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Preparation method of intelligent antibacterial polypeptide coating layer

A technology of antibacterial polypeptide and coating, applied in the field of preparation of intelligent antibacterial polypeptide coating, to achieve good biocompatibility and biodegradability, wide application range, and reduce the amount of use

Inactive Publication Date: 2016-02-03
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, few studies have paid attention to the glutathione (GSH) level of most Gram-negative bacteria and some Gram-positive bacterial cells also reaching the millimolar (mM) level

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The polylactic acid nanofiber membrane material is placed in the main chamber of the low-temperature plasma generator, and argon gas is introduced after vacuuming, the pressure is adjusted to 10Pa, the electrode distance is 50mm, the gas flow rate is 0.3L / s, and the voltage and current are applied for plasma Body processing with a processing power of 8 watts and a processing time of 3 minutes. The treated polylactic acid nanofiber membrane material was quickly placed in 100 mL of 0.05% by mass concentration of 4,4'-diphenyldithiol in methanol solution for 2 hours to react. The reacted nanofibrous membrane material is fully soaked with methanol and then vacuum-dried. The polylactic acid nanofibrous membrane material containing sulfhydryl groups on the surface obtained from the treatment reacts with thiol-containing antibacterial tridecapeptide (amino acid sequence is KRWRIRVRVIRKC) under the action of horseradish peroxidase and hydrogen peroxide, and the surface is coval...

Embodiment 2

[0026] The titanium sheet is placed in the main chamber of the low-temperature plasma generator, and argon gas is introduced after vacuuming, the pressure is adjusted to 10Pa, the electrode distance is 50mm, the gas flow rate is 0.3L / s, and the voltage and current are applied for plasma treatment. The power is 20 watts and the processing time is 1 minute. The treated polylactic acid nanofiber membrane material was quickly placed in 10 mL of 0.01% by mass concentration of 4,4'-diphenyldithiol in methanol solution for 4 hours to react. The reacted titanium sheet is fully soaked with methanol, dried in vacuum, and reacted with a sulfhydryl-containing antibacterial polypeptide (the amino acid sequence is IRWRIRVWVRRIC) under the action of horseradish peroxidase and hydrogen peroxide to obtain a titanium sheet bound to the surface of the antibacterial polypeptide. When in contact with bacterial cells, the disulfide bond on the surface of the titanium sheet is broken, and the antimi...

Embodiment 3

[0028] The urinary catheter is placed in the main chamber of the low-temperature plasma generator, and argon gas is introduced after vacuuming, the pressure is adjusted to 10Pa, the electrode distance is 50mm, the gas flow rate is 0.3L / s, and the plasma treatment is carried out by applying voltage and current. The processing power is 20 watts and the processing time is 3 minutes. The treated urinary catheter was placed in 100 mL of 4,4'-diphenyldithiol in methanol solution with a concentration of 0.005% by mass to react for 2 hours. After the reacted urinary catheter is fully soaked with methanol and vacuum-dried, it reacts with the sulfhydryl-containing antibacterial polypeptide (the amino acid sequence is KKWKIVVIKWKKC) under the action of horseradish peroxidase and hydrogen peroxide to obtain the surface-bonded antibacterial polypeptide guide urinary catheter.

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PUM

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Abstract

A preparation method of an intelligent antibacterial polypeptide coating layer is a method universally applicable to different substrate materials. The preparation method includes the following steps: firstly, placing the substrate materials in a low temperature plasma generator main body chamber, vacuumizing, then introducing argon, loading with voltage and current, and carrying out plasma treatment; secondly, rapidly placing the substrate materials after treatment in a methanol solution with a certain amount of dimercaptan; and then, carrying out a reaction of the obtained substrate materials having the surface containing sulfydryl and an antibacterial polypeptide containing sulfydryl under an action of an antioxidant, and forming disulfide bonds. When the antibacterial polypeptide coating layer makes contact with bacterial cells, the disulfide bonds on the surface of the substrate materials are broken and are reduced to release the antibacterial polypeptide containing sulfydryl, so as to play a role of sterilization. The antibacterial activity of the antibacterial polypeptide in a free state is higher than that of a same antibacterial polypeptide covalently immobilized on a material surface, and the usage amount of the antibacterial polypeptide can be greatly reduced.

Description

technical field [0001] The invention relates to a method for preparing an intelligent antibacterial polypeptide coating, relates to a method for preparing materials that can be used in biomedicine and the like, and belongs to the field of biomedical materials. Background technique [0002] Problems such as microbial resistance or enhanced drug resistance have become increasingly prominent, and the development of new antibacterial resources has become the focus of researchers. Antimicrobial peptides have a different mechanism of action from traditional antibiotics and drugs. They have the characteristics of broad antibacterial spectrum, strong action for a short time, and are not easy to produce drug resistance. They have received more and more attention. [0003] Most antibacterial peptides mainly act on the cytoplasmic membrane of bacteria, destroying its integrity and producing perforation, causing the bacterial cell contents to overflow out of the cell and die. The phosp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/10A61P31/04C09D5/14A61K38/08
Inventor 姚琛周宾王怡红温智理
Owner SOUTHEAST UNIV
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