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Rumen-protected lysine feed

A rumen-passing and lysine technology, applied in the field of amino acids, can solve problems such as increasing the workload of animal breeding and affecting the efficiency of animal absorption and utilization

Active Publication Date: 2015-03-25
NINGXIA EPPEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, when lysine is used as feed, because it is a small molecule nutrient, it can be released into the animal body soon, especially when it is ingested in large quantities, which affects the efficiency of animal absorption and utilization
Usually the solution is to feed the animals small and frequent, but this will increase the workload of animal husbandry

Method used

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  • Rumen-protected lysine feed

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Experimental program
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Effect test

preparation example Construction

[0029] In the second (1) aspect of the present invention, the present invention provides a feed additive containing L-lysine, which comprises coated core particles and a coating layer coated on the coated core particles, wherein the coating layer is Rumen fat, the preparation method of coated core particle comprises:

[0030] (1) Mix the lysine salt and filler and optional sodium glutamate evenly to form a mixture;

[0031] (2) adding an adhesive solution optionally containing xylanase to the mixture obtained in step (1), and mixing evenly to form a soft material;

[0032] (3) sieving the soft material obtained in step (2) to form coarse particles;

[0033] (4) Shot blasting the coarse particles obtained in step (3) to form round pellets; and

[0034] (5) Drying the round pellets obtained in step (4) and sieving to form coated core particles.

[0035] Preferably, the filler in step (1) is microcrystalline cellulose and / or dextrin.

[0036] Preferably, the binder in step (2...

Embodiment 1

[0150] The preparation of the expression construct of the xylanase gene of embodiment 1 mutant

[0151] According to the xylanase gene recorded in our former Chinese Patent No. 201210185883, the Arg encoding the 45th position on it is mutated into Ser, that is, the CGC at the 133-135th position of the nucleotide sequence is mutated into TCC, the mutant type The nucleotide sequence of the xylanase is shown in SEQ ID No: 1 of the sequence listing, and the amino acid sequence of the encoded xylanase is shown in SEQ ID No: 2. Then, according to the "Molecular Cloning Experiment Guide" and the operating guide of the commercial reagents used to construct the secretory expressed yeast, in short, the gene encoding the xylanase is used as a forward primer such as SEQ ID No: 3 of the sequence listing As shown (the EcoR I endonuclease site was introduced), the reverse primer was shown in SEQ ID No: 4 of the sequence table (the Not I endonuclease site was introduced) and EcoR I and Not I...

Embodiment 2

[0152] Embodiment 2 Fermentation preparation and activity determination of mutant xylanase

[0153] Inoculate the positive yeast transformant obtained in Example 1 into 50ml BMGY liquid medium (100mM potassium phosphate buffer (pH6.0), containing 1% yeast extract, 2% peptone, 1.34% yeast nitrogenous base, biological Prime 4*10 -5 %, glucose 2%, glycerol 1%), cultured at 30°C, 200rpm until OD600 reached 5.0.

[0154] Transfer the above culture to 1L BMGY liquid medium, cultivate at 30°C, 200rpm for 24 hours, add 5ml of methanol, and then continue to cultivate at 30°C, 200rpm for 8 days, during which 5ml of methanol was added every 24 hours, and the solution was controlled by ventilation. Oxygen (DO) is greater than 20%, and the pH is controlled at 6.0 (adjusted with ammonia water). After the cultivation is completed, filter with a 0.22 μm filter membrane and retain the supernatant, which is detected by SDS-PAGE to be rich in proteins corresponding to xylanase, thereby obtaini...

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Abstract

The invention provides a feed additive containing L-lysine. The feed additive consists of coated core particles and coating layers covering the coated core particles, wherein the coating layers are rumen-protected fat; and a preparation method of the coated core particles comprises the following steps: uniformly mixing lysine salt and a filler as well as optional sodium glutamate, adding an optional adhesive solution containing xylanase, sieving, shot-blasting, drying and sieving. In addition, the invention further provides a preparation method of the feed additive, wherein the preparation method comprises a step of spraying the rumen-protected fat on the coated core particles.

Description

technical field [0001] The invention belongs to the field of amino acids, in particular, the invention relates to a feed additive containing L-lysine and a preparation method thereof. Background technique [0002] L-lysine is an important amino acid raw material, which can be used as condiment, food, feed additive, and also as an effective or auxiliary ingredient in health care products and medicines. It is widely used in food industry, feed industry, pharmaceutical industry and other chemical industries. in industry. At present, the production of L-lysine is mainly produced by fermentation of microorganisms, such as coryneform bacteria. [0003] However, when lysine is used as feed, because it is a small molecular nutrient, it can be released into the animal body soon, especially when it is ingested in large quantities, which affects the efficiency of animal absorption and utilization. The usual solution is to feed the animals a small number of times, but this will incre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K1/165
Inventor 孟刚马吉银陈崇安
Owner NINGXIA EPPEN BIOTECH
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