A kind of lung substitute and its three-dimensional printing and injection molding manufacturing method

A substitute, 3D printing technology, applied in the direction of biochemical equipment and methods, microorganisms, animal cells, etc., can solve the problems of long working hours, low forming efficiency, and inability to attach to the side surface of the formed body, so as to enhance viability and enhance Effects on suture resistance strength, improved composite efficiency, and physiological function of formed structures

Inactive Publication Date: 2018-10-26
TIANJIN MIFANG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] This kind of forming network has the following disadvantages: 1. The forming table can only rely on the three-dimensional motion device to move, and the position parameters are controlled by the mutually perpendicular X-axis and Y-axis when processing materials such as circular cross-section and ring cross-section, and the accuracy needs to be improved.
2. The fixed nozzles are all screw extrusion type and cannot be replaced. There are structural disadvantages in cell assembly or printing processing, and it is impossible to accurately control the number of cells printed, the thickness of the cell layer and the exact position.
3. The forming efficiency is low. The diameter of the slurry sprayed by the motor-assisted rapid prototyping nozzle depends on the diameter of the nozzle. The diameter of the nozzle is generally micron. Huge volume, long working hours
4. It is not possible to spray dilute polymer solutions, single-layer cells, and cell microspheres. The diameter of the slurry sprayed by the motor-assisted rapid prototyping nozzle is generally above 100 microns, but the diameter of higher animal cells is mostly less than 25 microns. ejects multiple layers of cells
5. It is impossible to spray the side surface of the forming body. The motor-assisted rapid prototyping nozzle needs to be installed vertically. If it is installed horizontally, the slurry will drip under its own gravity after being extruded from the nozzle, and cannot be sprayed on the forming body. side surface attachment

Method used

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  • A kind of lung substitute and its three-dimensional printing and injection molding manufacturing method

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Experimental program
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Effect test

Embodiment 1

[0035] 1) 3D printing material preparation

[0036] Take 0.1g chitosan and 1g gelatin and place them in 100mL culture medium to dissolve them completely, and sterilize them to obtain chitosan-gelatin solution; prepare 0.05g / ml sodium superphosphate aqueous solution as chitosan cross-linking agent and cell Culture medium (DMEM medium); extract adipose-derived stem cells (ADSCs) and lung epithelial cells, culture and passage; mix adipose-derived stem cells (ADSCs) with chitosan-gelatin solution, wherein the concentration of adipose-derived stem cells is 1×10 5 each / mL; add 0.1% mass percentage of endothelial growth factor and epithelial growth factor to the fat stem cell-chitosan-gelatin solution; the mass volume concentration obtained by dissolving degradable polyurethane in tetraethylene glycol is 2% Synthetic polymer solution, sterilized for use.

[0037] 2) Preparation of lung epithelial cell microspheres

[0038] Mix the lung epithelial cells and the sodium alginate aqueo...

Embodiment 2

[0046] 1) 3D printing material preparation

[0047] Take 1g of sodium alginate and 20g of gelatin in 100mL of culture medium to dissolve them completely, and sterilize to obtain a sodium alginate-gelatin solution; prepare 1g / ml of calcium chloride aqueous solution as a sodium alginate cross-linking agent and cell culture medium (DMEM culture medium); bone marrow stem cells and lung epithelial cells were extracted, cultured and passaged; bone marrow stem cells were mixed with sodium alginate‐gelatin solution, and the concentration of bone marrow stem cells was 3×10 4 each / mL; bone marrow stem cells-sodium alginate-gelatin solution was added with 0.1% mass percentage of endothelial cell growth factor and cell transfer factor, platelet factor mixture and epithelial cell growth factor; the degradable polycaprolactone was dissolved in four A synthetic polymer solution with a mass volume concentration of 2% was obtained in ethylene glycol, and was sterilized for use.

[0048] 2) Pr...

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Abstract

The invention belongs to the technical field of biomedical materials and organ manufacturing and discloses a lung substitute and a three-dimensional printing and injection molding manufacture method thereof.The lung substitute comprises left and right lung lobes, a tracheal network, a vascular network, alveolus tissues and a lung adventitia.The left and right lung lobes, the tracheal network, the vascular network and the lung adventitia are formed by 3D (three-dimensional) printing, and the alveolus tissues are formed by injection of pulmonary epithelial cell and extracellular matrix solution / microspheres or stem cell, epithelial cell growth factor and extracellular matrix solution among the lung adventitia, the tracheal network and the vascular network by the aid of syringes in a 3D printing process of the lung adventitia, the tracheal network and the vascular network.The lung substitute with the left and right lung lobes, the vascular network and the tracheal network can be connected with human blood vessels and tracheas to realize regenerative repair of lung organs.In addition, by means of multi-nozzle 3D printing and injection forming techniques, rapid formation of heterogeneous materials from different angles and different directions is guaranteed, and compounding efficiency of the heterogeneous materials and physiological functions of formed structures are improved.

Description

technical field [0001] The invention belongs to the technical field of artificial manufacturing of biomedical materials and solid organs, and in particular relates to a method for preparing a transplantable lung containing a bronchial network. Background technique [0002] Organ failure is one of the greatest threats to human beings, and the number of organ donors each year is only one hundred thousandth of the number of patients who need organ transplantation. It has been a dream of human beings for thousands of years to assemble a variety of cells and biomaterials into transplantable living organs through advanced shaping technology. Rapid prototyping, also known as three-dimensional printing (three-dimensional, 3D, printing) or additive manufacturing (additive manufacturing, AM) is an advanced manufacturing technology developed in the 1980s, which can make a variety of cell material units in digital Model-driven automatic assembly into corresponding three-dimensional str...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/38A61L27/18A61L27/50A61F2/02C12N5/0775C12N5/0735
CPCA61F2/02A61F2240/001A61L27/18A61L27/3813A61L27/3839A61L27/3895A61L27/50C12N5/0606C12N5/0625C12N5/0663C12N5/0667C12N2513/00C08L75/04
Inventor 刘畅
Owner TIANJIN MIFANG TECH CO LTD
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