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Preparation method of iturinA micro capsule

A technology of iturin and microcapsules, applied in the field of preparation of iturin A microcapsules, can solve problems such as no reports of iturinA microcapsules, and achieve prolonged bacteriostatic efficacy and good storage stability , good dispersion effect

Inactive Publication Date: 2015-10-28
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no report about iturin A microcapsules

Method used

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  • Preparation method of iturinA micro capsule
  • Preparation method of iturinA micro capsule
  • Preparation method of iturinA micro capsule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A preparation method of iturin A microcapsules, comprising the following steps:

[0029] (1) Preparation of microcapsule wall material aqueous solution:

[0030] I. according to mass percentage, prepare 1.25% sodium alginate aqueous solution, for subsequent use;

[0031] II. Prepare a 1.25% aqueous solution of γ-polyglutamic acid according to the mass percentage, and set aside.

[0032] (2) Preparation of microcapsule emulsion:

[0033] Take 200ml of the 1.25% sodium alginate aqueous solution prepared by step (1), slowly add 25ml, 128μg / ml iturin A aqueous solution under 40°C, 495r / min magnetic stirring, and stir for 20min under the same conditions, then slowly add the step (2) Prepare the aqueous solution of 200ml 1.25% gamma-polyglutamic acid, keep stirring for 5h, 20Mpa high-pressure homogenization (GYB30-6D type, Shanghai Donghua high-pressure homogenizer factory) 15min, obtain iturin A microcapsule emulsification liquid.

[0034] (3) The iturin A microcapsule e...

Embodiment 2

[0041] Apparent morphology of iturin A microcapsules

[0042] Sample preparation (ion sputtering instrument) → electron microscope scanner (SEM) observation

[0043] Iturin A microcapsules scanning electron microscope picture see figure 1 . It can be seen from the figure that the iturin A microcapsules have good dispersibility, are spherical, have depressions on the surface, and the particle size is relatively uniform, with a diameter of about 1.0-10.0 μm.

Embodiment 3

[0045] Determination of embedding rate of iturin A microcapsules

[0046]

[0047] I. Extraction of iturin A in microcapsules

[0048] Add 10ml of methanol (chromatographically pure) to 0.20g of microcapsules, infiltrate on a shaker at 37°C and 160r / min for 4h, treat with ultrasonic wave for 40min, centrifuge (8000r / min, 4°C, 10min) and take 1.0ml of the supernatant, and filter it with a filter head Sterilize twice, collect the filtrate, and store in a refrigerator at 4°C.

[0049] II. High performance liquid chromatography (HPLC) measures the embedding rate of iturin A microcapsules

[0050] Mobile phase: 40% acetonitrile aqueous solution, add dipotassium hydrogen phosphate 10mM, adjust pH to 7 with phosphoric acid. The detection wavelength is 200 nm, the flow rate is 0.8 ml / min, and the injection volume is 20 μl.

[0051] The embedding effect of the iturin A microcapsules prepared in Example 1 is shown in Table 1.

[0052]Preparation parameters and embedding rate of t...

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Abstract

The invention discloses a preparation method of iturinA micro capsule, and belongs to the technical field of agricultural microbiology. The iturin A micro capsules employs an aqueous solution of an active substance iturin A as core material, and sodium alginate and gamma-polyglutamic acid as wall materials; the aqueous solution of the core material and the aqueous solution of the wall materials are uniformly mixed by stirring, high pressure homogenization is carried out for 15 minutes, a stable emulsion is obtained, the iturin A micro capsule is prepared by a spray drying technology. The micro capsule prepared by the invention can effectively prevent influences of extraneous ultraviolet ray, temperature and other adverse environments to the biological activity of iturin A, has good slow controlled releasing effect and storage stability, and has good application prospect in the field of biological pesticide.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a preparation method of iturin A microcapsules. Background technique [0002] Microcapsule technology is a technology that uses polymer materials to coat solids, liquids or gases into microcapsules with semi-permeable or sealed capsules. Microcapsules can change the state, quality, volume and performance of materials, protect sensitive components, enhance stability, control the release of core materials, reduce or cover up bad taste, reduce volatility, and isolate components. Microcapsule technology is used in food, It is widely used in medicine, textile, paint, agriculture, cosmetics industry and so on. [0003] Iturin A (iturin A) is an amphiphilic compound with a cyclic lipopeptide structure produced by Bacillus subtilis, consisting of 7 amino acids and 1 β-amino fatty acid. Iturin A contains 3 homologues with molecular weights of 1043Da, 1057...

Claims

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Application Information

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IPC IPC(8): A61K9/50A61K38/12A61P31/10
Inventor 张吉斌曹玉郑龙玉蔡珉敏喻子牛
Owner HUAZHONG AGRI UNIV
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