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Method for preparing composite membrane for treating leucoderma from autologous follicle melanocytes

A melanocyte and composite membrane technology, which is used in medical preparations without active ingredients, medical preparations containing active ingredients, and drug combinations, etc., can solve the problem of high treatment costs, unsuitable scars and limited skin lesions in children and children and other problems, to achieve the effect of promoting adhesion and proliferation, good cytocompatibility, and improving effect

Active Publication Date: 2016-02-17
GUANGZHOU PUDAO LIANXIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Surgical treatment is the most promising means of curing vitiligo. The traditional surgical treatment is mainly blister epidermis transplantation, perforation transplantation, etc., but there are certain defects: large damage, long treatment time, limited treatment of skin lesions, not suitable for infants and children treatment and scar formation
[0004] At present, the more effective therapy is autologous melanocyte transplantation. Since the obtained melanocytes are derived from the autologous body, it will not cause rejection; it can also be used to treat patients with a large range of skin lesions, which is expected to completely change the treatment difficulties of vitiligo. However, due to autologous Melanocytes are taken from the skin, but they are limited in number, difficult to separate, complicated to cultivate, and difficult to promote.
Although there are indeed some methods for quickly separating cells on the market, because the source of the cells requires a large amount of skin, the transplanted cells are not easy to fix, the distribution is uneven, the curative effect is not exact, and the treatment cost is high, so this method still exists. certain disadvantages

Method used

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  • Method for preparing composite membrane for treating leucoderma from autologous follicle melanocytes
  • Method for preparing composite membrane for treating leucoderma from autologous follicle melanocytes
  • Method for preparing composite membrane for treating leucoderma from autologous follicle melanocytes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Preparation of collagen film: Prepare a collagen solution with a concentration of 5 wt% with an acetic acid solution with a concentration of 1 wt%, stir well, spread it flat and dry to form a film to obtain a pure collagen film ( Figure 4 Middle A).

[0029] (2) Preparation of autologous hair follicle melanocytes: Use a 2mm skin puncher to take 2 scalps containing hair follicles, soak them in 70% alcohol, rinse with PBS solution, rinse with sterile saline until the surface is clean, and immediately add penicillin (400U / mL), streptomycin (400mg / mL) and Tylosin (250mg / mL) in PBS solution for 3 times, then immersed in sterile 0.25% neutral protease, digested at 4°C for 5h and put into new PBS solution , take out the hair follicles under a microscope and place them in the medium, slowly add 0.25% trypsin dropwise, incubate at 37°C for 10 minutes, remove excess trypsin, replace with new medium, 37°C, 5% CO 2 Cell separation can be observed by culturing in a saturated ...

Embodiment 2

[0032] (1) Preparation of collagen film: Prepare a collagen solution with a concentration of 5 wt% with acetic acid solution with a concentration of 1 wt%, stir well, lay flat and dry to form a film to obtain a pure collagen film.

[0033] (2) Preparation of autologous hair follicle melanocytes: same as in Example 1. For the repair area greater than 20cm 2 In the affected area, the cells expanded in vitro were completed for clinical use within 2 weeks.

[0034] (3) Transfer the cultured autologous melanocytes to the surface of the collagen membrane, and then give it to patients with vitiligo. When using it, you only need to simply grind the treatment area, and then paste the autologous melanocytes on the treatment area to complete the treatment; after 4 weeks, you can see obvious recoloration.

Embodiment 3

[0036] (1) Preparation of collagen / PVA composite film: prepare a collagen solution with a concentration of 0.5wt% with an acetic acid solution with a concentration of 0.3wt%, and set aside; weigh PVA, add it to deionized water, and prepare a PVA solution with a concentration of 5wt% , stirred in a constant temperature oil bath at 65°C for 6 hours, added collagen solution after dissolving, prepared a mixed solution with a volume ratio of PVA and collagen of 1:10, stirred well, laid flat and dried to form a film to obtain a collagen / PVA composite film ( Figure 4 Middle B).

[0037] (2) Preparation of autologous hair follicle melanocytes: same as in Example 1. For the repair area less than 20cm 2 In the affected area, the cells expanded in vitro are ready for clinical use within 1 week.

[0038] (3) Transfer the cultured autologous melanocytes to the surface of the collagen / PVA composite membrane, and then give it to patients with vitiligo. When using it, you only need to sim...

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Abstract

The invention discloses a method for preparing a composite membrane for treating leucoderma from autologous follicle melanocytes. Enough autologous melanocytes are separated from follicles of a patient; cells are transferred to a biological membrane to continually grow by using the characteristics that performances and functions of the autologous melanocytes changed after the autologous melanocytes are cultured and amplified, so that the controllability of the cells is effectively improved and clinicians can extremely conveniently use the composite membrane. The defects of a traditional leucoderma treatment surgery that the damages are great, the treatment time is long, the treated skin lesion face is not large, the surgery is not suitable for treating infants and children, and scars are formed and the like are avoided. The method is convenient to operate, has good repeatability and is suitable for large-scale popularization of clinic treatment of the leucoderma.

Description

technical field [0001] The invention relates to a method for preparing a composite film for treating vitiligo by using autologous hair follicle melanocytes. Background technique [0002] Vitiligo is a common skin disease that seriously affects the beauty of patients. About 1% to 2% of the world's population is suffering from Vitiligo. In China, it is conservatively estimated that there are at least 20 million Vitiligo patients. About 30% of patients with this disease do not respond to drugs Or ultraviolet radiation is not sensitive, such patients want to give fast and effective treatment, therefore, at least about 6 million patients will face surgery-related cell therapy. [0003] At present, the means for the treatment of vitiligo mainly include Chinese and Western medicine treatment, various light irradiation treatments and surgical treatment. These treatment methods have their own advantages and disadvantages and indications. Chinese and Western medicine treatment is div...

Claims

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Application Information

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IPC IPC(8): A61K35/36A61K9/70A61P17/00A61K47/42A61K47/32
Inventor 任力李卫昌王迎军
Owner GUANGZHOU PUDAO LIANXIN BIOTECH CO LTD
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