Breast implant three-dimensional structure and rapid forming method thereof

A three-dimensional structure and implant technology, applied in prosthetics, medical science, etc., can solve the problems of unsustainable effect, fat cell necrosis, etc., and achieve the effect of enhancing viability and reducing damage

Inactive Publication Date: 2016-03-30
TIANJIN MIFANG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Autologous transplanted fat particles cannot establish a blood supply system well. When a large amount of fat accumulates together, fat cells will be necrotic, dissolved, and absorbed due to insufficient blood supply, which can easily cause infection, hematoma, deformation, pain, induration, cysts, Necrosis and other sequelae [Vallée M, Cote

Method used

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  • Breast implant three-dimensional structure and rapid forming method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1) Material preparation

[0039] Take 0.1g of sodium alginate, 1g of fibrinogen and 20g of gelatin and place them in 100mL of culture medium to dissolve them completely, and sterilize them to obtain gelatin, sodium alginate and fibrinogen solutions; prepare 0.01g / ml of calcium chloride Aqueous solution is used as sodium alginate cross-linking agent, 0.01g / ml thrombin is used as fibrinogen cross-linking agent; endothelial cells (ECs), nerve cells and adipose stem cells (ADSCs) are extracted, cultured and passaged; endothelial cells (ECs), nerve cells Cells and adipose stem cells (ADSCs) were mixed with gelatin, sodium alginate, and fibrinogen solutions, and the density of endothelial cells was 1×10 4 cells / mL, the density of nerve cells was 1×10 5 cells / mL, the density of adipose-derived stem cells was 2×10 6 individual / mL; add 1% endothelial cell growth factor solution in the sodium alginate solution containing adipose-derived stem cells; dissolve the degradable polyc...

Embodiment 2

[0047] 1) Material preparation

[0048] Take 0.1g of sodium alginate, 20g of fibrinogen and 4g of gelatin and place them in 100mL culture medium to dissolve them completely, and then sterilize them to obtain gelatin, sodium alginate and fibrinogen solutions; prepare 1g / ml calcium chloride aqueous solution As a sodium alginate cross-linking agent, 1g / ml thrombin as a fibrinogen cross-linking agent; extract patient bone marrow stem cells (BDSCs), culture and passage; part of the bone marrow stem cells (BDSCs) induced transformation into endothelial cells and nerve cells; The remaining part of bone marrow stem cells (BDSCs), endothelial cells (ECs) and nerve cells were mixed with gelatin, sodium alginate and fibrinogen solution respectively, and the density of bone marrow stem cells (BDSCs) was 1×10 3 cells / mL, the density of endothelial cells (ECs) was 1×10 4 cells / mL, the density of nerve cells was 1×10 5 pcs / mL; dissolving degradable polyurethane in 1,4-dioxane to obtain a s...

Embodiment 3

[0056] 1) Material preparation

[0057] Take 2g of sodium alginate, 1g of collagen and 10g of gelatin and place them in 100mL of culture medium to dissolve them completely, and sterilize them to obtain sodium alginate, collagen and gelatin solutions; prepare 10g / ml of calcium chloride aqueous solution as sodium alginate gelatin Induced pluripotent stem cells were extracted from patients, cultured and passaged; the induced pluripotent stem cells were mixed with sodium alginate, collagen, and gelatin solutions and divided into three parts; the induced pluripotent stem cells and natural polymer solutions were divided into three parts Add 1%, 5%, 10% by volume of endothelial cell growth factor, nerve cell growth factor, adipocyte growth factor; dissolve degradable lactic acid and glycolic acid copolymer (PLGA) in tetraethylene glycol to obtain mass volume concentration It is a 20% synthetic polymer solution, which is sterilized and ready for use; cell culture fluid is prepared.

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Abstract

The invention provides a breast implant three-dimensional structure and a rapid forming method thereof and belongs to the field of manufacturing of medical equipment, biological materials and organs. A breast implant comprises nerve fiber bundles, blood vessels and a mammary gland system, wherein the nerve fiber bundles and the blood vessels are formed by commixing and printing a synthesized macromolecular solution, a natural macromolecular solution, growth factors and human cells, and then culturing; the mammary gland system comprises adipose tissue, a mammary gland duct and a nipple, and are formed by commixing and printing a synthesized macromolecular solution, growth factors and human stem cells, and then transforming; and the mammary gland system is distributed between a breast dermic layer and chest tissues. By utilizing a composite multi-nozzle 3D printing technology, integrated three-dimensional construction of different materials in different directions is realized; a novel concept and a novel method are provided for rapidly manufacturing an artificial breast implant in vitro; and the disadvantages that structures of previous other forming methods are too simple and the process operation is complicated are overcome, and physiological functions of a plurality of types of structure tissues in a human breast can be simulated very well.

Description

technical field [0001] The invention belongs to the technical field of artificial manufacturing of complex organs of organisms, and involves the use of cells, synthetic polymer materials, cell matrix materials and 3D printing technology and process methods, which is different from previous tissue engineering processes. Background technique [0002] Female breasts have dual functions of lactation, feeding and aesthetics. Due to congenital dysplasia, tumor resection, trauma and other reasons, female micromastia and breast loss will seriously affect the patient's body and mind. According to the American Society of Orthopedic Surgeons, more than 5 million people worldwide underwent breast reconstruction surgery in 2004, 4 million of which were due to tumor resection [2004 American Society of Plastic Surgeons Statistics. Available from: www.plasticsurgery.org]. Breast cancer is one of the most common malignant tumors in women, with an incidence rate of 10-15%, and it is increasin...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/38A61L27/54A61L27/18A61L27/16A61L27/20A61L27/22A61L27/24
CPCA61L27/16A61L27/18A61L27/20A61L27/22A61L27/222A61L27/24A61L27/3666A61L27/367A61L27/3808A61L27/3834A61L27/54C08L89/00C08L5/08C08L5/10C08L23/06C08L23/12C08L27/06C08L27/18C08L67/04C08L69/00C08L71/02C08L75/04
Inventor 刘畅
Owner TIANJIN MIFANG TECH CO LTD
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