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Hericium erinaceus isolate and isolating method and application thereof

A technology of Hericium erinaceus culture and medicine, applied in the field of Hericium erinaceus isolate and its separation, can solve the problem of incomplete elucidation of the material basis of Helicobacter pylori, and achieve the effect of simple separation process and controllable process quality

Inactive Publication Date: 2015-04-22
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The current research has basically established the relationship between Hericium-chronic gastritis and peptic ulcer-Helicobacter pylori, but the material basis of Hericium's ability to inhibit Helicobacter pylori is still incomplete

Method used

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  • Hericium erinaceus isolate and isolating method and application thereof
  • Hericium erinaceus isolate and isolating method and application thereof
  • Hericium erinaceus isolate and isolating method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The separation method of Hericium erinaceus isolate is:

[0028] Take Hericium erinaceus solid fermentation mycelia crude extract dry powder 5kg (Hericium solid fermentation mycelia crude extract is that Hericium solid fermentation culture is extracted with hot water, concentrates, oven dry makes), adds 50L Distill the boiling water and stir well, let stand at room temperature after cooling, remove the supernatant, add 5L of pretreated macroporous resin (model: HPD-100) to the solution for adsorption for 24 hours, stir from time to time during this period, remove the supernatant, and obtain adsorption The macroporous resin after adsorption; put the macroporous resin after adsorption into a column (diameter-to-height ratio 1:8), and after elution with distilled water of 3 times the volume of the column bed, use the ethanol solution with mass concentration of 50% and 95% successively Carry out gradient desorption with 4 times the volume of the column bed, the flow rate is...

Embodiment 2

[0044] Bacteriostasis test:

[0045] 1), HP cultivation:

[0046] Melt the suspected solid improved Brooke’s broth medium in a microwave oven, wait until the temperature is cooled to about 50°C, add 15ml of fresh aseptic sheep blood under aseptic conditions, shake well, and quickly pour it into a sterilized plate. Plate about 20ml, wait for the blood plate to cool and solidify. Take Helicobacter pylori preserved in skimmed milk at -80°C, use a sterile inoculation loop to streak and gently inoculate on the surface of the blood plate, and put it in a three-gas incubator (O 2 ,5%; CO 2, 10%;N 2 , 85%), incubated at 37°C for 72 hours.

[0047] 2), bacterial suspension preparation:

[0048] Pick an appropriate amount of colonies cultured for 72 hours (HP) in sterile normal saline (0.9% NaCl), sonicate for about 30 s, and make an equivalent to 2.0 McFarland standard (containing bacteria 1×10 7 to 1×10 8 c.f.u.ml / L)

[0049] 3) MIC detection:

[0050] Use a 96-well plate (96...

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Abstract

The invention belongs to the field of biological medicine, and particularly relates to a hericium erinaceus isolate and an isolating method and application thereof. The isolating method comprises the steps that macroporous resin adsorption, elution and concentration are conducted on hericium erinaceus solid fermentative hypha crude extract, then diethyl ether is added for extraction, 200-300-mesh column chromatography on silica gel and gradient elution are conducted, and then a compound I is obtained through recrystallization. The compound I and the hericium erinaceus isolate can be used for preparing drugs for treating diseases caused by helicobacter pylori, especially diseases of digestive tract including gastritis, peptic ulcers, dyspepsia, ulcerative colitis and malignant tumors of digestive tract. The hericium erinaceus isolate can also serve as a food additive.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to an isolate of Hericium erinaceus and its isolation method and application. Background technique [0002] Since Marshall and Warren isolated Helicobacter pylori (Hp) from patients with gastritis in 1982, it has been confirmed that Helicobacter pylori is the pathogenic bacteria of chronic active gastritis and an important pathogenic factor of peptic ulcer. Persistent infection can lead to chronic atrophic gastritis. Compared with uninfected people, the risk of gastric cancer in infected people is 2-6 times higher (CDC, 1998). Helicobacter pylori eradication can significantly reduce the occurrence of gastric cancer. In addition, Helicobacter pylori may be closely related to many extragastric diseases (Chen Ying, 2009). Clinically, it is difficult to eradicate H. pylori infection with a single antibiotic, so different drug combinations are used, such as bismuth-based dual and ...

Claims

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Application Information

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IPC IPC(8): C07D493/08A61K36/06A61K31/352A61P31/04A61P1/04A61P1/14A61P35/00A23L1/30A23L1/28A23L31/00A23L33/105
CPCC07D493/08A23L31/00A23L33/10
Inventor 尚晓冬宋春艳章炉军于海龙张美彦徐珍王瑞娟杨慧张丹刘建雨谭琦刘瑞娜
Owner SHANGHAI ACAD OF AGRI SCI
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