Method for detecting phenolic acid and/or tanshinone ingredient by capillary electrophoresis

A technology of capillary electrophoresis and tanshinone, which is applied in the field of analysis and detection, can solve the problems of single stationary phase, large consumption of mobile phase, and high cost of the detection method, and achieve the effect of remarkable experimental detection effect, good stability, and simple steps

Active Publication Date: 2015-04-29
HANGZHOU NORMAL UNIVERSITY
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  • Abstract
  • Description
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Problems solved by technology

At present, there are many literature reports on the detection of various phenolic acids and tanshinone components in Salvia miltiorrhiza, but the detection method and the stationary phase used are relatively simple, mainly using liquid chromatography as the main detection system, but its cost is relatively high , requires a variety of chromatographic columns, and the mobile phase consumes a lot of money and is mostly toxic

Method used

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  • Method for detecting phenolic acid and/or tanshinone ingredient by capillary electrophoresis
  • Method for detecting phenolic acid and/or tanshinone ingredient by capillary electrophoresis
  • Method for detecting phenolic acid and/or tanshinone ingredient by capillary electrophoresis

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preparation example Construction

[0047] The preparation method of the Danshen reference substance solution related to the present invention refers to the 2010 edition of the Pharmacopoeia. The specific steps are: respectively take vanillic acid, rosmarinic acid, danshensu sodium, salvianolic acid A, salvianolic acid C, caffeic acid, and salvianolic acid The reference substance of acid B, protocatechuic acid, dihydrotanshinone and tanshinone IIA is appropriate, accurately weighed, mixed and placed in a volumetric flask, and methanol is added to make each lml containing vanillic acid, rosmarinic acid, danshensu sodium, salvianol A solution of acid A, salvianolic acid C, caffeic acid, salvianolic acid B, protocatechuic acid, and 250 μg each of dihydrotanshinone and tanshinone IIA to obtain the reference solution.

[0048] Precisely measure 200μL of the reference solution into a 1.5mL centrifuge tube, add 100μL of blank urine extract and 200μL of blank buffer solution, vortex for 3 seconds, filter, and take the addit...

Embodiment 1

[0050] Example 1. Investigation on the concentration of graphitized multi-walled carbon nanotubes

[0051] 1.1 Take 4 clean 30ml wide-mouth bottles, numbered 1, 2, 3, and 4. Among them, add 6.5mL water to bottle 1; add 0.75mL40μg / mL graphitized multi-walled carbon nanotube dispersion and 5.75 to bottle 2 mL water; add 1.5mL40μg / mL graphitized multi-walled carbon nanotube dispersion and 5mL water to bottle 3; add 2.25mL40μg / mL graphitized multi-walled carbon nanotube dispersion and 4.25mL water to bottle 4, respectively Add 86.5 mg of SDS, 2.5 mL of 40 mmol / L borax and 1 mL of isopropanol to the four bottles, sonicate for 10 minutes, and prepare a buffer solution.

[0052] 1.2 Take 4 organic membrane filters of 0.22μm size, corresponding to No. 1-4 syringe numbers 1, 2, 3, 4, and filter the buffer solution in the syringe with the filter according to the corresponding number, a buffer solution Corresponding to two injection bottles, a total of 8 injection bottles are loaded.

[0053]...

Embodiment 2

[0058] Example 2. Investigation of SDS concentration

[0059] 2.1 Take 4 clean 30ml jars, numbered 1, 2, 3, 4, of which 57.5mg SDS is added to bottle 1; 86.5mg SDS is added to bottle 2; 115.4mg SDS is added to bottle 3; Add 144.2mg SDS to the bottle, and then add 1.5mL40μg / mL graphitized multi-walled carbon nanotube dispersion, 5mL water, 2.5mL40mmol / L borax and 1mL isopropanol to the four bottles respectively, and ultrasound for 10 minutes to prepare buffer Solution.

[0060] 2.2 Take four 0.22μm organic membrane filters, corresponding to the number 1-4 syringe number 1, 2, 3, 4, and according to the corresponding number, use the filter to filter the buffer solution in the syringe, a buffer solution Corresponding to two injection bottles, a total of 8 injection bottles are loaded.

[0061] Fill the capillary column with a buffer solution, and take a blank urine sample plus a reference substance for capillary electrophoresis detection.

[0062] The experimental results are as follow...

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Abstract

The invention relates to a method for detecting a phenolic acid and / or tanshinone ingredient by capillary electrophoresis. According to the method, the capillary electrophoresis separation of different phenolic acid and tanshinone ingredients in salvia miltiorrhiza herbs and biological fluid can be realized, a capillary column is filled with a buffer solution, the buffer solution comprises the following ingredients: graphitization multiwall carbon nanotube with final concentration of 3 to 9 microgram / mL, lauryl sodium sulfate B with concentration of 20 to 50 mmol / L, isopropanol with volume percentage of 7.5 to 15 percent and 5 to 20 mmol / L of sodium borate, the pH value is 7 to 9, the graphitization multiwall carbon nano tube is added in a form of carbon nano tube dispersion liquid, the capillary electrophoresis detection is carried out on a sample, and the content of the phenolic acid and tanshinone ingredient in the sample can be calculated according to a standard curve of a reference product. According to the method, the graphitization multiwall carbon nano tube with functionalized surface active agent is firstly used as a novel fixed phase into the solution to form the buffer solution with good stability and uniformity in dispersion, a good effect for separating the phenolic acid from tanshinone ingredients can be realized, and a remarkable effect on the detection aspect of the salvia miltiorrhiza herbs and biological fluid can be achieved.

Description

Technical field [0001] The invention relates to a novel stationary phase of capillary electrophoresis, which can realize the electrophoresis separation of different phenolic acids and tanshinone components in salvia medicinal materials and biological fluids, and belongs to the technical field of analysis and detection. Background technique [0002] Capillary electrophoresis (CE) is a new type of liquid phase separation technology that uses a high-voltage direct current electric field as a driving force and a capillary as a separation channel, according to the difference in distribution capacity and electrophoretic mobility between sample components. Its application It has been involved in many fields such as environmental testing, medical testing and life sciences. There are many kinds of stationary phases in capillary electrokinetic chromatography, mainly micelles formed with surfactants and microemulsion buffer solutions. In recent years, the application of nanomaterials, espe...

Claims

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Application Information

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IPC IPC(8): G01N27/447
Inventor 曹君曹婉刘训高胡帅帅庞潇卿
Owner HANGZHOU NORMAL UNIVERSITY
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