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Fluorescent-CT bimodal imaging probe and preparation method thereof

A-CT, dual-mode technology, applied in fluorescence-CT dual-mode iodine-doped carbon quantum dots and its application in biomedical imaging, fluorescence-CT dual-mode imaging probes and their preparation fields, can solve the problem of Short residence time, lack of tissue-specific viscosity and osmotic pressure, etc., to avoid side effects, reduce the risk of adverse reactions, and strong X-ray attenuation

Inactive Publication Date: 2015-06-24
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this short residence time in vivo, lack of tissue specificity, and side effects caused by the inherent viscosity and osmotic pressure of iodide limit its wide clinical application.

Method used

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  • Fluorescent-CT bimodal imaging probe and preparation method thereof
  • Fluorescent-CT bimodal imaging probe and preparation method thereof
  • Fluorescent-CT bimodal imaging probe and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Dilute 0.1 g of glycine and 0.9 g of iodixanol to 20 mL of double-distilled water, and stir magnetically at room temperature (15-25°C) to fully dissolve to obtain a uniform and transparent solution. Add the above solution into a hydrothermal reaction kettle with polytetrafluoroethylene, heat at 180°C for 4 hours, and after the solution is naturally cooled, filter with medium-speed filter paper to remove insoluble black precipitates, centrifuge at 15,000 g for 30 minutes to remove large particles, and collect The supernatant was injected into a dialysis bag with a molecular cut-off of 2000 Da for dialysis. The dialysis time was 72 h, and the water was changed every 12 h. The dialyzed product was rotovaped to obtain a concentrated solution. The concentrated solution is freeze-dried at -50° C. to powder to obtain gadolinium-doped carbon quantum dots. Yield is 1.2%.

Embodiment 2

[0031] Dilute 0.3 g of glycine and 0.7 g of iodixanol to 20 mL of double-distilled water, and stir magnetically at room temperature (15-25°C) to fully dissolve them to obtain a uniform and transparent solution. Add the above solution into a hydrothermal reaction kettle with polytetrafluoroethylene, heat at 180°C for 4 hours, and after the solution is naturally cooled, filter with medium-speed filter paper to remove insoluble black precipitates, centrifuge at 15,000 g for 30 minutes to remove large particles, and collect The supernatant was injected into a dialysis bag with a molecular cut-off of 2000 Da for dialysis. The dialysis time was 72 h, and the water was changed every 12 h. The dialyzed product was rotovaped to obtain a concentrated solution. The concentrated solution is freeze-dried at -50° C. to powder to obtain gadolinium-doped carbon quantum dots. The yield was 2.6%.

Embodiment 3

[0033]Dilute 0.5 g of glycine and 0.5 g of iodixanol to 20 mL of double-distilled water, and stir magnetically at room temperature (15-25°C) to fully dissolve to obtain a uniform and transparent solution. Add the above solution into a hydrothermal reaction kettle with polytetrafluoroethylene, heat at 180°C for 3 hours, and after the solution is naturally cooled, filter with medium-speed filter paper to remove insoluble black precipitates, centrifuge at 15,000 g for 30 minutes to remove large particles, and collect The supernatant was injected into a dialysis bag with a molecular cut-off of 2000 Da for dialysis. The dialysis time was 72 h, and the water was changed every 12 h. The dialyzed product was rotovaped to obtain a concentrated solution. The concentrated solution is freeze-dried at -50° C. to powder to obtain gadolinium-doped carbon quantum dots. The yield was 4.3%.

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Abstract

The invention relates to a fluorescent-CT bimodal imaging probe and a preparation method thereof, specifically relates to a fluorescent-CT bimodal iodine doped carbon quantum dot prepared by a one-step hydrothermal carbonization method and application of the fluorescent-CT bimodal iodine doped carbon quantum dot in biomedical images, and belongs to the field of biomedical materials. The specific scheme is as follows: dissolving iodixanol and glycine in an aqueous solution according to a certain mass ratio to serve as precursors, putting the solution in a hydrothermal reaction kettle with polytetrafluoroethylene lining, heating up to 120-200 DEG C to react for 4 hours, dialyzing and freeze drying nigger-brown reaction liquid to remove residues and water to obtain black powdery nanoparticles, namely iodine doped carbon quantum dots. The iodine doped carbon quantum dot prepared by the method has such excellent properties as stable fluorescence, efficient X-ray attenuation capacity and good biocompatibility, and is successfully applied to live cell fluorescent imaging in vitro and CT imaging in vivo. The method has the advantages of simple operation, environmental protection and easy mass production.

Description

technical field [0001] The invention relates to a fluorescence-CT dual-mode imaging probe and a preparation method thereof, in particular to a fluorescence-CT dual-mode iodine-doped carbon quantum dot prepared by a one-step hydrothermal carbonization method and its application in biomedical imaging, belonging to field of biomedical materials. Background technique [0002] Computer Tomography (CT) is the most widely used clinical [0003] The non-invasive imaging examination method is currently the most mature and effective method for disease diagnosis and treatment and surgical guidance (Stewart, R.C., et al., Contrast-enhanced CT with a high-affinity cationic contrast agent for imaging ex vivo bovine, intact ex vivo rabbit, and in vivo rabbit cartilage. Radiology, 2013. 266(1): p. 141-50.). The basic principle of CT contrast-enhanced imaging is to collect and process images by computer according to the different attenuation capabilities of different tissues of the human ...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K49/04
Inventor 杜凤移居会祥孙明忠张苗苗张丽赵雪芬房正邹
Owner JIANGSU UNIV
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