Long-time preservation method of magnaporthe oryzae strain

Abstract

The invention provides a long-time preservation method of a magnaporthe oryzae strain. Rice stalks are taken as the preservation carriers to preserve the magnaporthe oryzae; specifically, a culture medium is poured onto a culture dish flat plate; after the culture medium is solidified, the rice stalks are evenly placed on the culture medium immediately; after the rice stalks absorb the condensate of the culture medium and few nutrient substances carried in the condensate, the magnaporthe oryzae strain is inoculated to the center of the culture medium without rice stalks and enabled to grow and reproduce on the rice stalks; next, the rice stalks with the magnaporthe oryzae are dried at a constant temperature of 30 DEG C and then placed at a temperature within the range of -80 DEG C to 4 DEG C, and therefore, the long-time preservation of the magnaporthe oryzae strain is realized. The preservation method is simple, convenient and quick to operate; the treated magnaporthe oryzae strain is long in preservation period and stable in strain characters; besides, regular transplant is not required in the preservation period, and the preservation cost and the preservation space can be saved; the preservation method is safe and reliable, and also capable of activating the strain more conveniently; in short, the preservation method has important application value for study on the biological characteristics of the magnaporthe oryzae and the development of rice disease-resisting breeding.

Description

technical field [0001] The invention belongs to the technical field of microbiology and plant protection, and in particular relates to a method for long-term preservation of rice blast fungus strains. Background technique [0002] Magnaporthe oryzae Magnaporthe grisea Rice blast caused by Barr. is one of the main diseases of rice, and the annual rice loss caused by it can account for 10%-30% of the total rice output, which is one of the key factors hindering high and stable rice yield. According to the period of onset and the location where rice blast fungus infects rice, rice blast can be divided into seedling blast, leaf blast, panicle blast and grain blast, among which the panicle blast has the most serious impact on rice yield and quality. . Planting disease-resistant varieties is the most economical and effective measure to control rice blast, but a single disease-resistant rice variety will easily lose its disease resistance after 3-5 years of widespread planting, re...

AI Technical Summary

Problems solved by technology

Among them, the method of regular transplantation and preservation of strains is to inoculate the strains on a suitable solid slant medium, and after the strains are full of the slant, wrap the tampon with sterilized newspaper and store it in a refrigerator at 4°C. It is easy to operate, easy to use, can avoid the damage of mites, and can check whether the preserved strains are infected with miscellaneous bacteria at any time; but its disadvantage is that the growth and metabolism of the strains are not completely inhibited, the strains are easy to mutate, and the composition of the medium It will be gradually consumed by the strains, and needs to be transplanted every 6-8 months. At the same time, there are problems such as requiring a large storage space and glassware is easy to break.

The mineral oil preservation method is to add a layer of sterilized paraffin oil on the slope of the grown strain. The amount of paraffin oil exceeds the top of the slope of the strain by 1cm. It can be stored at room temperature or in the refrigerator. The species has a long survival period. Although the growth of the bacteria does not stop under mineral oil, it is obviously inhibited. The medium is not easy to dry, does not need to be transplanted frequently, and is not easy to be harmed by mites; its disadvantage is that it requires a large storage space and glassware is easy to At the same time, due to the mineral oil outside the mycelium, the growth of the strain is slow and the appearance is sticky when it is restored, which makes the restoration of the strain more cumbersome. Generally, it needs to be transplanted 1-2 times to get a good strain

The freeze-drying preservation method is to suspend the spores of the strain in the protective agent, and then divide a small amount of the spore suspension into sterilized ampoules under aseptic conditions, then connect the desiccator and the suction system, and put the ampoules in In the mixed freezing solution of dry ice and ethyl acetate, the suspension is completely dried by pumping air, and the ampoule is sealed and stored at room temperature or in the refrigerator. The advantage is that the storage time is long and the bacteria are not easy to mutate; No spores are produced, only mycelium type fungi are preserved, and special equipment is required, which is expensive

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