Collagen/silk fibroin composite support and manufacturing method and application thereof

Abstract

The invention discloses a collagen / silk fibroin composite support and a manufacturing method and application thereof. The collagen / silk fibroin composite support comprises a collagen-based porous support body and fibroin non-woven fabric. The fibroin non-woven fabric is closely integrated in the collagen-based porous support body. The collagen-based porous support body is provided with an aperture structure of 30-500 [u]m. The fibroin non-woven fabric is provided with a good three-dimensional porous structure and has good permeability. The aperture ranges from 20 [u]m to 3,000 [u]m. The diameter of single bright silk is about 10 [u]m, and generally ranges from 8 [u]m to 12 [u]m. The collagen / silk fibroin composite support obtained through the manufacturing method is excellent in biocompatibility and mechanical performance and is provided with a non-woven fabric structure with certain biodegradability. Tissue engineering skin construction can be met well. Then, skin wound healing is accelerated, and healing quality is improved.

Description

technical field [0001] The invention relates to a tissue engineering scaffold, in particular to a collagen / silk fibroin composite scaffold, a preparation method and an application thereof. Background technique [0002] Once the dermis is damaged, it cannot be completely regenerated. In clinical practice, autologous, allogeneic or xenograft skin grafts are usually used to repair large areas of deep skin damage. Or it is difficult to heal by itself; on the other hand, the sources of skin grafts available for transplantation are limited, and there may be adverse reactions such as infection and rejection, and sometimes it is difficult to achieve the ideal repair effect. The basic idea of ​​skin tissue engineering is to rebuild damaged dermal tissue to make dermal regeneration possible. [0003] Silk fibroin is one of the outstanding representatives of natural biological materials. It is safe, non-toxic, non-irritating, and has unique mechanical properties and good biological ac...

AI Technical Summary

Problems solved by technology

[0004] Collagen is the substantive tissue that constitutes the largest amount of connective tissue protein, and is the main component of the extracellular matrix. Its ultrastructure and stability play an extremely important role in cell-cell and cell-matrix interactions; it has a wide range of Its activity and good biocompatibility make it an ideal material for tissue engineering. However, the tissue engineering scaffold constructed solely with collagen has a faster degradation rate and is relatively weak in strength, so it is difficult to meet the requirements of skin tissue engineering scaffolds.

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