Collection, storage, in-vitro culture and viability detection technology for acacia auriculiformis pollen

A technology of pollen collection and in vitro culture, which is applied to the determination/inspection of microorganisms, plant cells, and botanical equipment and methods, etc. and other problems, to achieve the effects of low natural pod setting rate, rapid operability, and uniform pollen distribution.

Inactive Publication Date: 2015-04-08
RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]Acacia (Acacia) species generally have a low seed setting rate, and the large-leaf Acacia (Acacia auriculiformis A. Cunn. ex Benth .) is no exception, this is one of the key issues restricting the further development of its planting industry
Acacia big-leaf fruit is low, and the flowering period is easily affected by bad weather such as rain and typhoon, resulting in low and unstable yields, which limits the development of Acacia big-leaf

Method used

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  • Collection, storage, in-vitro culture and viability detection technology for acacia auriculiformis pollen
  • Collection, storage, in-vitro culture and viability detection technology for acacia auriculiformis pollen
  • Collection, storage, in-vitro culture and viability detection technology for acacia auriculiformis pollen

Examples

Experimental program
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Effect test

Embodiment 1

[0032] In early August, when Acacia bigleaf blooms, collect fresh inflorescences before 8:00 and put them in sulfuric acid paper bags, lay them flat and dry them in the shade in a constant temperature room at 28°C, and wait until the anthers are completely burst. After that, brush the powder and sieve it for later use, add 1-2 drops of a suitable liquid medium (200g / L sucrose + 300 mg / L boric acid) on the groove of the glass slide, and dust the collected pollen on the liquid medium Put the glass slides into a petri dish for culture, put two pieces of wet filter paper in the petri dish, and keep the culture temperature at 30°C. After culturing for 3, 6, 9, 12, and 24 days, the number of pollen germination, the length of pollen tubes and the number of pollen tubes were observed with an optical microscope. For the Acacia macrophylla pollen collected on the same day, after 24 hours of cultivation, it can be observed that the pollen germination rate is 94%, the length of the pollen...

Embodiment 2

[0034] During the in vitro germination of Acacia macrophylla pollen, add 1-2 drops of a suitable liquid medium (200g / L sucrose + 300 mg / L boric acid) to the groove of the glass slide, and collect the pollen and store it at room temperature, The pollen stored at 5°C and -20°C for 1 month was put on the liquid culture medium, and the glass slides were placed in a petri dish for culture, and two pieces of wet filter paper were placed in the petri dish, and the culture temperature was kept at 30°C. After culturing for 3, 6, 9, 12, and 24 days, the number of pollen germination, the length of pollen tubes and the number of pollen tubes were observed with an optical microscope. The pollen of Acacia macrophylla preserved for 1 month, the pollen germination rate after storage at room temperature, storage at 5°C, and storage at -20°C for 24 hours was 28%, 42%, and 63%, respectively, and the length of the pollen tube could reach the longest pollen Three times the diameter, a single polle...

Embodiment 3

[0036] During the in vitro germination of Acacia macrophylla pollen, add 1-2 drops of a suitable liquid medium (200g / L sucrose + 300 mg / L boric acid) to the groove of the glass slide, and collect the pollen and store it at room temperature, The pollen stored at 5°C and -20°C for 1 week, 1 month, and 1 year were put on the liquid culture medium, and the glass slides were placed in a petri dish for culture, and two pieces of wet filter paper were placed in the petri dish. Keep the culture temperature at 30°C. After culturing for 3, 6, 9, 12, and 24 days, the number of pollen germination, the length of pollen tubes and the number of pollen tubes were observed with an optical microscope. The pollen germination rate of Acacia macrophyllum preserved for 1 year after storage at room temperature, storage at 5°C, and storage at -20°C for 24 hours was 13%, 28%, and 48%, respectively, and the length of the pollen tube was the longest. Twice the diameter, a grain of pollen can germinate ...

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Abstract

The invention discloses a collection, storage, in-vitro culture and viability detection technology for acacia auriculiformis pollen. The technology comprises the following steps: collecting acacia auriculiformis inflorescence blooming in the morning, placing into a sulfuric acid paper bag, and drying in the shade inside at 25-30 DEG C; collecting pollen when anthers greatly crack after 10 o'clock in the next morning, and screening by virtue of a sieve; putting the collected pollen into a small glass bottle, adding dry allochroic silicagel, vacuumizing and sealing with wax; storing the sealed pollen at 25 DEG C to -25 DEG C; and germinating the pollen, namely putting the stored pollen into a liquid culture medium and germinating at 25-35 DEG C. According to the method disclosed by the invention, the technical problems of collection, storage and germination of the acacia auriculiformis pollen are solved; the germination rate of the acacia auriculiformis pollen can be significantly improved; the germination of the acacia auriculiformis pollen stored at -20 DEG C for a year is still 48%; and compared with similar artificial hybridization pollination and artificial pollination of seed orchards, the technology disclosed by the invention has great significance on improvement of the seed yield and quality.

Description

technical field [0001] The invention belongs to the field of plant hybrid breeding, and in particular relates to a method for improving the pollen germination rate of Acacia macrophylla. Background technique [0002] Daye Acacia ( Acacia auriculiformis ) is a plant of the genus Acacia Mill. in the family Mimosaceae. Naturally distributed at 5°-17° south latitude, it is native to Queensland, Australia, southern and western Papua New Guinea, Irian Island, and Ki Island, Indonesia. Acacia big leaf has a large biomass and excellent material, which can be used as pulp material, building material, furniture material, etc.; it has nitrogen fixation effect, and can be used for improving soil and greening barren hills. However, Acacia tree species generally have a low seed setting rate, and Acacia macrophylla is no exception, which is one of the key problems restricting the further development of its planting industry. Acacia big-leaf fruit is low, and the flowering period is easi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04A01N3/00C12Q1/02
Inventor 詹妮黄烈健
Owner RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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