Preparation and application of quaternary ammonium tannin magnet modified flocculant for collecting microalgae
A salinized tannin magnetic and salinized tannin technology, which is applied in the field of preparation of quaternary ammonium salinized tannin magnetically modified flocculants, can solve the problem that the magnetic medium cannot be reused, the synthesis of the magnetic separation medium is complicated, and it cannot be re-cultivated, etc. problems, to achieve the effect of low requirements for separation equipment, lower operation and collection costs, and easy control
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Embodiment 1
[0052] Example 1: 100mg / L quaternized tannins and 10mg / mL Fe 3 o 4 Magnetic particle collection of Scenedesmus obliquus ( Scenedesmus obliqnus )
[0053] Adding Fe to the quaternized tannin aqueous solution 3 o 4 Magnetic particles to quaternize tannin powder: Fe 3 o 4 Magnetic particles: water = 1mg: 100mg: 0.4mL, the two are evenly mixed for 1min and then added to a cell density of 1.79×10 10 In the Scenedesmus oblique algae liquid of individual / L, make the final concentration of quaternary ammonium salted tannin be 100mg / L, Fe 3 o 4 The final concentration of magnetic particles was 10 mg / mL. Stir rapidly at 100rpm for 1min and then place it on a 2000G magnetic field to settle. After 1min, calculate the algae cell density in the supernatant and obtain a separation rate of 95.1%.
Embodiment 2
[0054] Example 2: 20mg / L quaternized tannins and 5mg / mL Fe 3 o 4 Magnetic particles collect Chlorella vulgaris ( Chlorella vulgaris)
[0055] Adding Fe to the quaternized tannin aqueous solution 3 o 4 Magnetic particles to quaternize tannin powder: Fe 3 o 4 Magnetic particles: water = 1mg: 250mg: 1mL, the two are evenly mixed for 1min and then added to a cell density of 3.09×10 9 In the Chlorella vulgaris algae liquid of individual / L, make the final concentration of quaternary ammonium salted tannin be 20mg / L, Fe 3 o 4 The final concentration of magnetic particles was 5 mg / mL. Stir rapidly at 100rpm for 1min and then place it on a 3000G magnetic field to settle. After 1min, calculate the algae cell density in the supernatant and obtain a separation rate of 93.6%.
Embodiment 3
[0056] Example 3: 300mg / L quaternized tannins and 10mg / mL Fe 3 o 4 Magnetic particle collection of Staphylococcus branici ( Botryococcus braunii )
[0057] Adding Fe to the quaternized tannin aqueous solution 3 o 4 Magnetic particles to quaternize tannin powder: Fe 3 o 4 Magnetic particles: water = 1mg: 33mg: 0.6mL, the two are evenly mixed for 1min and then added to a cell density of 3.19×10 10 In the staphylococcus brown algae liquid of individual / L, make the final concentration of quaternary ammonium salted tannin be 300mg / L, Fe 3 o 4 The final concentration of magnetic particles was 10 mg / mL. , stirred rapidly at 100rpm for 1min and then placed in a 3000G magnetic field to settle. After 1min, the algae cell density in the supernatant was calculated and the separation rate was 98.2%.
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