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A kind of Bacillus amyloliquefaciens ba-ka3 and its application

A technology of amylolytic spores and bacillus, which is applied in the field of microbial technology and biological control, can solve the problems of easy killing of beneficial microorganisms, resistance of pathogenic bacteria, and increased cost of tomato gray mold prevention, so as to promote crop growth and broad-spectrum inhibition. The effect of bacterial activity

Active Publication Date: 2018-05-08
BIOLOGY INST OF HEBEI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, chemical pesticides are still the main measures to prevent and control the disease, but long-term use not only induces the resistance of pathogenic bacteria, but also increases the cost of tomato gray mold control and easily kills beneficial microorganisms in the environment

Method used

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  • A kind of Bacillus amyloliquefaciens ba-ka3 and its application
  • A kind of Bacillus amyloliquefaciens ba-ka3 and its application
  • A kind of Bacillus amyloliquefaciens ba-ka3 and its application

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Experimental program
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Effect test

Embodiment 1

[0023] The separation of embodiment 1 bacillus amyloliquefaciens BA-KA3

[0024] Bacillus amyloliquefaciens BA-KA3 was isolated from the rhizosphere soil of tomatoes in Liulintun Township, Luancheng County, Shijiazhuang City, Hebei Province.

[0025] The specific method is as follows: the rhizosphere soil of tomatoes was collected in the protected area of ​​Liulintun Township, Luancheng County, Shijiazhuang City, Hebei Province, and stored at 4°C for the isolation of antagonistic bacteria. Dilute 5g of tomato rhizosphere soil with 0.9% saline to 10 -4 、10 -5 、10 -6 . Pipette 100 μL each into the PB medium and spread evenly with a glass spreader. After culturing in an incubator at 28°C for 3 days, bacteria with different colony shapes were picked for isolation and purification. Using Botrytis cinerea as an indicator bacterium, the antagonistic bacteria of Botrytis cinerea were screened by plate confrontation method. After culturing in the dark at 22°C for 5 days, the strai...

Embodiment 2

[0027] The identification of embodiment 2 Bacillus amyloliquefaciens BA-KA3

[0028] The strain BA-KA3 does not produce pigment when grown on LB medium, and the colonies are flat or round, milky white and opaque, and the edges of the colonies are irregular. The surface is wrinkled. Gram-positive, rod-shaped, spore-forming, spore-elliptic or cylindrical. Refer to the "Common Bacteria Identification Manual" for physiological and biochemical identification of strains: V-P test positive, methyl red test negative, indole test positive, gelatin liquefaction test positive, citrate utilization test positive, starch hydrolysis test positive, lipase negative, h 2 S production test positive, nitrate reduction test positive, malonate utilization negative, tyrosine hydrolysis negative, cellulolysis positive.

[0029] 16S rDNA sequence analysis of bacterial strains: the molecular identification of strains is carried out by amplifying 16S rDNA of antagonistic bacteria.

[0030] Forward p...

Embodiment 3

[0036] Example 3 Indoor control effect test of Bacillus amyloliquefaciens BA-KA3 on tomato

[0037] Using sterile water as a negative control and azoxystrobin as a commonly used chemical control, the control effects of Bacillus amyloliquefaciens BA-KA3 sterile fermentation broth and bacterial suspension on tomato detached leaves, fruits and potted tomatoes were determined.

[0038] The tomato fruit and detached leaves were subjected to surface disinfection treatment, soaked in 75% alcohol for 30 seconds, and washed three times with sterile water. The cell concentration was 1×10 9 CFU / mL of Bacillus amyloliquefaciens BA-KA3, centrifuge at 4°C, 8000rpm for 20min, take the supernatant, remove the bacteria with a 0.22μm microporous membrane to obtain a sterile fermentation broth; resuspend the precipitate with sterile water to obtain a bacterial Suspension.

[0039]Determination of the control effect of BA-KA3 on tomato botrytis cinerea in vitro: sterilized pipette tip to punch ...

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Abstract

The present invention relates to a Bacillus amyloliquefaciense (Bacillus amyloliquefaciense) BA-KA3 and its application. The Bacillus amyloliquefaciense was preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on October 23, 2013, and the preservation number is : CGMCC No.8342; It has obvious antagonistic effect on Botrytis cinerea, wheat scab, wheat sheath blight, tobacco red spot, capsicum phytophthora, capsicum anthracnose, peanut brown spot, pear black Various pathogenic fungi such as spot bacterium and garlic sclerotinia have obvious inhibitory effect; and can significantly improve plant growth indicators such as seed germination rate, germination rate and root length; especially suitable for making agricultural preparations for preventing and treating tomato due to The disease caused by Botrytis cinerea infection and has a certain growth-promoting effect.

Description

technical field [0001] The present invention relates to a kind of Bacillus amyloliquefaciens ( Bacillus amyloliquefaciense ) BA-KA3 and its application belong to the field of microbial technology and biological control. Background technique [0002] Botrytis cinerea is a fungal disease of tomato caused by Botrytis cinerea, which can damage tomato stems, leaves, flowers and fruits. After the onset spreads rapidly, it poses a great threat to tomato production, especially in winter and spring seasons in the north. The low temperature and high humidity climate conditions in the shed provide a favorable environment for the infection and spread of Botrytis cinerea. Cause tomato production to reduce 20%~40%, can reach more than 60% when serious. At present, chemical pesticides are still the main measures to prevent and control the disease, but long-term use not only induces the resistance of pathogenic bacteria, but also increases the cost of the control of Botrytis cinerea and e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00A01P21/00C12R1/07
CPCA01N63/00A01N63/10C12N1/205C12R2001/07
Inventor 刘昆昂黄亚丽姜倍倍马宏贾振华郝楠张晶晶宋聪赵芊黄媛媛刘方张立文宋水山
Owner BIOLOGY INST OF HEBEI ACAD OF SCI
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