Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of feeding type probiotics and its application

A technology of micro-ecological preparations and bacterial preparations, which is applied in the field of feeding-type micro-ecological preparations, can solve the problems of prohibition of antibiotics, long time consumption, and large dependence on ruminant adaptability, so as to relieve poisoning symptoms, restore physiological functions, and promote rapid development. reproductive effect

Active Publication Date: 2019-07-12
内蒙古汉恩生物科技有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One of the main prevention and treatment methods for ruminal acidosis is to adjust the diet and feed concentrated feed reasonably, thereby reducing the incidence of rumen acidosis, but this method takes a long time and is highly dependent on the adaptability of ruminants
Antibiotics have a certain effect on inhibiting microbial lactic acid production, but many antibiotics such as monensin and other ionophore antibiotics also inhibit the degradation of roughage by fiber-degrading bacteria; moreover, due to safety issues, antibiotics are gradually banned

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of feeding type probiotics and its application
  • A kind of feeding type probiotics and its application
  • A kind of feeding type probiotics and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1, the preparation of feeding type probiotics

[0042] The feeding type microecological preparation provided in this example is composed of active ingredients and auxiliary materials; the active ingredients are composed of Lactobacillus casei, Candida tropicalis and Bacillus subtilis; the auxiliary materials are made of sodium alginate, calcium chloride and chitosan become.

[0043] The feeding-type microecological preparation provided in this example is prepared according to the following method: mix the Lactobacillus casei inoculum, Candida tropicalis and Bacillus subtilis inoculum to obtain the feeding-type probiotics; Lactobacillus casei inoculum The active ingredient of Lactobacillus casei ACCC10640; the active ingredient of Candida tropicalis ACCC21161; the active ingredient of Bacillus subtilis ACCC02157 .

[0044] Lactobacillus casei inoculum is prepared by the following method: Lactobacillus casei-sodium alginate liquid is added dropwise to calcium...

Embodiment 2

[0061] Embodiment 2, feeding type probiotics is used for the treatment of white goat ruminal acidosis

[0062] Healthy male Anhui white goats aged about 4 months and weighing about 20kg were selected as experimental animals. They were modeled by over-feeding grains and fed with cornmeal at a rate of 40g / kg. Rumen fluid examination, blood test, and feces were checked before and after modeling. Urinary pH and ciliate counts. Select 20 Anhui white goats with successful modeling and randomly divide them into a control group and a feeding-type microecological preparation group (hereinafter referred to as the experimental group). The control group used rehydration [intravenous infusion of normal saline or 5% (mass percentage) glucose water] to increase blood volume, promote blood circulation, and relieve acidosis symptoms; Feed-type probiotic preparations were gavaged, and the feed-type probiotic preparations of Example 1 were suspended in distilled water to obtain feed-type probio...

Embodiment 3

[0107] The optimization of the preparation conditions of the feeding type probiotics of embodiment 3, embodiment 1

[0108] Investigation of microsphere characteristics: When the microspheres of each strain were prepared, they were placed at room temperature for 1 month after preparation, and the bacterial load was measured 2 months later. Weigh 1g of microspheres, stir in sterilized distilled water, filter with filter paper, take the supernatant to measure the number of viable bacteria, which is the surface bacteria content. Take an equal amount of microspheres, crush them in sterilized distilled water, shake at a constant temperature of 37°C for 24 hours, filter with filter paper, take the supernatant to measure the number of viable bacteria, which is the total bacterial count, and the bacterial load = total bacterial count - surface bacteria count.

[0109] 1. The influence of single factor investigation on the bacterial load of microspheres of each strain

[0110] Lactob...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a feeding type micro-ecological preparation and application thereof. Active ingredients of the feeding type micro-ecological preparation consist of Lactobacillus casei, monilia tropicalis and bacillus subtilis. The feeding type micro-ecological preparation is large in bacterium carrying amount, stable in structure, and capable of promoting intermediate propagation of infusorian after a goat is subjected to rumen acidosis, enabling the content of volatile fatty acids in goat rumen fluid to continuously increase, restoring the digestive ability of the goat to cellulose-type feed, shortening the restoration process, and effectively alleviating the rumen acidosis symptom of the goat. By adopting the feeding type micro-ecological preparation, the rumen internal environment of the goat can be quickly rebuilt, the physiological function of the goat is restored, and important significance for animal husbandry production is achieved.

Description

technical field [0001] The invention relates to a feeding type microecological preparation and its application. Background technique [0002] In recent years, with the development of large-scale breeding industry, the proportion of concentrated feed in ruminant diets has increased significantly. Although concentrated feed can significantly improve animal growth performance, long-term large-scale feeding of concentrated feed will cause rumen acidosis in animals. Rumen acidosis can cause the dehydration reaction of the stomach and intestinal epithelium. In severe cases, it can cause the pH value of the blood to decrease, and the body will suffer from dehydration and other serious consequences. Data show that the elimination rate of cows suffering from subacute acidosis can often be higher than 45% every year, and severe acute acidosis can even cause death, and the mortality rate can be as high as 90%. One of the main prevention and treatment methods for rumen acidosis is to a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A23K50/10A23K10/18A23K20/163A23K20/105A23K20/24C12N11/10C12N11/04C12R1/245C12R1/74C12R1/125
Inventor 顾金刚何燕飞赵秀峰韩春杨李世贵刘翠艳龚明波张瑞颖马晓彤张晓霞王宇洲
Owner 内蒙古汉恩生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com