Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bacterial strain capable of producing chitin deacetylase and application of bacterial strain in production of chitin deacetylase through fermentation

The invention relates to a technology for vegetative deacetylase strain and crude vegetal deacetylase enzyme, which is applied in the field of fermentation to produce chitin deacetylase, can solve the problems of affecting application, enzyme activity, low yield and the like, and achieves the effect of improving yield and low cost.

Inactive Publication Date: 2015-04-08
SOUTH CHINA UNIV OF TECH
View PDF2 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chitin deacetylase produced by most strains is an intracellular enzyme, and the enzyme activity and yield are low, which will affect the application of the enzyme in the chitin biotransformation process

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacterial strain capable of producing chitin deacetylase and application of bacterial strain in production of chitin deacetylase through fermentation
  • Bacterial strain capable of producing chitin deacetylase and application of bacterial strain in production of chitin deacetylase through fermentation
  • Bacterial strain capable of producing chitin deacetylase and application of bacterial strain in production of chitin deacetylase through fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Screening of enzyme-producing strain Aspergillus versicolor (Aspergillus versicolor) X

[0038]Primary screening of enzyme-producing strains: Weigh 10g of soil collected from chitin production waste, add 90mL of sterile water containing glass beads, shake at 200rpm for 30min, and after standing for 15min, take 0.5ml of the supernatant and add it to 4.5ml of sterile water, followed by serially diluted to 10 -7 , take 200 μL respectively, and spread on the solid screening medium. The solid screening medium was incubated at a constant temperature of 30°C for 4 days, and a single colony that turned yellow around the colony in the medium was picked, and the method of streak separation was used to continue purification, and repeated 3 times until the characteristics of the colonies were consistent. 27 strains producing chitin deacetylase were screened out, including 1 strain of yeast, 6 strains of bacteria and 20 strains of mold. Inoculate the completely purified single col...

Embodiment 2

[0041] Application of Aspergillus versicolor (Aspergillus versicolor) X chitin deacetylase production medium carbon source optimization experiment. Glucose, sucrose, xylose, lactose, maltose and soluble starch were respectively selected as the carbon source of the medium, the concentration was 8g / L, and the other components were 5g / L of bactochemical peptone, 1g / L of dipotassium hydrogen phosphate, and 0.5 g of magnesium sulfate. g / L, the initial pH of the medium was 6.0. The spore suspension of the strain was inoculated into the fermentation medium at an inoculation ratio of 6%, cultured at a constant temperature of 30° C. and 160 rpm for 96 hours, and the mycelium was separated by filtration, and the supernatant was the extracellular enzyme. The mycelium was crushed with a mortar, dissolved in 50mM PBS buffer, centrifuged at 12000rpm at 4°C, and the supernatant was taken as intracellular enzymes, and the enzyme activities of intracellular enzymes and extracellular enzymes we...

Embodiment 3

[0046] Nitrogen source optimization experiment using Aspergillus versicolor (Aspergillus versicolor) X chitin deacetylase production medium. Yeast extract, tryptone, soybean peptone, bacteriological peptone, urea, ammonium nitrate, ammonium chloride and ammonium sulfate were respectively selected as the nitrogen source of the medium, the nitrogen content was 1g / L, and the other components were glucose 10g / L. Dipotassium hydrogen phosphate 1g / L, magnesium sulfate 0.5g / L, and the initial pH of the medium was 6.0. The spore suspension of the strain was inoculated into the fermentation medium at an inoculation ratio of 6%, cultured at a constant temperature of 30° C. and 160 rpm for 96 hours, and the mycelium was separated by filtration, and the supernatant was the extracellular enzyme. The mycelium was crushed with a mortar, dissolved in 50mM PBS buffer, centrifuged at 12000rpm at 4°C, and the supernatant was taken as intracellular enzymes, and the enzyme activities of intracellu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a bacterial strain capable of producing chitin deacetylase and an application of bacterial strain in production of chitin deacetylase through fermentation. The bacterial strain is Aspergillus versicolor X, and is preserved at a China Center for Type Culture Collection, the preservation number is CCTCC NO.M2014459, and preservation date is 8th, Oct, 2014. The invention also provides a enzyme production method through fermentation, the enzyme activity of the prepared chitin deacetylase can reach 1.41U / mL. The application has the advantages that scope of enzyme microbe capable of producing chitin deacetylase is developed, production cost is reduced, and chitin deacetylase has wide application prospect.

Description

technical field [0001] The invention discloses a strain producing chitin deacetylase and a method for producing chitin deacetylase by fermenting the strain, belonging to the field of biotechnology. Background technique [0002] Chitin is the second largest biological polysaccharide next to cellulose on the earth, and it is also the only natural alkaline polysaccharide that exists in large quantities on the earth. It is widely found in the shells of insects, crustaceans, shrimps and crabs, and fungi ( Yeast, mold) and plant cell walls. The annual biosynthesis of chitin is about 10 billion tons. [0003] Chitin is a linear polymer connected by N-acetylamino-D-glucose through β-(1,4) glycosidic bonds. There are a large number of hydrogen bonds in the molecule, and its solubility is very poor. Chitosan is the product of partial or complete removal of the acetylamino group in chitin molecules. Due to the presence of a large number of free amino groups in its molecule, the molec...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/14C12N9/80C12R1/66
CPCC12N1/14C12N9/80C12Y305/01041C12N1/145C12R2001/66
Inventor 娄文勇程建华宗敏华徐培
Owner SOUTH CHINA UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com