Tussah silk protein adriamycin sustained release microsphere and preparation method thereof
A technology of tussah silk fibroin and slow-release microspheres, which is applied in the field of biomedicine, can solve the problems of large diameter of microspheres, influence on drug activity and albumin activity, low drug loading rate and embedding rate, and reduce toxic and side effects , the effect of improving efficiency
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[0023] Example 1:
[0024] Put 80g of tussah silk in the environment of 98~100℃, put it in the environment containing 5g / L Na 2 CO 3 And 0.0625g / L sodium dodecyl sulfate solution for degumming 3 times, each 30min, the liquor ratio is 1:50. After degumming, tussah silk fibroin was obtained, which was dried at 60°C. The tussah silk fibroin fiber is placed in a saturated lithium thiocyanate solution at a liquor ratio of 1:10, dissolved at 50 ° C ± 2 ° C for 60 min, and the obtained tussah silk fibroin protein solution is loaded into a solution with a molecular weight cut-off of 8 to 10 KDa. In a dialysis bag, dialyzed with deionized water for 4 d, and then centrifuged to obtain a regenerated tussah silk fibroin solution with a mass concentration of about 30 mg / ml, which was placed in a 4°C refrigerator for use.
[0025] Adjust the concentration of tussah silk fibroin protein to 10 mg / ml, configure doxorubicin hydrochloride solution, the concentration is 10 mg / ml; configure c...
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[0032] Example 2
[0033] Put 120g tussah silk in a stainless steel pot of 4L deionized water, heat it to boiling with an induction cooker, add 0.06% Na 2 CO 3 In the aqueous solution, degumming 3 times, 35min each time, the liquor ratio is 1:60. After degumming, tussah silk fibroin was obtained, which was dried at 60°C. The tussah silk fibroin fiber was placed in a saturated lithium thiocyanate solution at a liquor ratio of 1:10, dissolved at 50 °C ± 2 °C for 70 min, and the obtained tussah silk fibroin solution was loaded into a dialysis with a molecular weight cut-off of 8-10 KDa. In the bag, dialyzed with deionized water for 4 d, and then centrifuged to obtain a regenerated tussah silk fibroin solution with a mass concentration of about 25 mg / ml, which was placed in a 4°C refrigerator for storage for later use.
[0034] Adjust the concentration of tussah silk fibroin to 20 mg / ml, configure doxorubicin hydrochloride solution with a concentration of 10 mg / ml; configure c...
Example Embodiment
[0035] Example 3
[0036] Put 180 grams of tussah silk into 8 L of sodium carbonate aqueous solution with a mass concentration of 0.05%, degumming at 98-100° C. for 0.5 hours, repeat the treatment for 3 times, and obtain pure tussah silk fibroin after thorough washing. The dried tussah silk fibroin fiber was heated and dissolved with 1 liter of lithium thiocyanate solution with a concentration of 9.3 mol / liter at 55±5° C. to obtain a mixed solution of tussah silk fibroin protein. Using a cellulose membrane as a dialysis material, the obtained tussah silk fibroin mixed solution was dialyzed with deionized water for 3 days to obtain a pure tussah silk fibroin solution.
[0037] Adjust the concentration of tussah silk fibroin protein to 30 mg / ml, configure doxorubicin hydrochloride solution, the concentration is 10 mg / ml; configure calcium gluconate solution, the concentration is 100 mmol per liter; add doxorubicin hydrochloride solution to tussah silk fibroin solution Mechanica...
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