A lysosome-targeted hypochlorous acid molecular fluorescent probe and its preparation method and application

A fluorescent probe, hypochlorous acid technology, applied in the field of analytical chemistry, can solve the problems of lack of hypochlorous acid detection tools, lack of targeting of hypochlorous acid, inability to detect hypochlorous acid, etc., and achieve strong resistance to other molecular interference. , the effect of high coincidence rate and simple post-processing process
CN105086998BInactive Publication Date: 2017-03-29UNIV OF JINAN
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
UNIV OF JINAN
Publication Date
2017-03-29
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a lysosome targeted fluorescence probe for detecting hypochlorous acid molecules, as well as a preparation method and application thereof, and belongs to the technical field of analytical chemistry. The molecular formula of the fluorescence probe is C37H35N5O5S, with a structure represented by formula I shown in the description. The synthesis of the probe includes four steps, and the after-treatment process is relatively simple; the selective quick detection of the hypochlorous acid molecule probe is achieved, the selectivity is good, and the capability of resisting interference from other molecules is strong; in addition, the change in color of the solution can be observed with the naked eyes, and the change in fluorescence color can also be observed under an ultra-violet lamp, therefore, the fluorescence probe for detecting hypochlorous acid molecules is one with the chromogenic sensing function, can be applied to detection of hypochlorous acid in lysosome in the cells of the biological system, and can be used as a probe for detecting hypochlorous acid in lysosome in the cells. The lysosome targeted fluorescence probe for detecting hypochlorous acid molecules, provided by the invention, is a simple, quick and sensitive reagent for detecting the hypochlorous acid molecules, and has a wide application prospect in the field of biomolecule detection.
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Description

technical field

[0001] The invention relates to a fluorescent probe for rapidly detecting hypochlorous acid in lysosome targets in cells, a preparation method and application thereof, and belongs to the technical field of analytical chemistry. Background technique

[0002] Reactive oxygen species (ROS) are some oxygen-containing free radicals (hydroxyl free radicals and superoxide anion free radicals) and non-free radicals (hypochlorous acid and hypochlorous acid, etc.) that play a very important role in many physiological and pathological processes in organisms. ) collectively. Various ROS are produced by enzymatic and non-enzymatic reactions in organisms under physiological and pathological conditions such as oxidative stress and inflammation. Modern biomedical research shows that the ROS produced in the body is closely related to the occurrence and development of some diseases and the aging of the body. Hypochlorous acid, whose chemical formula is HClO, is an unstable w...

Claims

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