Bacterial fiber material made from bacterial cells, and applications thereof

A bacterial fiber and cell technology, applied in bacteria, fiber chemical characteristics, textiles and papermaking, etc., can solve the problems of short production cycle and low cost

Active Publication Date: 2016-04-06
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of this, the purpose of the present invention is to provide a kind of fiber with short product...

Method used

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  • Bacterial fiber material made from bacterial cells, and applications thereof
  • Bacterial fiber material made from bacterial cells, and applications thereof
  • Bacterial fiber material made from bacterial cells, and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0093] 1. A fibrous material formed by bacterial growth.

[0094] 2. The bacterial fiber according to item 1, which has a length of not less than 50 µm.

[0095] 3. A recombinant bacterial cell used to produce the bacterial fiber described in item 1 or 2.

[0096] 4. The recombinant bacterial cell according to item 3, wherein said recombinant bacterial cell is genetically modified such that it can grow to an increased length compared to the starting bacterium, said genetic modification occurring on the bacterial chromosome and / or on the plasmid.

[0097] 5. The recombinant bacterial cell according to item 4, wherein cell division of the recombinant bacterial cell is inhibited.

[0098] 6. The recombinant bacterial cell according to item 5, wherein expression of a cell division gene in the recombinant bacterial cell is suppressed.

[0099] 7. The recombinant bacterial cell according to item 6, wherein the expression level of one or more cell division genes in the recombinant ...

Embodiment 1

[0333] Example 1 Obtaining bacterial cells and bacterial fibers with increased length by inhibiting the expression of the cell division gene ftsZ

[0334] 1. Obtain recombinant Escherichia coli in which the ftsZ gene on the genome is knocked out and complemented on the plasmid.

[0335] Using the PKD3 plasmid, an auxiliary PKD3 plasmid that complements ftsZ can be obtained by the method described in the following literature (KDaiandJLutkenhaus, ftsZisanessentialcelldivisiongeneinEscherichiacoli.J.Bacteriol.1991,173(11):3500.), thereby obtaining the Escherichia coli genome ftsZ knockout system : E. coli JKD7-1 (PKD3) (publicly available from Tsinghua University). The auxiliary PKD3 plasmid that complements ftsZ is a temperature-sensitive replicon plasmid, which cannot replicate at 42°C. Therefore, this system can be applied to culture at 42°C to obtain bacteria with limited expression of cell division genes.

[0336] 2. Obtain bacterial cells and bacterial fibers with increas...

Embodiment 2

[0338] Example 2 Obtaining length-increased recombinant bacterial cells and bacterial fibers through overexpression of the sulA gene in Escherichia coli EscherichiacoliTrans1T1

[0339] figure 2 Schematic diagram for the generation of E. coli bacterial fibers, the specific steps are as follows:

[0340] 1. Obtain recombinant Escherichia coli cells

[0341] 1. Construction of the vector containing the sulA gene

[0342] The genome of Escherichia coli Trans1T1 (Escherichia coliTrans1T1) (purchased from Beijing Quanshijin Biotechnology Co., Ltd.) was extracted as a template, and the target gene sulA was amplified by PCR using pfu enzyme with sulAF and sulAR as primers. The obtained PCR product was confirmed to be the target gene sulA by sequencing. The sulA fragment of the target gene obtained above was inserted into the vector pet28a (purchased from addgene) by enzyme digestion to obtain the plasmid pet28asulA ( image 3 ).

[0343] Using Pet28aarasulAF and Pet28aarasulAR ...

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Abstract

The present invention relates to the field of materials, particularly to bacterial fibers obtained by changing bacterial form, further to recombinant bacterial cells producing bacterial fibers through genetic molecule operation. The present invention further relates to a method for spinning by using the bacterial fibers, and the obtained textile. According to the present invention, compared with the existing natural fibers, the bacterial fibers of the present invention have advantages of short production cycle, low labor cost and low production cost; compared with the artificial fibers, the bacterial fibers of the present invention have advantages of environmental protection, degradability and the like of the natural fibers; and the bacterial fibers adopted as the textile material have advantages of gloss, softness, ultra-high density, light weight, good water resistance and good moisture permeation, good perspiration wicking action, good warm keeping property, good wear resistance, high cleaning ability, high stain removing ability and the like, and are ultra-fine fibers.

Description

technical field [0001] The invention relates to the field of textiles, in particular to a bacterial fiber material prepared from bacterial cells and its application. Background technique [0002] Fibers are natural or synthetic filamentous substances. In addition to being used as textile materials in the textile industry, fibers can also play a role in medicine, construction, industry and other fields, such as preparing medical textiles with chitin fibers, and combining fiber technology and concrete technology to prepare steel fibers to improve the quality of civil engineering. [0003] Depending on the source, fibers are generally divided into natural fibers and man-made fibers. Natural fibers exist in nature and can be obtained directly. According to their sources, they are divided into three categories: plant fibers, animal fibers and mineral fibers. Plant fibers are fibers obtained from seeds, fruits, stems, leaves, etc. of plants, including cotton, kapok, flax, ramie,...

Claims

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Application Information

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IPC IPC(8): D01F9/00D06N7/04C12N1/21C12P19/04
Inventor 陈国强王颖赵亮谭丹吴弘张星辰蒋笑然
Owner TSINGHUA UNIV
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