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Oligopeptide molecules, and preparation methods and applications thereof

An oligopeptide and molecular weight technology, applied in the protein field, can solve the problems of high recurrence rate and patient pain, and achieve the effect of obvious effect, obvious anti-inflammatory and analgesic, and anti-tumor immunity.

Inactive Publication Date: 2015-08-05
陈光健
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Suffering to patients due to high recurrence rate

Method used

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  • Oligopeptide molecules, and preparation methods and applications thereof
  • Oligopeptide molecules, and preparation methods and applications thereof
  • Oligopeptide molecules, and preparation methods and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The preparation method of oligopeptide in embodiment 1 present invention

[0036] Raw material: Resin (Wang Resin), Fmoc protected amino acid,

[0037] Reagents: N,N-dimethylamide (DMF), DCM, MEOH, acetic anhydride, pyridine, DIEA, HBTU, hexahydropyridine

[0038] Instrument: 12-channel semi-automatic peptide synthesizer, high performance liquid chromatography (HPLC), model: Waters2695 Detection reagent: phenol reagent, pyridine, ninhydrin reagent

[0039] (1) The synthesis order is from C-terminal to N-terminal.

[0040] (2) Take 10mmol equivalent resin and put it into the polypeptide synthesizer reactor, add DCM (dichloromethane) to swell for half an hour, then remove the DCM, add 10mmol of the first Fmoc-protected amino acid in the sequence, 20mmol of DIEA (dichloromethane) Isopropylethylamine), an appropriate amount of DMF (dimethylformamide), DCM solution (appropriate amount means that the resin can be fully agitated), and react with nitrogen gas bubbling for 60 ...

Embodiment 2

[0048] Example 2 The effect of oligopeptide on the swelling degree of mouse auricle, the effect of anti-inflammation, detumescence and analgesia

[0049] Take Kunming mice, half male and half female, weighing 25-30 g, and randomly group them according to the sample size: 10 mice in each group. The doses of crude extracts of HY01-HY24 (250mg / kg) (2mg / ml), nabumetone (250mg / kg), and normal saline control group (0.1ml / 10g) were converted into the original animal crude drug doses (the same below). All groups were given oral administration, and the control group was given normal saline by oral administration. Each group was gavaged once a day for 4 consecutive days. 2 hours after the last gavage, Evan's blue 50mg / kg was injected into the tail vein, and 25ul of xylene was applied to both sides of the right ear of the mouse. Both ears were cut off from the baseline of the profile, and round ear pieces were punched on the same part of both ears with a puncher with a diameter of 7.5mm...

Embodiment 3

[0055] Embodiment 3 The influence of oligopeptide on the writhing response of mice caused by acetic acid, analgesic effect

[0056] Take Kunming mice, half male and half female, body weight 25-30g, randomly divided into groups, 14 oligopeptide administration group (250mg / kg), control group 5% aspirin soluble starch solution (250mg / kg), normal saline Group. 2 hours after the last gavage in the treatment group and the normal saline group, 0.6% glacial acetic acid was injected into the intraperitoneal cavity respectively, and the number of writhing caused by acetic acid in each group was observed within 15 minutes after giving 0.2ml of pain-inducing agent. The patients were treated as no writhing reaction.

[0057] Table 2

[0058]

[0059]

[0060] The results showed that the oligopeptide group could effectively reduce the abdominal pain response to acetic acid in mice, among which CD01-CD04 and CD07-CD12 had better effects.

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Abstract

Disclosed are oligopeptide molecules, preparation methods therefor and uses thereof. Said oligopeptides comprise CD1、CD2、CD3、CD4、CD5、CD6、CD7、CD8、CD9、CD10、CD11、CD12、CD13 and CD14. The amino acid sequences are shown in SEQ ID NO: 1-14 respectively. The oligopeptides can be prepared by synthesis, extraction or genetic engineering etc. Also disclosed are the uses of said oligopeptides in the preparation of anti-inflammatory and analgesic, wound healing and immunity enhancing drugs.

Description

technical field [0001] The invention belongs to the field of proteins, in particular to oligopeptides and applications thereof. Background technique [0002] Oligopeptides are a classification of polypeptides. The molecular weight segment is generally below 1000 Daltons, also known as small peptides, generally composed of 2-6 amino acids. [0003] Inflammation is the defense response of living tissue with vascular system to damaging factors. The response to the damaged local tissue of the body is called an inflammatory response. In the later stage of inflammation, exudate will be produced, and the oppression of the exudate and the action of inflammatory mediators can cause pain in patients. Now the anti-inflammatory clinical application drugs are mainly chemical synthetic drugs, such as nabumetone, indomethacin, piroxicam, ibuprofen, etc. Although these chemical drugs have obvious anti-inflammatory effects, they cannot cure the root cause and cannot eliminate the inflammato...

Claims

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Application Information

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IPC IPC(8): C07K5/087C07K5/097C07K5/078C07K5/065C07K5/062C07K5/072C07K5/093C07K1/06C07K1/04A61K38/06A61K38/05A61P29/00A61P17/02A61P1/04A61P37/04A61P31/00A61P35/00A23L1/29A23L33/00
CPCA23L33/18A23V2002/00A61K38/00A61P1/04A61P17/02A61P29/00A61P31/00A61P35/00A61P37/04C07K5/06043C07K5/06078C07K5/06104C07K5/0812C07K5/0819C07K5/0821A23V2200/318A23V2200/32A23V2250/55
Inventor 陈光健
Owner 陈光健
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