Preparation of a multi-walled carbon nanotube composite material modified by folic acid and chitosan

A technology of multi-walled carbon nanotubes and chitosan, which can be used in medical preparations, drug combinations, and pharmaceutical formulations with non-active ingredients, can solve problems such as solubility and biocompatibility limitations, and achieve good biodegradability. and biocompatibility, uniform appearance, no toxic and side effects

Inactive Publication Date: 2015-05-27
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, its solubility and biocompatibility have always limited its application in the field of biomedicine, so the modification of carbon nanotubes has become the key

Method used

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  • Preparation of a multi-walled carbon nanotube composite material modified by folic acid and chitosan
  • Preparation of a multi-walled carbon nanotube composite material modified by folic acid and chitosan
  • Preparation of a multi-walled carbon nanotube composite material modified by folic acid and chitosan

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Example 1: Acidification of multi-walled carbon nanotubes.

[0030] Add multi-walled carbon nanotubes (100 mg) and a certain amount of nitric acid (50 ml) into a 100 ml three-necked bottle, and ultrasonicate for 30 min. After the ultrasonication is completed, reflux at 120° C. for 10 h, stop the reaction, and stand overnight. After separating the nitric acid in the upper layer, dilute with distilled water and let stand again. Repeat the above operation, separate the upper aqueous solution, and then add distilled water to the remaining reaction solution for dilution. Centrifuge the reaction solution at 7000r / min for 3min, add water to dilute and centrifuge, remove the supernatant, repeat 2-3 times until the upper liquid is ink-colored. The supernatant was separated, and the obtained solid was dried overnight under an infrared lamp to obtain acidified carbon nanotubes.

Embodiment 2

[0031] Example 2: Characterization of acidified carbon nanotubes.

[0032] Infrared data of acidified carbon nanotubes show that 1640cm -1 The absorption peak produced by the stretching vibration of the carbon-oxygen double bond is at 3400cm -1 is the absorption peak produced by the oxygen-hydrogen stretching vibration. The above conclusions indicate that the nanotubes have been modified with acidic groups. (see figure 1 )

[0033] Measuring the zeta potential of multi-walled carbon nanotubes before and after acidification shows that the zeta potential value of carbon nanotubes before acidification is 32.4mv, and the potential of carbon nanotubes after acidification drops to -28.2mv, indicating that the surface of carbon nanotubes has been connected with negatively charged groups. That is, the carboxyl group has been successfully attached.

Embodiment 3

[0034] Embodiment 3: Preparation of folic acid activated ester.

[0035] Add folic acid (882mg) and anhydrous dimethyl sulfoxide (20ml) in a 50ml three-necked bottle, and add condensing agent dicyclohexylcarbodiimide (DCC), N-hydroxysuccinimide (NHS) and A certain amount of triethylamine was reacted overnight at 45°C in the dark under the protection of nitrogen. Stop the reaction, let the reaction solution stand still, remove the white by-product dicyclohexyl urea by suction filtration, drop the mother liquor into anhydrous ether solution containing 30% acetone, precipitate a yellow solid, filter, and dry the obtained solid in vacuum to obtain folic acid activated ester .

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Abstract

The invention relates to a multi-walled carbon nanotube modification method. Multi-walled carbon nanotubes (MWCNT) are acidified by a heating and refluxing manner, and have an inner diameter of about 6 nm through representation by a of Fourier infrared spectrum (FT-IR), a zate potentiometer, transmission electron microscopy (TEM), and other manners. In addition, a folic acid-chitosan (FA-CHI) conjugate is prepared by a homogeneous synthesis method and is reacted with the acidified nanotubes to form an FA-CHI-MWCNT composite nanometer material the inner diameter of which is about 26 nm. The synthesized composite nanometer material improves biocompatibility and targeting performance to a certain degree, and will have a good application prospect in the fields of biology and medicine. The surface modification method is short in process steps, less in used devices and materials and good in application prospect and is an ideal multi-walled carbon nanotube modification method.

Description

Technical field: [0001] The invention belongs to the field of nanometer material science and relates to a novel drug-carrying material for tumor targeting therapy. It specifically relates to the preparation of a new carrier with targeting and good biocompatibility—folic acid and chitosan-modified multi-walled carbon nanotube composite material. Background technique: [0002] Carbon nanotubes (CNTs) are hollow cylinders rolled into graphite sheets made of carbon atoms. Carbon nanotubes have a tubular structure, so they are more suitable as drug carriers in biological environments than spherical particles and flake particles. First of all, carbon nanotubes have good flexibility and can increase the contact area with cells through bending; secondly, they can smoothly enter cells through active endocytosis and passive diffusion; finally, the surface of carbon nanotubes and drugs There are strong π-π interactions and electrostatic interactions between molecules, which can effic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/48A61K47/36A61K47/22A61P35/00A61K47/69
Inventor 王越李祯李红玫何正大
Owner CHINA PHARM UNIV
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