QK-polypeptide-encapsulating polylactide copolymer electrospinning fibrous membrane and preparing method

A technology of polylactide and fiber membranes, applied in medical science, prostheses, etc., can solve the problems of not being able to effectively control the loading and release of QK polypeptides, and achieve good biocompatibility and degradability, and the preparation process The effect of simple operation and simple preparation process

Inactive Publication Date: 2015-12-02
TIANJIN UNIV
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  • Abstract
  • Description
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Problems solved by technology

[0004] The existing QK peptide-loaded materials are generally hydrogels, which cannot effectively control the loading and release of QK peptides
The QK polypeptide is loaded into the electrospun fiber membrane for protection and sustained release, thereby promoting the proliferation of vascular endothelial cells and used for the regeneration of small-caliber artificial blood vessels, which has not been reported in the literature so far

Method used

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  • QK-polypeptide-encapsulating polylactide copolymer electrospinning fibrous membrane and preparing method
  • QK-polypeptide-encapsulating polylactide copolymer electrospinning fibrous membrane and preparing method

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preparation example Construction

[0017] The preparation method of the polylactide copolymer fiber membrane that packs QK polypeptide, the steps are as follows:

[0018] (1) Use phosphate buffered saline (PBS) to prepare an aqueous phase solution (W) at room temperature, which contains QK polypeptide (a VEGF mimic peptide, synthesized by Jill Biochemical (Shanghai) Co., Ltd.) 100-500 μg / mL, thiolated chitosan Sugar 0~6mg / mL, polyethylene glycol diacrylate 0~376μg / mL, bovine serum albumin 1~4mg / mL;

[0019] (2) Polyethylene glycol-b-poly(lactide-co-ε-caprolactone) or poly(glycolide-co-lactide) dissolved in chloroform / N,N-dimethylformamide In the mixed solvent (volume ratio 8:2), prepare a solution with a concentration of 140-180mg / mL as the oil phase solution (O);

[0020] (3) The water phase solution prepared in step (1) and the oil phase solution prepared in step (2) have a volume ratio of 1:15 to 1:25, and the water phase is added to the oil phase, and the volume of the oil phase Add 8-10 mg / mL of F127 as ...

Embodiment 1

[0023] Weigh 6 mg of thiolated chitosan with a weight-average molecular weight of 50,000, 376 μg of polyethylene glycol diacrylate, 500 μg of QK polypeptide (an analog peptide of VEGF, synthesized by Jill Biochemical (Shanghai) Co., Ltd., the same below) and dissolve it in 1 mL of phosphate The buffer was used as the aqueous phase, and 4 mg of bovine serum albumin was added for protection. Weigh 900mg of PELCL and dissolve it in a mixed solvent of 4mL of chloroform and 1mL of N,N-dimethylformamide as an oil phase solution. Dissolve 50 mg of F127 in the above oil phase solution. Take 0.2mL of the above-prepared water phase and add it to 5mL of the above-prepared oil phase, stir (2000r / min) and mix for 20-40 minutes to form a W / O dispersion. The prepared W / O dispersion was electrospun using a single-needle electrospinning device with a voltage of 19kV, a flow rate of the dispersion of 0.4mL / h, and a receiving distance of 18cm. After 12 hours, a fiber film with a fiber diameter ...

Embodiment 2

[0025]Weigh 3 mg of thiolated chitosan with a weight average molecular weight of 100,000, 225 μg of polyethylene glycol diacrylate, and 300 μg of QK polypeptide were dissolved in 1 mL of phosphate buffer as the aqueous phase, and 3 mg of bovine serum albumin was added for protection. Weigh 800mg of PELCL and dissolve it in a mixed solvent of 4mL of chloroform and 1mL of N,N-dimethylformamide as an oil phase solution. Dissolve 45 mg of F127 in the above oil phase solution. Add 0.25mL of the above-prepared water phase to 5mL of the above-prepared oil phase, stir (2000r / min) and mix for 20-40 minutes to form a W / O dispersion. The prepared W / O dispersion was electrospun using a single-needle electrospinning device with a voltage of 16kV, a flow rate of the dispersion of 0.3mL / h, and a receiving distance of 16cm for electrospinning. After 12 hours, a fiber film with a fiber diameter of 400nm to 600nm was collected. , and its thickness is 100 μm to 200 μm. The actual loading capac...

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Abstract

The invention relates to a QK-polypeptide-encapsulating polylactide copolymer electrospinning fibrous membrane and a preparing method. The preparing method of the polylactide copolymer electrospinning fibrous membrane includes the steps that a fibrous membrane body with the thickness of 50 micrometers to 200 micrometers is formed by polyethylene glycol-b-poly(lactide-co-epsilon-caprolactone) fibers with the diameter of 100 nm to 900 nm or poly(glycolide-co-epsilon-lactide) fibers with the diameter of 100 nm to 900 nm and chitosan gel particles encapsulated in the fibers, wherein the diameter of the chitosan gel particles is smaller than 100 nm; QK polypeptide, sulfhydrylation chitosan and polyethylene glycol 200 diacrylate are dissolved into a phosphate buffer solution to serve as a water phase (W); polylactide copolymers are dissolved into mixed solvents of chloroform and N,N-dimethylformamide to serve as an oil phase (O); the water phase is added into the oil phase, and stirring is carried out to form W / O dispersion liquid under the effect of emulsifying agents; electrospinning is carried out on the dispersing liquid to obtain the fibrous membrane. According to the QK-polypeptide-encapsulating polylactide copolymer electrospinning fibrous membrane and the preparing method, the preparing process is simple, the good biocompatibility and the good degradability are achieved, and the fibrous membrane and the preparing method can be used for the tissue engineering field and the in-situ drug sustained release field.

Description

technical field [0001] The invention relates to a polylactide copolymer fiber membrane carrying QK polypeptide and a preparation method thereof, belonging to the fiber membrane technology of tissue engineering and in-situ drug controlled release. Background technique [0002] Polyethylene glycol-b-poly(lactide-co-ε-caprolactone) (PELCL) and poly(glycolide-co-lactide) (PLGA) have good biocompatibility, bioavailability Degradability and excellent mechanical properties, and no immunity. Fibrous membranes prepared by electrospinning can be used as scaffold materials for tissue engineering. As a natural polymer, chitosan has good biocompatibility. After thiol modification, it can undergo Michael addition reaction with polyethylene glycol diacrylate to form a gel, which is used to load bioactive factors (TengD, WuZ, ZhangX, WangY, ZhengC, WangZ, LiC. Synthesis and characterization of insitu cross-linked hydrogel based on self-assembled thiol-modified chitosan with PEG diacrylate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/20A61L27/54A61K38/10A61K47/34A61K47/36
Inventor 袁晓燕杨扬周芳赵蕴慧任丽霞
Owner TIANJIN UNIV
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