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A method of increasing a fermentation yield of natural abscisic acid

A technology of natural abscisic acid and abscisic acid, applied in the field of microbial fermentation, can solve the problems of easy viscosity of fermentation liquid, high cost of fermentation promoter, insufficient utilization of bacterial cells, etc., and achieve stable fermentation environment, low cost and reduced viscosity. Effect

Inactive Publication Date: 2016-03-16
山东营养源食品科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is that the utilization of oxygen by the bacterium is insufficient, the cost of the added fermentation accelerator is relatively high, and the fermentation liquid is easy to be viscous

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] First, the preserved strains were activated and cultured on a PDA plate, and the prepared PDA medium was sterilized in an autoclave at 121° C. for 30 minutes, and then poured onto the plate for later use. Then inoculate the strains on a solid plate in an ultra-clean workbench, and activate and cultivate at 26-28°C for 4-5 days. Pick the activated bacteria into the liquid seed medium with a sterile inoculation loop, break up and shake well, and shake the flask at 28°C for about 72 hours.

[0022] Insert the strain cultured in the seed culture medium into the shake flask that PD liquid culture medium is housed with 5% inoculation amount and carry out fermentation culture, and the composition and content of PD liquid culture medium are: glucose 20g / L, potato 200g, wash After peeling, cut into cubes, put them in a pot and boil on the induction cooker for 30 minutes, then filter the boiled potatoes with four layers of gauze, and dilute the filtrate to 1L with distilled water...

Embodiment 2

[0025] Strain activation and seed culture method are the same as in Example 1. Put the activated strain into the shake flask equipped with special liquid fermentation medium with 10% inoculation amount for fermentation and cultivation. The composition and content of special liquid fermentation medium are: glucose 20g / L, MgSO 4 ·7H 2 O0.2g / L, KCl0.5g / L, CaCO 3 5g / L, KH 2 PO 4 0.8g / L, vitamin B11mg / L, glutamic acid monosodium salt 3.0g / L, FeSO 4·7H 2 O0.5mg / L, ZnSO 4 ·7H 2 O2.5mg / L, CuSO 4 ·7H 2 O4mg / L, corn flour 4g / L, make up 1L with distilled water. Fully pulverize the pepper leaves, extract according to the weight ratio of 0.1M phosphate buffer solution to the crushed pepper leaf tissue 1:0.1, the extraction temperature is 4°C, and the extraction time is 12 hours. 0.45μm membrane filter for use after filtration; the prepared special liquid fermentation medium was sterilized and divided into two groups, one group was added with capsicum leaf extract at a dosage of 5...

Embodiment 3

[0028] Strain activation and seed culture method are the same as in Example 1. Put the activated strain into the shake flask equipped with special liquid fermentation medium with 10% inoculation amount for fermentation and cultivation. The composition and content of special liquid fermentation medium are: glucose 20g / L, MgSO 4 ·7H 2 O0.2g / L, KCl0.5g / L, CaCO 3 5g / L, KH 2 PO 4 0.8g / L, vitamin B11mg / L, glutamic acid monosodium salt 3.0g / L, FeSO 4 ·7H 2 O0.5mg / L, ZnSO 4 ·7H 2 O2.5mg / L, CuSO 4 ·7H 2 O4mg / L, corn flour 4g / L, make up 1L with distilled water. Divide the prepared special liquid fermentation medium into two groups, add sodium succinate and ammonium molybdate to one group, so that the concentrations of sodium succinate and ammonium molybdate in the special fermentation medium are 5mM / L and 1mM respectively / L, and the other group was not added as a control, and the bacterial species was inserted after sterilization; at 28°C, 150 rpm shaking fermentation culture...

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Abstract

The invention relates to method of increasing a fermentation yield of natural abscisic acid and belongs to the technical field of microorganism fermentation. The method includes inoculating botrytis cinerea capable of producing abscisic acid to a potato dextrose agar (PDA) solid plate, activating, culturing, subjecting the botrytis cinerea activated on the plate to seed culture, inoculating a cultured seed solution to a liquid fermentation culture medium, performing fermentation culture, and adding one or more than two of a plant leach liquor, sodium succinate and ammonium molybdate into the liquid fermentation culture medium, thus increasing the yield of the natural active abscisic acid produced by fungus fermentation. The method increases the oxygen utilization rate of a strain, accelerates thallus growth, and allows the strain to produce the abscisic acid in a high product synthesis rate. Materials used by the method are wide in source. The production cost is reduced. Processes are simplified. Subsequent industrial application is facilitated.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation and relates to a new method for improving the fermentation yield of natural abscisic acid. Background technique [0002] Abscisic acid is one of the five natural growth regulators of plants. It plays many roles in the growth and development of plants, has broad application prospects in agriculture, can improve the drought resistance and salt tolerance of plants, and has extremely high value for the development and utilization of low-yield fields, afforestation, and desert greening; Abscisic acid is also an effective inhibitor of seed germination, so it can be used for seed storage to ensure the storage quality of seeds and fruits; in addition, abscisic acid can also cause the rapid closure of leaf stomata, which can be used to preserve flowers, regulate flowering period, and promote rooting etc. It has great application value in flower gardening. However, due to the extremely low ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/42C12R1/645
Inventor 苏振峰王庆国李艳华贾梦奇王龙龙张兴荣
Owner 山东营养源食品科技有限公司
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