Recombinant halohydrin dehalogenase, encoding gene, vector, engineering bacteria, and applications of recombinant halohydrin dehalogenase

A technology of halohydrin dehalogenase and coding gene, which is applied in the field of recombinant halohydrin dehalogenase, can solve the problems of low catalytic efficiency and poor thermal stability tolerance, and achieve the effect of high catalytic activity and good industrial application prospects
CN104774828AActive Publication Date: 2015-07-15ZHEJIANG UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG UNIV OF TECH
Publication Date
2015-07-15

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Abstract

The present invention discloses a recombinant halohydrin dehalogenase, an encoding gene, a recombinant expression vector containing the gene, a recombinant bacterial containing the gene, and applications of the recombinant halohydrin dehalogenase in catalysis synthesis of (R)-4-cyano-ethyl 3-hydroxybutyrate through dehalogenation of (S)-4-chloro-ethyl 3-hydroxybutyrate. Compared with other halohydrin dehalogenases, the halohydrin dehalogenase obtained through the method has characteristics of high catalytic activity, high concentration substrate and product tolerance, and industrial application prospects.
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Description

(1) Technical field

[0001] The invention relates to a recombinant halohydrin dehalogenase, a coding gene, a carrier, a recombinant genetically engineered bacterium, and the key chiral intermediate (R)-4-cyano-3 for preparing atorvastatin by the recombinant halohydrin dehalogenase - Ethyl hydroxybutyrate application. (2) Background technology

[0002] Halohydrin dehalogenases, also known as halohydrin-hydrohalide lyases, catalyze the conversion of aromatic or aliphatic ortho-halohydrins to epoxides and hydrogen halides through an intramolecular nucleophilic substitution mechanism. Halohydrin dehalogenase can not only catalyze the cleavage of carbon-halogen bonds for dehalogenation reactions, but also catalyze and accept a series of unnatural nucleophiles other than halide ions with high selectivity, such as N 3 - , NO 2 - 、CN - etc. mediated epoxide ring-opening reactions. Halohydrin dehalogenase mainly forms a hydrogen bond between the conserved serine in the protein s...

Claims

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