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Microbial feed additive

A technology for microbial feed and additives, applied in the field of agricultural microbial feed additives and microbial feed additives, can solve the problem of insurmountable intestinal microecological disturbance, residual nutritional stress of drugs, increase of susceptible and resistant strains, and difficulties in disease control and treatment. and other problems, to achieve the effect of promoting sustainable development, good anti-pathogenic bacteria, improving egg production and feed conversion rate

Inactive Publication Date: 2015-03-11
BIOLOGY INST OF HEBEI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term abuse of antibiotics has led to low animal resistance and an increase in susceptible and resistant strains, making disease control and treatment more difficult, and antibiotics cannot overcome a series of problems such as intestinal microecological disorders, drug residues, and nutritional stress. Make livestock and poultry products, especially meat, eggs, milk and other foods, have great potential safety hazards, causing great harm to people's health

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1 Saccharomyces cerevisiae liquid-solid two-phase fermentation

[0028] Saccharomyces cerevisiae adopts a liquid-solid two-phase culture process, and the liquid phase medium is a GPY medium; the solid phase culture adopts a conventional medium and a medium announced by the present invention for fermentation.

[0029] Commonly used solid medium is: wheat bran: gluten bran = 80 parts: 20 parts, other components and proportions are added to the solid medium, and the total amount of solid matrix components is 100 g, and 4.0 g of starch and 2.0 g of glucose are added. , ammonium sulfate 1.0 g, potassium dihydrogen phosphate 0.5 g, and the pH value is natural; these ingredients are dissolved in 150 mL of water, then stirred evenly with the solid matrix, and steam sterilized.

[0030]The solid medium disclosed by the present invention is: wheat bran: Ganoderma lucidum bran = 80 parts: 20 parts. Add other components and proportions in the solid medium, according to ...

Embodiment 2~7

[0040] Bacillus subtilis medium: peptone 12 g / L, starch 10 g / L, beef extract 5 g / L, yeast extract 3 g / L, KH 2 PO 4 0.5 g / L, K 2 HPO 4 0.5 g / L, MgSO 4 0.5 g / L, CaCO 3 3 g / L, 1 L of water, pH 7.0±0.2, autoclave at 121°C for 30 min.

[0041] Clostridium butyricum medium: tryptone 0.3 g / L, yeast extract 0.5 g / L, peptone 0.4 g / L, L-cysteine ​​hydrochloride 0.3 g / L, sodium chloride 1.0 g / L, K H 2 PO 4 1.0 g / L, MgSO 4 0.5 g / L, 2 drops of resazurin indicator with a mass fraction of 0.1%, 1 L of water, pH 7.0±0.2, autoclaved at 121°C for 30 min.

[0042] Bacillus subtilis and Clostridium butyricum were respectively activated, and 10% of the inoculum amount was inserted into the respective culture medium, shaken at 200 rpm at 30°C for 2 to 3 days, and the culture was stopped after 80% of the bacteria formed spores. Bacillus subtilis fermentation level ≥3.0×10 9 cfu / g, Clostridium butyricum fermentation level ≥2.0×10 9 cfu / g. The powders were obtained by spray drying ...

experiment example 1

[0047] Experimental example 1: Application on piglets

[0048] Adopt the microbial feed additive of embodiment 2. Add Bacillus subtilis, Clostridium butyricum, Saccharomyces cerevisiae and microbial feed additives to piglets’ full-price compound feed at a ratio of 1:1000, stir well and wait for use. The total number of bacteria in the compound feed is ≥5.0×10 6 cfu / g, the total number of bacteria in Bacillus subtilis and Clostridium butyricum ≥2.0×10 6 cfu / g, the number of Saccharomyces cerevisiae ≥5.0×10 5 cfu / g.

[0049] In the experiment, 100 large white piglets that were healthy and weaned at the age of 22 days were randomly divided into 5 groups, 20 piglets in each group. Set up 4 experimental groups, respectively fed with feeds supplemented with Bacillus subtilis, Clostridium butyricum, Saccharomyces cerevisiae and compound microorganisms; set up 1 control group, fed with full-price compound feed, and did not carry out prevention and control methods such as antib...

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Abstract

The invention relates to a microbial feed additive and belongs to the field of an agricultural microbial feed additive. The microbial feed additive is prepared by compounding three microbes, namely bacillus subtilis, clostridium butyricum and saccharomyces cerevisiae according to a certain ratio, wherein bacillus subtilis and clostridium butyricum adopt liquid fermentation and saccharomyces cerevisiae adopts liquid-solid fermentation. As for solid culture, solid fermentation of a culture medium prepared by using lucid ganoderma cultural residue, wheat bran and the like as raw materials is carried out. The microbial feed additive is used for livestock breeding, can effectively raise immunologic functions such as disease resistance, disease prevention and the like of piglets and chicks and reduce death rate, and can be used in laying hens to enhance egg yield and improve egg quality. The microbial feed additive is of great significance for promoting sustainable development of poultry breeding industry and guaranteeing product quality and safety.

Description

technical field [0001] The invention relates to a microbial feed additive for livestock and poultry breeding, belonging to the field of agricultural microbial feed additives. Background technique [0002] In recent years, with the improvement of the intensification of the breeding industry, the spread and invasion of pathogens have gradually increased; in order to reduce the incidence and save breeding costs, farmers usually use antibiotics for prevention and control. Long-term abuse of antibiotics has led to low animal resistance and an increase in susceptible and resistant strains, making disease control and treatment more difficult, and antibiotics cannot overcome a series of problems such as intestinal microecological disorders, drug residues, and nutritional stress. Make livestock and poultry products, especially foods such as meat, eggs, milk, have great potential safety hazards, and cause great harm to people's health. [0003] Microbial feed additives are non-toxic,...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/16
Inventor 程辉彩张根伟张丽萍习彦花张飞燕崔冠慧尹淑丽董玉兰段普凡李书生
Owner BIOLOGY INST OF HEBEI ACAD OF SCI
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