Long-time preservation method of magnaporthe oryzae strain

A technology for long-term preservation of Magnaporthe grisea, which is applied in the fields of microbiology and plant protection. It can solve the problems of difficult drying of the culture medium, cumbersome recovery of strains, and sticky appearance, so as to avoid the degradation of Magnaporthe grisea strains, Save storage space and avoid fragile and fragile effects

CN104877909AInactive Publication Date: 2015-09-02INST OF PLANT PROTECTION FAAS
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2015-09-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a long-time preservation method of a magnaporthe oryzae strain. Rice stalks are taken as the preservation carriers to preserve the magnaporthe oryzae; specifically, a culture medium is poured onto a culture dish flat plate; after the culture medium is solidified, the rice stalks are evenly placed on the culture medium immediately; after the rice stalks absorb the condensate of the culture medium and few nutrient substances carried in the condensate, the magnaporthe oryzae strain is inoculated to the center of the culture medium without rice stalks and enabled to grow and reproduce on the rice stalks; next, the rice stalks with the magnaporthe oryzae are dried at a constant temperature of 30 DEG C and then placed at a temperature within the range of -80 DEG C to 4 DEG C, and therefore, the long-time preservation of the magnaporthe oryzae strain is realized. The preservation method is simple, convenient and quick to operate; the treated magnaporthe oryzae strain is long in preservation period and stable in strain characters; besides, regular transplant is not required in the preservation period, and the preservation cost and the preservation space can be saved; the preservation method is safe and reliable, and also capable of activating the strain more conveniently; in short, the preservation method has important application value for study on the biological characteristics of the magnaporthe oryzae and the development of rice disease-resisting breeding.
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Description

technical field

[0001] The invention belongs to the technical field of microbiology and plant protection, and in particular relates to a method for long-term preservation of rice blast fungus strains. Background technique

[0002] Magnaporthe oryzae Magnaporthe grisea Rice blast caused by Barr. is one of the main diseases of rice, and the annual rice loss caused by it can account for 10%-30% of the total rice output, which is one of the key factors hindering high and stable rice yield. According to the period of onset and the location where rice blast fungus infects rice, rice blast can be divided into seedling blast, leaf blast, panicle blast and grain blast, among which the panicle blast has the most serious impact on rice yield and quality. . Planting disease-resistant varieties is the most economical and effective measure to control rice blast, but a single disease-resistant rice variety will easily lose its disease resistance after 3-5 years of widespread planting, re...

Examples

Embodiment 1

[0032] A long-term preservation method for rice blast fungus strains, specifically comprising the steps of:

[0033] Step 1: Pour the sterilized soluble starch medium into a sterilized 90mm-diameter petri dish on the ultra-clean workbench, and quickly cut the sterilized rice with a length of 20mm after the medium solidifies The rods are evenly placed on the culture medium; the composition of the soluble starch medium is: 10.0 g of soluble starch, 2.0 g of yeast extract, CaCO 3 3.0g, agar powder 16.0g, distilled water 1000.0ml, pH6.5;

[0034] Step 2: Use a sterilized inoculation needle to pick a piece of Magnaporthe grisea bacteria block with a diameter of 5mm to be preserved on the ultra-clean workbench, and place it in the center of the soluble starch medium without the rice stalk, so that the hyphae face down;

[0035] Step 3: Place the petri dish plate in a constant temperature incubator and incubate at a constant temperature of 25°C until the culture dish is covered wi...

Embodiment 2

[0042] A long-term preservation method for rice blast fungus strains, specifically comprising the steps of:

[0043] Step 1: Pour the sterilized soluble starch medium into a sterilized 90mm-diameter petri dish on the ultra-clean workbench, and quickly cut the sterilized rice with a length of 20mm after the medium solidifies The rods are evenly placed on the culture medium; the composition of the soluble starch medium is: 10.0 g of soluble starch, 2.0 g of yeast extract, CaCO 3 3.0g, agar powder 16.0g, distilled water 1000.0ml, pH6.5;

[0044] Step 2: Use a sterilized inoculation needle to pick a piece of Magnaporthe grisea bacteria block with a diameter of 5mm to be preserved on the ultra-clean workbench, and place it in the center of the soluble starch medium without the rice stalk, so that the hyphae face down;

[0045] Step 3: Place the petri dish plate in a constant temperature incubator and incubate at a constant temperature of 25°C until the culture dish is covered wi...

Embodiment 3

[0052] A long-term preservation method for rice blast fungus strains, specifically comprising the steps of:

[0053] Step 1: Pour the sterilized soluble starch medium into a sterilized 90mm-diameter petri dish on the ultra-clean workbench, and quickly cut the sterilized rice with a length of 20mm after the medium solidifies The rods are evenly placed on the culture medium; the composition of the soluble starch medium is: 10.0 g of soluble starch, 2.0 g of yeast extract, CaCO 3 3.0g, agar powder 16.0g, distilled water 1000.0ml, pH6.5;

[0054] Step 2: Use a sterilized inoculation needle to pick a piece of Magnaporthe grisea bacteria block with a diameter of 5mm to be preserved on the ultra-clean workbench, and place it in the center of the soluble starch medium without the rice stalk, so that the hyphae face down;

[0055] Step 3: Place the petri dish plate in a constant temperature incubator and incubate at a constant temperature of 25°C until the culture dish is covered wi...