor deposition of a silicon dioxide film on the polymer substrate covered with a mask to obtain a carrier precursor;

[0044] The mask on the carrier precursor is peeled off to obtain a carrier for microarray biochips.

[0045] In the present invention, preferably, the polymer material is first molded, injected and cut to obtain a polymer material of suitable size and shape. In the present invention, the material, size, and shape of the polymer substrate are consistent with those of the polymer substrate in the above technical solution, and will not be repeated here.

[0046] After o...

Embodiment 1

[0077] (1) Cyclic olefin copolymer (purchased from Topas, USA) is processed into a rectangular substrate of 76 mm × 26 mm by a plastic thermocompression molding machine;

[0078] (2) Use NH with a volume ratio of 1:1:5 4 OH:H 2 o 2 :H 2 O mixed solution Wash the cycloolefin copolymer substrate obtained in step (1) at 80° C. for 15 minutes. Dry the substrate under vacuum at 110°C for 1 hour with nitrogen flow;

[0079] (3) Rotate at a speed of 4000 rpm for 30 s, spin-coat a thin layer of positive photoresist Shipley 1813 (purchased from MicroChem, USA) onto the substrate obtained in step (2), and dry at 80° C. for 2 min. A Karl-Suss lithography machine (purchased from Karl Suss, Germany) was used for photolithography, and developed by MF 319 developer (purchased from MicroChem, USA);

[0080] (4) Deposit a silicon dioxide film on the substrate obtained in step (3) using a Kurt Lesker PVD 75 radio frequency magnetron sputtering system (purchased from Kurt J.Lesker, USA): th...

Embodiment 2

[0085] (1) Polycarbonate (purchased from Sinopharm Chemicals) was processed into a rectangular substrate of 76mm×26mm by a plastic thermoforming machine;

[0086] (2) The substrate obtained in step (1) was ultrasonically cleaned with ethanol and deionized water for 5 minutes and 10 minutes in sequence, and the substrate was dried under nitrogen flow and then vacuum-dried at 80° C. for 0.5 hours;

[0087] (3) Rotate at a speed of 4000 rpm for 30 s, spin-coat a thin layer of positive photoresist Shipley 1813 (purchased from MicroChem, USA) onto the substrate obtained in step (2), and dry at 80° C. for 2 min. A Karl-Suss lithography machine (purchased from Karl Suss, Germany) was used for photolithography, and developed by MF 319 developer (purchased from MicroChem, USA);

[0088] (4) Use a vacuum ion sputtering coating machine (Sputter Coating SCD005, purchased from Finland BAL-TEC company) to sputter deposit a silicon dioxide film on the substrate obtained in step (3): the work...

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Abstract

The invention provides a carrier for a microarray biochip. The carrier comprises a polymer substrate and multiple silicon dioxide films arranged on the polymer substrate. The multiple silicon dioxide films are distributed in arrays on the polymer substrate. According to the carrier, a polymeric material is used as the substrate, and multiple silicon dioxide films are distributed in arrays on the polymer substrate. Thus, the surface of the polymeric material carrier can be processed by a general activation method. Silylanization treatment, for instance, is used in the embodiment of the invention so as to avoid individual difference of the polymeric material carrier activation treatment and simplify carrier surface activation treatment. In addition, the surface of the carrier provided by the invention has good reactivity, which is convenient for further functional modification and immobilization of biological information molecules.

Description

technical field [0001] The invention relates to the technical field of biochips, in particular to a carrier of a microarray biochip, a preparation method thereof and a microarray biochip. Background technique [0002] Biochip technology is one of the most far-reaching major scientific and technological advances since the mid-1990s. It is a highly intersecting new technology integrating microelectronics, biology, physics, chemistry, and computer science. It has great basic research value and Obvious prospects for industrialization. This technology can immobilize a large number of probes on the support at the same time, and can detect and analyze a large number of biomolecules in turn, so as to make up for the shortcomings of traditional nucleic acid imprinting hybridization technology, such as complexity, low degree of automation, low detection target molecular weight, and low throughput. Due to its high-throughput, large-scale and integrated features, biochip technology is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C40B40/06C40B40/10
Inventor 冯淼田敬东王璐王丽娜
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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