Tussah silk protein adriamycin sustained release microsphere and preparation method thereof

Abstract

The invention discloses a tussah silk protein adriamycin sustained release microsphere and a preparation method thereof. The tussah silk protein adriamycin sustained release microsphere takes calcium ions as an inducer, natural tussah silk proteins as a shell and adriamycin as nano particles of a core, is uniform in distribution of particle sizes, has the diameter of about 500 nanometers and is biodegradable. According to the tussah silk protein adriamycin sustained release microsphere and the preparation method disclosed by the invention, as the preparation environment of the tussah silk protein adriamycin sustained release microsphere is in a neutral and normal pressure and temperature state, an organic solvent is avoided, and the utilization degree of a medicament is increased; with the tussah silk proteins as an embedding material of the adriamycin, the tussah silk protein adriamycin sustained release microsphere has an RGD sequence and is capable of promoting cells to identify the tussah silk protein adriamycin sustained release microsphere and improving the targeting property of an adriamycin medicament; compared with free adriamycin, the adriamycin embedded in the tussah silk protein microsphere is obvious in sustained release property, expresses pH sensitivity, is obvious in sustained release effect in the environment with a pH value of 5.2, is small in releasing speed under the neutral condition, is convenient for a medicament to be released after entering cancer cells and is capable of remarkably lowering the toxic and side effects in normal cells.

Description

technical field

[0001] The invention relates to a drug-loaded microsphere and a preparation method thereof, in particular to a microsphere prepared from tussah silk fibroin-doxorubicin hydrochloride as a raw material and a preparation method thereof, belonging to the technical field of biomedicine. Background technique

[0002] Malignant tumor is a common and frequently-occurring disease that seriously threatens human health. At present, there is no effective prevention and control measure. The main method of clinical treatment of cancer is radiotherapy and chemotherapy, but the antitumor drugs used in chemotherapy have serious toxic and side effects on normal organs of the human body. The targeted drug delivery system delivers the drug directly to the lesion site through the carrier material to exert its curative effect. It has the advantages of strong specificity, low toxicity and side effects, improved drug efficacy, reduced drug dose and frequency of administration, etc...

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