Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nano-cellulose/cellulose triacetate composite nano-fiber membrane capable of adsorbing and desorbing proteins

A technology of composite nanofibers and triacetate cellulose, which is applied in the field of protein separation and purification, can solve the problems of increasing processing costs, restricting expansion, and increasing technological processes, and achieves the advantages of improving adsorption, optimizing size and distribution, and increasing protein adsorption Effect

Active Publication Date: 2015-11-11
BEIJING INSTITUTE OF TECHNOLOGYGY +1
View PDF8 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This material can be widely used in microfiltration, ultrafiltration, reverse osmosis, etc., but it is rarely reported in the field of protein affinity purification
[0004] Cellulose triacetate is a cellulose derivative obtained by esterification of cellulose under the action of a catalyst; due to the high content of acetyl groups in this material, it has no specific adsorption on proteins, but if it is chemically grafted with affinity groups It will increase the process flow, increase the processing cost, and greatly limit its expansion in the field of separation and purification applications.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nano-cellulose/cellulose triacetate composite nano-fiber membrane capable of adsorbing and desorbing proteins
  • Nano-cellulose/cellulose triacetate composite nano-fiber membrane capable of adsorbing and desorbing proteins
  • Nano-cellulose/cellulose triacetate composite nano-fiber membrane capable of adsorbing and desorbing proteins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Weigh 1.2g of cellulose triacetate, 9mL of dimethyl sulfoxide and 3mL of chloroform solvent (solvent volume ratio 3:1) into a 20mL Erlenmeyer flask, and stir magnetically at room temperature for 8-12h to form a transparent homogeneous spinning Silk liquid: After stirring evenly, let the spinning liquid stand for degassing for 8 hours, then pour it into a 20mL syringe equipped with a #7 needle, fix it on a double-channel micro-injection pump, set the injection speed to 1-4mL / h, and adjust A high-voltage generator keeps the voltage at 20-25kV and the humidity at 45%-55%. The needle droplet quickly forms a Taylor cone, and a triacetate cellulose nanofiber film is formed on the conductive receiving plate with aluminum foil. Then, soak the membrane in deionized water for 24 hours to remove residual solvents and impurities, and dry it in an oven at 60°C to obtain pure cellulose triacetate nanofiber membrane 1, the scanning electron microscope image of which is shown in figure...

Embodiment 2

[0044] (1) Add 10g of wood pulp to the oxidation system of TEMPO (0.05g), NaBr (0.5g) and NaClO (12g), adjust the pH to around 10 with NaOH and react for 6h, then obtain nanocellulose by washing, filtering, and ultrasonic centrifugation Water suspension (0.2% solid content);

[0045] (2) Dissolve 0.74 g of cellulose triacetate in 8.03 g of dimethyl sulfoxide, stir magnetically at 80° C. for 12 hours, then stop stirring, keep warm and stand for defoaming (mass fraction of substrate is 5%);

[0046] (3) Weigh 1.85g of the nanocellulose aqueous suspension obtained in step (1), then slowly drop 1.85g of dimethyl sulfoxide solvent into the suspension, and then vacuumize and rotary evaporate at 80°C for 4h to obtain nanocellulose Dimethyl sulfoxide suspension;

[0047] (4) Slowly add 1.85 g of the nanocellulose dimethyl sulfoxide suspension obtained in step (3) dropwise into the solution in step (2), stir magnetically at 80°C for 4 hours, then drop the temperature to 40°C and add 4...

Embodiment 3

[0053] (1) Add 10g of wood pulp to the oxidation system of TEMPO (0.05g), NaBr (0.5g) and NaClO (12g), adjust the pH to around 10 with NaOH and react for 6h, then obtain nanocellulose by washing, filtering, and ultrasonic centrifugation Water suspension (0.2% solid content);

[0054] (2) Dissolve 0.74 g of cellulose triacetate in 6.17 g of dimethyl sulfoxide, stir magnetically at 80°C for 12 hours, then stop stirring, keep warm and stand for defoaming;

[0055] (3) Weigh 3.71g of the nanocellulose aqueous suspension obtained in step (1), slowly add 3.71g of dimethyl sulfoxide solvent into the suspension, and then vacuumize and rotary evaporate at 80°C for 4h to obtain nanocellulose Dimethyl sulfoxide suspension;

[0056] (4) Slowly add 3.71 g of the nanocellulose dimethyl sulfoxide suspension obtained in step (3) dropwise into the solution in step (2), stir magnetically at 80°C for 4 hours, then drop the temperature to 40°C and add 4.22g of chloroform , to obtain nanocellulo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Adsorption capacityaaaaaaaaaa
Adsorption capacityaaaaaaaaaa
Adsorption capacityaaaaaaaaaa
Login to View More

Abstract

The invention discloses a nano-cellulose / cellulose triacetate composite nano-fiber membrane capable of adsorbing and desorbing proteins and aims at obtaining a novel fiber membrane material applicable to protein separation and purification. Cellulose triacetate is taken as the substrate of the composite membrane; TEMPO method nano-cellulose is introduced into the composite system by virtue of solvent displacement, and the nano-cellulose / cellulose triacetate composite nano-fiber membrane capable of adsorbing and desorbing proteins is prepared by use of a electro-spinning technology. The adsorption capacity of the prepared nano-cellulose / cellulose triacetate composite nano-fiber membrane to the bovine serum albumin can reach 349mg / g, and after the adsorption-desorption process is circulated for three times, above 80% of the desorption rate still can be remained; the composite nano-fiber membrane can be widely applied to protein purification and separation.

Description

technical field [0001] The invention relates to a nanocellulose / triacetate cellulose composite nanofiber membrane capable of adsorbing and desorbing proteins, which belongs to the technical field of protein separation and purification. Background technique [0002] "Electrospinning technology" means that under the action of high-voltage electrostatic force, the polymer solution droplets overcome their own surface tension to form Taylor cones, and then are fully stretched, split into thousands of nanofibers in an instant, and finally form a Microfiber Membrane Method. By optimizing the spinning process conditions, the scale range of the fiber can be effectively regulated, as small as less than 100nm to form a nanostructure, and as thick as greater than 1μm to form a reinforced fiber. The fiber membrane material produced by this technology has a high specific surface area, a wide range of pore diameters, a rich pore structure, a high degree of pore penetration, and is easy to...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): D04H1/728D01D5/00D01D1/02B01D15/08
Inventor 邵自强兰天吕妍妍王飞俊王文俊杨荣杰刘燕华周振文
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com